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The map label for this gene is mnhA2 [H]

Identifier: 157825502

GI number: 157825502

Start: 378813

End: 380276

Strand: Reverse

Name: mnhA2 [H]

Synonym: A1C_02015

Alternate gene names: 157825502

Gene position: 380276-378813 (Counterclockwise)

Preceding gene: 157825504

Following gene: 157825501

Centisome position: 30.89

GC content: 31.08

Gene sequence:

>1464_bases
ATGATTACACAATTTACTACTCCGAGTCTTTTGATTCTATCAACTTTGCTAGTTTGTGCATTAAACTTAACCAGTCCGTA
TGCCACTAAAAAAGATAGTTTTATACGTAATTTTTTGCTTATTATTATCGCTATTTTTTTCTTCGGTAATATTTTAATTA
TAGACTTGTTGTTTTTACAAGGTATTAGAGCAGGCTTTGAATTTAATATATTCGGTAATTACTCTATAGGATTTCATCTT
GAACCCTCAGGGCTCATATTCTTGAGCTTAATAGGCTTTTTATGGATTTGTGCTGTACTTTATACTCCAAAATATCTTGC
TGTTAATAATATAGAACACTCTTCGAGATTCTTATTTTTCTTTAACTTAACTATTCTAATCGGTGTTTTAATTGCTTTAT
CTAGCAATTTATTCACGATGTTTATTTGCTACGAGCTTTTAACTATTTCTACAGCTTTTTTAATAGGACACACTAGAAAT
AAAATAATACTTAGCGGGTTATATAAATATATAAAAATTCTGATGATTTATGCCACAATATTATTTTTACCGGCAGTGAT
AATTATTTATACTAAAACCGGTAATGGGGATTTTGCAAGCGGCAGCTTAATAGAAAATTATTTTTCCAAAAACCAGTCAA
TTATTTTATTATTAATGTTTATTTTCGGTATTGCCAAAACTGCAATTTTTCCGGTGCATTCATGGCTTCCTGCCGCAATG
GTTGCACATTACCCCGTTAGTAGCTTACTGCATGCTGTAATAGTGGTAAAAACCGGATTATTTTGTGTTTACAAAATCCT
ATTATATATATTCGGTTTATCGTATTTACAAACGATATTTGCCGAGTTTAACTGGTTAATTTTCATACCAATAGTCAGCA
TATTTTATAGCTCACTCAAAGCACTAGGCACGGATAATATTAAAAAAATACTCGCCTACTCCACTATGAACCAATTAAGC
CTTGCCTTACTTAGTGCTTTCATGCTCACACCTAAGGCACTTGGAGCCGCAATACTACATTTAGTTTCACATTCTTTTAC
AAAAATCTGCTTATTTTATAGTATGGGAAGTATTTACAGTTTAAAGAAAACCGATCAAGTACAGAATTTACATGGCACAT
CCAAAGAATTGCCTTTAATTTCTTTTGTGATATTGATCTCTTCATTATCGATAATCGGTATACCTATCTTTAGCGGATTT
ATTAGCAAATTCTCAATCTTACTTGCCGCAAGCGAACAAAATCAAATTATCGTTATGATTGTCGTAATAGCTAGTAGTAT
ACTCTCAAGCATTTACCTTCTCAAAATATTAAGCTCTATTTATAAACCTTCTTTGGTAGAATCTAGCGTAACAAAACAAC
TCCCATACTCTATGCAAATCAGTATAATAATTTGCTGCTGTGCTGTAACTCTCTTCTATTTTATTCAAATCTTAATTAGA
GAATTTTTAGCTTACATTACATAA

Upstream 100 bases:

>100_bases
GATTGCTTTGTCAAAACTTACAGTTTTTCCTCGCAATGACAGGGATTTTCAAAACTGCCCGATACTATAAGGTCAAGCAA
ACTAGGTAACAAGGAAAAAC

Downstream 100 bases:

>100_bases
ATATGTATTTACATAATTGATGTATTGACATATAATAACATAGTATGCAATTTTTTTAGGATAAATATGGCACAAATTAT
TAGAGCAGCAGAATTTGTAC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A2; Putative NADH-ubiquinone oxidoreductase subunit mnhA2 [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MITQFTTPSLLILSTLLVCALNLTSPYATKKDSFIRNFLLIIIAIFFFGNILIIDLLFLQGIRAGFEFNIFGNYSIGFHL
EPSGLIFLSLIGFLWICAVLYTPKYLAVNNIEHSSRFLFFFNLTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTRN
KIILSGLYKYIKILMIYATILFLPAVIIIYTKTGNGDFASGSLIENYFSKNQSIILLLMFIFGIAKTAIFPVHSWLPAAM
VAHYPVSSLLHAVIVVKTGLFCVYKILLYIFGLSYLQTIFAEFNWLIFIPIVSIFYSSLKALGTDNIKKILAYSTMNQLS
LALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYSLKKTDQVQNLHGTSKELPLISFVILISSLSIIGIPIFSGF
ISKFSILLAASEQNQIIVMIVVIASSILSSIYLLKILSSIYKPSLVESSVTKQLPYSMQISIIICCCAVTLFYFIQILIR
EFLAYIT

Sequences:

>Translated_487_residues
MITQFTTPSLLILSTLLVCALNLTSPYATKKDSFIRNFLLIIIAIFFFGNILIIDLLFLQGIRAGFEFNIFGNYSIGFHL
EPSGLIFLSLIGFLWICAVLYTPKYLAVNNIEHSSRFLFFFNLTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTRN
KIILSGLYKYIKILMIYATILFLPAVIIIYTKTGNGDFASGSLIENYFSKNQSIILLLMFIFGIAKTAIFPVHSWLPAAM
VAHYPVSSLLHAVIVVKTGLFCVYKILLYIFGLSYLQTIFAEFNWLIFIPIVSIFYSSLKALGTDNIKKILAYSTMNQLS
LALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYSLKKTDQVQNLHGTSKELPLISFVILISSLSIIGIPIFSGF
ISKFSILLAASEQNQIIVMIVVIASSILSSIYLLKILSSIYKPSLVESSVTKQLPYSMQISIIICCCAVTLFYFIQILIR
EFLAYIT
>Mature_487_residues
MITQFTTPSLLILSTLLVCALNLTSPYATKKDSFIRNFLLIIIAIFFFGNILIIDLLFLQGIRAGFEFNIFGNYSIGFHL
EPSGLIFLSLIGFLWICAVLYTPKYLAVNNIEHSSRFLFFFNLTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTRN
KIILSGLYKYIKILMIYATILFLPAVIIIYTKTGNGDFASGSLIENYFSKNQSIILLLMFIFGIAKTAIFPVHSWLPAAM
VAHYPVSSLLHAVIVVKTGLFCVYKILLYIFGLSYLQTIFAEFNWLIFIPIVSIFYSSLKALGTDNIKKILAYSTMNQLS
LALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYSLKKTDQVQNLHGTSKELPLISFVILISSLSIIGIPIFSGF
ISKFSILLAASEQNQIIVMIVVIASSILSSIYLLKILSSIYKPSLVESSVTKQLPYSMQISIIICCCAVTLFYFIQILIR
EFLAYIT

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=331, Percent_Identity=29.0030211480363, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1788614, Length=378, Percent_Identity=27.5132275132275, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788827, Length=316, Percent_Identity=27.2151898734177, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=27.4509803921569, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788829, Length=392, Percent_Identity=27.0408163265306, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788613, Length=409, Percent_Identity=24.2053789731051, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693160, Length=345, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI2367154, Length=299, Percent_Identity=23.4113712374582, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54456; Mature: 54456

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQFTTPSLLILSTLLVCALNLTSPYATKKDSFIRNFLLIIIAIFFFGNILIIDLLFLQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRAGFEFNIFGNYSIGFHLEPSGLIFLSLIGFLWICAVLYTPKYLAVNNIEHSSRFLFF
HHHCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHH
FNLTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTRNKIILSGLYKYIKILMIYATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LFLPAVIIIYTKTGNGDFASGSLIENYFSKNQSIILLLMFIFGIAKTAIFPVHSWLPAAM
HHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAHYPVSSLLHAVIVVKTGLFCVYKILLYIFGLSYLQTIFAEFNWLIFIPIVSIFYSSLK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALGTDNIKKILAYSTMNQLSLALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LKKTDQVQNLHGTSKELPLISFVILISSLSIIGIPIFSGFISKFSILLAASEQNQIIVMI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHH
VVIASSILSSIYLLKILSSIYKPSLVESSVTKQLPYSMQISIIICCCAVTLFYFIQILIR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFLAYIT
HHHHHCC
>Mature Secondary Structure
MITQFTTPSLLILSTLLVCALNLTSPYATKKDSFIRNFLLIIIAIFFFGNILIIDLLFLQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRAGFEFNIFGNYSIGFHLEPSGLIFLSLIGFLWICAVLYTPKYLAVNNIEHSSRFLFF
HHHCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHH
FNLTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTRNKIILSGLYKYIKILMIYATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LFLPAVIIIYTKTGNGDFASGSLIENYFSKNQSIILLLMFIFGIAKTAIFPVHSWLPAAM
HHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAHYPVSSLLHAVIVVKTGLFCVYKILLYIFGLSYLQTIFAEFNWLIFIPIVSIFYSSLK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALGTDNIKKILAYSTMNQLSLALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LKKTDQVQNLHGTSKELPLISFVILISSLSIIGIPIFSGFISKFSILLAASEQNQIIVMI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHH
VVIASSILSSIYLLKILSSIYKPSLVESSVTKQLPYSMQISIIICCCAVTLFYFIQILIR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFLAYIT
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA