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The map label for this gene is gltX

Identifier: 157825574

GI number: 157825574

Start: 441926

End: 443269

Strand: Reverse

Name: gltX

Synonym: A1C_02435

Alternate gene names: 157825574

Gene position: 443269-441926 (Counterclockwise)

Preceding gene: 157825575

Following gene: 157825566

Centisome position: 36.01

GC content: 33.85

Gene sequence:

>1344_bases
ATGACAAAAGTTATAACACGATTTGCTCCGTCACCGACCGGTATGTTACATGTCGGAAATATCAGGGTAGCATTGCTTAA
CTGGCTATATGCAAAGAAACATAACGGGCAGTTTATTTTAAGATTTGACGATACGGATTTAGAGCGTAGTAAACAGGAAT
ATAAAGACGCTATAAAAGACGATTTAAAATTTTTAAATCTTCATTGGGATCAGACATTTAATCAATTAAGTCGTTTAAGT
AGATATGATGAAATCAAAAATCTATTATTAGATAAAAAAAGATTATATGCATGCTATGAGACTCCAGACGAACTAGAATT
AAAGCGTAAATTTCAGTTATCTAAAGGTCTACCGCCGATGTATGACCGTGCTAGCTTAAATCTAACAGAAGAACAAACAG
AAAAATATATAGAACAAGGAAGAAACCCGCATTATAGATTTCTGGTAAATCATGAACCGATTAGCTGGCATGATATGATC
AAAGGCGAAATTCAATATGACGGCAAGGCTTTAAGCGATCCAATAGTGATAAGAGCGGACGGTAGTATGACATATATGTT
ATGCTCGGTTATTGACGATATTGATTATGATATTACTCATATTATTAGGGGTGAAGATCACGTTAGCAATACAGCTATTC
AAATTCAAATGTTTGAGGCTTTAAATAATACTCCTACGACTTTTGGACATTTAAGTTTAATAATCAATAAAGATGAGAAA
ATTTCTAAACGAGTGGGAGGGTTTGAGATCACAACTCTTAGAAAAGAAATCGGGATCGAAGCTATGGCAATAGCTAGCTT
TTTTAGCTTACTTGGTTCATCGGCACAAATCTTGCCTTATAAATCAATGGAGAAGCTAATAAATCAATTTGAGATAAGCA
GTTTTTCTAAAAGCCCGACTATTTATCAACCAAAAGATTTAGAAAGATTAAACCATAAATTATTAATAAGTTTAGATTTT
GACGAAGTAAAAGACCACCTTAAAGAAATAGATGCCGAGTATATTGATGAAAATTTTTGGTTATCGATAAGACCTAACTT
GCAAAAATTACGTGATGTAAAAGACTGGTGGGAAATTTGTCATCACACTCCGAATGTAGAAAGTCTAAATTTAGATCAGG
AATATTTAAAACAAGCAGCAGAATTATTACCGCAAGGTGCAATTACTAAAGATAGCTGGAGCATTTGGACTAAAGAAATA
ACAAATATAACAGGCAGAAAAGGTAAGGAGCTATTTCTACCTCTTCGCCTTGCTTTAACCGGCAGAGAATCAGGACCGGA
AATTGCAGATGTTTTACCTTTGATTAGTAGGGAAGAGATAGTAAAACGATTAACTAGTGCGTAA

Upstream 100 bases:

>100_bases
CCGCTCGCAATGACAATTTTCGAGCGATGCAACAATGCCGGCTTGACCACAGCATGACATAGTTCTTTAATAGGATGACA
ATTAATAAAATTACTTATTT

Downstream 100 bases:

>100_bases
CTCTCTATGTCATTCCTAACTAAAAGCATTATTGCGTGTCTTGAAAAACCTGTTCTATGTCATCCACACGAAAGCTGAGA
TCAACTACTTTAAAACTTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTKVITRFAPSPTGMLHVGNIRVALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIKDDLKFLNLHWDQTFNQLSRLS
RYDEIKNLLLDKKRLYACYETPDELELKRKFQLSKGLPPMYDRASLNLTEEQTEKYIEQGRNPHYRFLVNHEPISWHDMI
KGEIQYDGKALSDPIVIRADGSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQIQMFEALNNTPTTFGHLSLIINKDEK
ISKRVGGFEITTLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLINQFEISSFSKSPTIYQPKDLERLNHKLLISLDF
DEVKDHLKEIDAEYIDENFWLSIRPNLQKLRDVKDWWEICHHTPNVESLNLDQEYLKQAAELLPQGAITKDSWSIWTKEI
TNITGRKGKELFLPLRLALTGRESGPEIADVLPLISREEIVKRLTSA

Sequences:

>Translated_447_residues
MTKVITRFAPSPTGMLHVGNIRVALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIKDDLKFLNLHWDQTFNQLSRLS
RYDEIKNLLLDKKRLYACYETPDELELKRKFQLSKGLPPMYDRASLNLTEEQTEKYIEQGRNPHYRFLVNHEPISWHDMI
KGEIQYDGKALSDPIVIRADGSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQIQMFEALNNTPTTFGHLSLIINKDEK
ISKRVGGFEITTLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLINQFEISSFSKSPTIYQPKDLERLNHKLLISLDF
DEVKDHLKEIDAEYIDENFWLSIRPNLQKLRDVKDWWEICHHTPNVESLNLDQEYLKQAAELLPQGAITKDSWSIWTKEI
TNITGRKGKELFLPLRLALTGRESGPEIADVLPLISREEIVKRLTSA
>Mature_446_residues
TKVITRFAPSPTGMLHVGNIRVALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIKDDLKFLNLHWDQTFNQLSRLSR
YDEIKNLLLDKKRLYACYETPDELELKRKFQLSKGLPPMYDRASLNLTEEQTEKYIEQGRNPHYRFLVNHEPISWHDMIK
GEIQYDGKALSDPIVIRADGSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQIQMFEALNNTPTTFGHLSLIINKDEKI
SKRVGGFEITTLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLINQFEISSFSKSPTIYQPKDLERLNHKLLISLDFD
EVKDHLKEIDAEYIDENFWLSIRPNLQKLRDVKDWWEICHHTPNVESLNLDQEYLKQAAELLPQGAITKDSWSIWTKEIT
NITGRKGKELFLPLRLALTGRESGPEIADVLPLISREEIVKRLTSA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=486, Percent_Identity=25.9259259259259, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1788743, Length=339, Percent_Identity=37.7581120943953, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=29.9595141700405, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786895, Length=147, Percent_Identity=31.9727891156463, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=334, Percent_Identity=31.7365269461078, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17511007, Length=69, Percent_Identity=42.0289855072464, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=328, Percent_Identity=33.2317073170732, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321192, Length=236, Percent_Identity=29.6610169491525, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=146, Percent_Identity=32.1917808219178, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=27.6073619631902, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_RICAH (A8GN11)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493294.1
- ProteinModelPortal:   A8GN11
- SMR:   A8GN11
- STRING:   A8GN11
- GeneID:   5645148
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_02435
- NMPDR:   fig|293614.3.peg.452
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   RAKA293614:A1C_02435-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51874; Mature: 51743

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVITRFAPSPTGMLHVGNIRVALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIKD
CCCCEECCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
DLKFLNLHWDQTFNQLSRLSRYDEIKNLLLDKKRLYACYETPDELELKRKFQLSKGLPPM
HHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCC
YDRASLNLTEEQTEKYIEQGRNPHYRFLVNHEPISWHDMIKGEIQYDGKALSDPIVIRAD
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCEEEECCEECCCCEEEEEC
GSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQIQMFEALNNTPTTFGHLSLIINKDEK
CCHHHHHHHHHHCCCCCHHEEECCCCCCCCCEEEEEEEHHHCCCCCCEEEEEEEECCCHH
ISKRVGGFEITTLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLINQFEISSFSKSPT
HHHHCCCEEEEEHHHHHCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCCCC
IYQPKDLERLNHKLLISLDFDEVKDHLKEIDAEYIDENFWLSIRPNLQKLRDVKDWWEIC
CCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
HHTPNVESLNLDQEYLKQAAELLPQGAITKDSWSIWTKEITNITGRKGKELFLPLRLALT
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEE
GRESGPEIADVLPLISREEIVKRLTSA
CCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKVITRFAPSPTGMLHVGNIRVALLNWLYAKKHNGQFILRFDDTDLERSKQEYKDAIKD
CCCEECCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
DLKFLNLHWDQTFNQLSRLSRYDEIKNLLLDKKRLYACYETPDELELKRKFQLSKGLPPM
HHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCC
YDRASLNLTEEQTEKYIEQGRNPHYRFLVNHEPISWHDMIKGEIQYDGKALSDPIVIRAD
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCEEEECCEECCCCEEEEEC
GSMTYMLCSVIDDIDYDITHIIRGEDHVSNTAIQIQMFEALNNTPTTFGHLSLIINKDEK
CCHHHHHHHHHHCCCCCHHEEECCCCCCCCCEEEEEEEHHHCCCCCCEEEEEEEECCCHH
ISKRVGGFEITTLRKEIGIEAMAIASFFSLLGSSAQILPYKSMEKLINQFEISSFSKSPT
HHHHCCCEEEEEHHHHHCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCCCC
IYQPKDLERLNHKLLISLDFDEVKDHLKEIDAEYIDENFWLSIRPNLQKLRDVKDWWEIC
CCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
HHTPNVESLNLDQEYLKQAAELLPQGAITKDSWSIWTKEITNITGRKGKELFLPLRLALT
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEE
GRESGPEIADVLPLISREEIVKRLTSA
CCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA