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The map label for this gene is sucB [H]

Identifier: 157825365

GI number: 157825365

Start: 240318

End: 241520

Strand: Reverse

Name: sucB [H]

Synonym: A1C_01295

Alternate gene names: 157825365

Gene position: 241520-240318 (Counterclockwise)

Preceding gene: 157825366

Following gene: 157825364

Centisome position: 19.62

GC content: 36.24

Gene sequence:

>1203_bases
ATGAGCGTTAAAATTATAGTACCGTTGCTTGGAGAGTCCGTAACAGAAGCAACTATTGCCAAATGGTATAAGAAAGAAGG
TGATTCGGTTAAAACCGATGAATTACTGTTAGAAATTGAAACCGAAAAAGTGACTTTAGAAGTTAATGCTCCTTGTAACG
GTACTATAGGTAAAATATCGAAAACTGATGGTGCAAATGTAGCAGTTGGAGAAGAAATAGGAGATATAAATGAAGGTGCA
GCTGTTAATACTGCCGGTACTCATACTGAATCTGCTAAAGCTCAAGAAGTTACGCAACCTACTTCAGAAAAGCCTGTAGA
CAGACCTGCCATGGTTAATAATATTCTTGCTCCTTCCGTACAAAAATTAGTTACCGAAAATAAGCTTGACCCCAATAATA
TAAAAGGAACAGGTAAAGATGCTAGAATTACCAAAAGCGACGTGCTTGAAACAATAAACACAAAGTCTGCTGCTACTTCT
ACTACAGTAAACAAAACTAACGAAGAAAGAGTACAGCGTGTTCGTATGTCACGCTTACGTAAAACTATTGCACAGCGTTT
AAAAGATTCACAAAATACAGCAGCTATTTTGACTACTTTTAATGAAATCGATATGTCAAAAGTAATTGCATTGCGTAATC
AATATAAGGAAGAGTTCGAGAAAAAGCACGATGTGAAACTTGGCTTTATGTCGTTTTTTGTTAAAGCAACAATTGAAGCT
TTAAAGCTTATTCCATCGGTCAATGCTGAAATAGATGGTGATGATTTAGTATATAAAAATTACTATGATATAGGTGTAGC
TGTCGGAACAGAGCAAGGACTTGTTGTACCGGTTGTTAGGGATGCCGATCAAATGGGATTTGCTGAGGTAGAAAAAGCTA
TAGGAACTTTGGCTAAAAAAGCTCGTGAGGGTAAACTTTCTATGGCTGATTTGTCAGGGGGAACATTTTCGATTTCTAAC
GGAGGCGTATATGGCTCATTATTATCTACACCGATTATTAATCCTCCTCAATCAGGTATTTTAGGATTACATAAAACTGA
GGAAAGAGCTGTAGTTATAGACGGTAAAATTGAAATACATCCGATGATGTATATAGCTTTATCTTACGATCATCGTATAA
TTGACGGAAAAGAAGGAGTATCGTTCTTGGTAAAAATTAAGCAGCTTATTGAGAACCCTGAGAAGTTACTACTGAATTTG
TAA

Upstream 100 bases:

>100_bases
TAAAAACGTCTGTTTCACCCCGTGGGTTTTACCGATCCATGCAACAATCCACGGGATAATACCGAATACGTTTTTCGATT
TTTTAGGAGTAAAATAAATT

Downstream 100 bases:

>100_bases
AAAATTGTCATGCCGTGGTCAAGCCATAGGGTGCCACCCCAAAGCCTTGAATATCCGCCTACGCGGGATTGACATAAATG
AGAAACATACGGTATCGGTG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSVKIIVPLLGESVTEATIAKWYKKEGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKISKTDGANVAVGEEIGDINEGA
AVNTAGTHTESAKAQEVTQPTSEKPVDRPAMVNNILAPSVQKLVTENKLDPNNIKGTGKDARITKSDVLETINTKSAATS
TTVNKTNEERVQRVRMSRLRKTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHDVKLGFMSFFVKATIEA
LKLIPSVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADQMGFAEVEKAIGTLAKKAREGKLSMADLSGGTFSISN
GGVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIHPMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL

Sequences:

>Translated_400_residues
MSVKIIVPLLGESVTEATIAKWYKKEGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKISKTDGANVAVGEEIGDINEGA
AVNTAGTHTESAKAQEVTQPTSEKPVDRPAMVNNILAPSVQKLVTENKLDPNNIKGTGKDARITKSDVLETINTKSAATS
TTVNKTNEERVQRVRMSRLRKTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHDVKLGFMSFFVKATIEA
LKLIPSVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADQMGFAEVEKAIGTLAKKAREGKLSMADLSGGTFSISN
GGVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIHPMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL
>Mature_399_residues
SVKIIVPLLGESVTEATIAKWYKKEGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKISKTDGANVAVGEEIGDINEGAA
VNTAGTHTESAKAQEVTQPTSEKPVDRPAMVNNILAPSVQKLVTENKLDPNNIKGTGKDARITKSDVLETINTKSAATST
TVNKTNEERVQRVRMSRLRKTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHDVKLGFMSFFVKATIEAL
KLIPSVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADQMGFAEVEKAIGTLAKKAREGKLSMADLSGGTFSISNG
GVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIHPMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=58.008658008658, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=29.6296296296296, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=27.9735682819383, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=27.9735682819383, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI110671329, Length=437, Percent_Identity=28.3752860411899, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=35.625, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1786946, Length=406, Percent_Identity=48.2758620689655, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1786305, Length=408, Percent_Identity=31.3725490196078, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=43.1761786600496, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=30.8045977011494, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=414, Percent_Identity=27.7777777777778, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=29.8360655737705, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=407, Percent_Identity=44.2260442260442, Blast_Score=322, Evalue=6e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=27.9642058165548, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=56.7685589519651, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=30, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=31.1688311688312, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=31.1688311688312, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43536; Mature: 43405

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKIIVPLLGESVTEATIAKWYKKEGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKIS
CCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCC
KTDGANVAVGEEIGDINEGAAVNTAGTHTESAKAQEVTQPTSEKPVDRPAMVNNILAPSV
CCCCCCEEECCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHH
QKLVTENKLDPNNIKGTGKDARITKSDVLETINTKSAATSTTVNKTNEERVQRVRMSRLR
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHDVKLGFMSFFVKATIEA
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKLIPSVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADQMGFAEVEKAIGTLAKK
HHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
AREGKLSMADLSGGTFSISNGGVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIH
HHCCCCEEEECCCCEEEECCCCEEHHHHCCCCCCCCCCCCCCCEECCCCEEEEECEEEEE
PMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL
EEEEEEEECCCEEECCCCHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SVKIIVPLLGESVTEATIAKWYKKEGDSVKTDELLLEIETEKVTLEVNAPCNGTIGKIS
CEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCC
KTDGANVAVGEEIGDINEGAAVNTAGTHTESAKAQEVTQPTSEKPVDRPAMVNNILAPSV
CCCCCCEEECCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHH
QKLVTENKLDPNNIKGTGKDARITKSDVLETINTKSAATSTTVNKTNEERVQRVRMSRLR
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KTIAQRLKDSQNTAAILTTFNEIDMSKVIALRNQYKEEFEKKHDVKLGFMSFFVKATIEA
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKLIPSVNAEIDGDDLVYKNYYDIGVAVGTEQGLVVPVVRDADQMGFAEVEKAIGTLAKK
HHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
AREGKLSMADLSGGTFSISNGGVYGSLLSTPIINPPQSGILGLHKTEERAVVIDGKIEIH
HHCCCCEEEECCCCEEEECCCCEEHHHHCCCCCCCCCCCCCCCEECCCCEEEEECEEEEE
PMMYIALSYDHRIIDGKEGVSFLVKIKQLIENPEKLLLNL
EEEEEEEECCCEEECCCCHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA