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The map label for this gene is queA

Identifier: 157825416

GI number: 157825416

Start: 293718

End: 294833

Strand: Reverse

Name: queA

Synonym: A1C_01585

Alternate gene names: 157825416

Gene position: 294833-293718 (Counterclockwise)

Preceding gene: 157825419

Following gene: 157825415

Centisome position: 23.95

GC content: 34.68

Gene sequence:

>1116_bases
ATGAAACTATCTGCTTTTGACTTTAATTTACCAAGCGAGCTTATAGCACAGTCTCCATCTAGTGAACGTGATAACTCAGA
TTTACTAATTGCGGCTATGCCGCCTATTAAAACTAAATTCTATAACATAATAGATTACTTAAAAGAAGGGGATTTATTAG
TCTTGAATAATAGCAAAGTGATAAAAGCAAAGCTACATTTGTGGAAGAATGTGACAATAAATTTGAATCAAAAATTATCG
GATGATAGTTGGTCGGCTTTTGCTAAACCGACTCGCAAGCTTCATGTAAATGATGAATTTTATTTTGATAATCATAAGGT
AATTATTACCGAAAAACTTGTGATGGGCGAGATTAAAGTAAAATTCGATCTTGATAATATTTCGGTATTTGAATTTTTGG
ACAAATACGGTGAGATGCCGCTGCCGGTTTATATTAGGAGGTCACATTCATTGTATCATACAACTAGCACTTTATGTCAT
ACCCGCGAAAACGGGTATTCAGAAAAATTAGATGCCTTCTTCCGCAGGAATGACATTAAAGATAAGGACAATGACATAGT
GTATGACAACGAACGCTATCAAACAATTTACAGTCAAATAGAAGGTTCAGTTGCAGCACCGACTGCAGGGTTGCACTTTA
CAAATGATATACTTGATAAACTCAAAACAAAAGGCATACATACTGCGTTTTTAACATTACATGTAGGAGCAGGAACTTTT
TTACCTGTAAAAACCGAAAATATTCATGAGCATAAAATGCACACTGAATATTGCTCTATTACTCCTGAAACTGCTGAAAT
CATCAACAAAGCAAAGCAAGAAAGGAGGCGTATTATCGCAGTCGGCACTACAACACTTAGAACAGTTGAGAGTTTTTGCA
ATAACGGCATCATAAAAGCAGGTAGCTTTGAAACCGATATCTTCATAACACCGGGTTTTAAATTTCAAACCGCAGATATG
TTACTTACTAATTTCCATTTCCCTAAATCTACTTTATTTATGCTTATATGTGCTTTTGCCGGCTTTAAAGAGATGCATGA
ATTATATAAATATGCTATAAAAGAGAAAATGCGTTTTTTCAGCTATGGCGATGCAACCCTTTTGTATAGGAAATGA

Upstream 100 bases:

>100_bases
AGTCTTCTATCAAAAATTCATGTCTTTAATAAACTAGAATCTTATATTAAATATTGTAATATATTGTTATAATGGCTTCT
TTAACTTCTAATATTCTATA

Downstream 100 bases:

>100_bases
TAATCACAATCGTCATTACGAGCAACTAAAATGATGGTGTAATCTAGAAAAAATAACAAAACACATGTTAAAGGTTAGCA
TTTTTAACTGGATTCCGTGA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLSAFDFNLPSELIAQSPSSERDNSDLLIAAMPPIKTKFYNIIDYLKEGDLLVLNNSKVIKAKLHLWKNVTINLNQKLS
DDSWSAFAKPTRKLHVNDEFYFDNHKVIITEKLVMGEIKVKFDLDNISVFEFLDKYGEMPLPVYIRRSHSLYHTTSTLCH
TRENGYSEKLDAFFRRNDIKDKDNDIVYDNERYQTIYSQIEGSVAAPTAGLHFTNDILDKLKTKGIHTAFLTLHVGAGTF
LPVKTENIHEHKMHTEYCSITPETAEIINKAKQERRRIIAVGTTTLRTVESFCNNGIIKAGSFETDIFITPGFKFQTADM
LLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEKMRFFSYGDATLLYRK

Sequences:

>Translated_371_residues
MKLSAFDFNLPSELIAQSPSSERDNSDLLIAAMPPIKTKFYNIIDYLKEGDLLVLNNSKVIKAKLHLWKNVTINLNQKLS
DDSWSAFAKPTRKLHVNDEFYFDNHKVIITEKLVMGEIKVKFDLDNISVFEFLDKYGEMPLPVYIRRSHSLYHTTSTLCH
TRENGYSEKLDAFFRRNDIKDKDNDIVYDNERYQTIYSQIEGSVAAPTAGLHFTNDILDKLKTKGIHTAFLTLHVGAGTF
LPVKTENIHEHKMHTEYCSITPETAEIINKAKQERRRIIAVGTTTLRTVESFCNNGIIKAGSFETDIFITPGFKFQTADM
LLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEKMRFFSYGDATLLYRK
>Mature_371_residues
MKLSAFDFNLPSELIAQSPSSERDNSDLLIAAMPPIKTKFYNIIDYLKEGDLLVLNNSKVIKAKLHLWKNVTINLNQKLS
DDSWSAFAKPTRKLHVNDEFYFDNHKVIITEKLVMGEIKVKFDLDNISVFEFLDKYGEMPLPVYIRRSHSLYHTTSTLCH
TRENGYSEKLDAFFRRNDIKDKDNDIVYDNERYQTIYSQIEGSVAAPTAGLHFTNDILDKLKTKGIHTAFLTLHVGAGTF
LPVKTENIHEHKMHTEYCSITPETAEIINKAKQERRRIIAVGTTTLRTVESFCNNGIIKAGSFETDIFITPGFKFQTADM
LLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEKMRFFSYGDATLLYRK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=380, Percent_Identity=37.8947368421053, Blast_Score=240, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_RICAH (A8GMK3)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493136.1
- ProteinModelPortal:   A8GMK3
- SMR:   A8GMK3
- STRING:   A8GMK3
- GeneID:   5645259
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_01585
- NMPDR:   fig|293614.3.peg.294
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK01424
- BioCyc:   RAKA293614:A1C_01585-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 42780; Mature: 42780

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSAFDFNLPSELIAQSPSSERDNSDLLIAAMPPIKTKFYNIIDYLKEGDLLVLNNSKV
CCCCEECCCCCHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCEE
IKAKLHLWKNVTINLNQKLSDDSWSAFAKPTRKLHVNDEFYFDNHKVIITEKLVMGEIKV
EEEEEEEEEEEEEEECCCCCCCCHHHHCCCCEEEEECCCEEECCCEEEEEEEEEEEEEEE
KFDLDNISVFEFLDKYGEMPLPVYIRRSHSLYHTTSTLCHTRENGYSEKLDAFFRRNDIK
EEECCCCHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHCCCCCCHHHHHHHHHHCCCC
DKDNDIVYDNERYQTIYSQIEGSVAAPTAGLHFTNDILDKLKTKGIHTAFLTLHVGAGTF
CCCCCEEECCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEEEEECCCEE
LPVKTENIHEHKMHTEYCSITPETAEIINKAKQERRRIIAVGTTTLRTVESFCNNGIIKA
EEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCEEC
GSFETDIFITPGFKFQTADMLLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEKMRFF
CCCCCEEEECCCCCEEHHHHHEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
SYGDATLLYRK
CCCCEEEEEEC
>Mature Secondary Structure
MKLSAFDFNLPSELIAQSPSSERDNSDLLIAAMPPIKTKFYNIIDYLKEGDLLVLNNSKV
CCCCEECCCCCHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCEE
IKAKLHLWKNVTINLNQKLSDDSWSAFAKPTRKLHVNDEFYFDNHKVIITEKLVMGEIKV
EEEEEEEEEEEEEEECCCCCCCCHHHHCCCCEEEEECCCEEECCCEEEEEEEEEEEEEEE
KFDLDNISVFEFLDKYGEMPLPVYIRRSHSLYHTTSTLCHTRENGYSEKLDAFFRRNDIK
EEECCCCHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHCCCCCCHHHHHHHHHHCCCC
DKDNDIVYDNERYQTIYSQIEGSVAAPTAGLHFTNDILDKLKTKGIHTAFLTLHVGAGTF
CCCCCEEECCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEEEEECCCEE
LPVKTENIHEHKMHTEYCSITPETAEIINKAKQERRRIIAVGTTTLRTVESFCNNGIIKA
EEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCEEC
GSFETDIFITPGFKFQTADMLLTNFHFPKSTLFMLICAFAGFKEMHELYKYAIKEKMRFF
CCCCCEEEECCCCCEEHHHHHEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
SYGDATLLYRK
CCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA