Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 157825339

GI number: 157825339

Start: 212060

End: 213619

Strand: Reverse

Name: emrB [H]

Synonym: A1C_01105

Alternate gene names: 157825339

Gene position: 213619-212060 (Counterclockwise)

Preceding gene: 157825342

Following gene: 157825338

Centisome position: 17.35

GC content: 34.23

Gene sequence:

>1560_bases
ATGTCAAATGATAATTTACAACCACCTGCCCTTTCTAAAAAGCAATTGTTTGCTTTTTTTGGTATGGTTGTCGGAATGTT
CATGTCGGTACTTGATATTCAAATTGTCGCTAGCTCATTATCTGTAATAGCTGCAGGTCTTGCCGCTTCAAGCGATGAAC
TTTCTTGGGTTCAAACATCTTACTTAATAGCTGAAGTTATCATTATTCCTATTACCGGCTTTTTAGCAAGGTTACTTTCT
ACTAGAGTTTCTTATTTCATTGCAGCTTTAGGGTTTACTGTTATGAGCGTGTTATGTGCGCTTGCAACTAATATTGAATC
CATTATTATCTTTAGAGCATTACAAGGATTTTTTGGCGGTGCTATGATACCAACGGTCTTTAGTACCGTTTTTATAATTT
TTCCTGCATCACAACGTCCTACTGTTACTATCTTAATTGGACTTGTCGTAACTGTTGCCCCTACTTTAGGACCTACACTC
GGTGGATATATTACAGAGATTTTATCATGGCATTTTATGTTTGTACTAAACGTCATACCCGGTATTTTTGTATGCACGGT
AGTATTTTTATATGGGGATTTTGATAAACCAAATTATAAATTACTGAAAAATTTTGATTTTCTCGGTATATTATTAATGG
CTTTAACGCTCGGCTTATTACAATATGTTTTAGAAGAAGGAAATAAAAAAGGTTGGCTTGAGGATAATTTAATATTATTC
TTAAGTATTGCAGTAGCTTTAGGTTTTATTCTATTAATCATTAGAGAACTAACCTGTATTAATCCAATTTTAGATTTAAA
AACATTCCTTGATAAAGACTTTACTTTTGGTTGCCTTTATTCATTCGTTATGGGTATTGGATTATACGGGGCAGTATATA
TATTGCCGCTATTTCTCTTTACTATTGCCGGATATAATACATTACAAATCGGGGCTACTATGATGGTAACGGGCGGAGCA
CAATTTTTATCTGCACCACTTGCCGGTAGGATGTTAGGGCTAGGGGTAGATTTACGCCTGATGCTTATTATAGGGCTTGG
AGGGTTTGCTCTTGGATGCCATTTGAATAGTTTTCTAACACCTGATTCTAAATTTGCTGCCTTTGTGCTGCCTCAAGTTG
TTAGAGGGCTTTCCTTAATGTTTTGTTTTATACCGACTAATAATGTAGCGCTTGGCAATATGCCAAAAGAACGAGTAGGT
AACGCTAGCGGGCTTTATAATCTTACTCGTAATTTAGGAGGAGCGGTAGGGCTTGCAATAATCAGCACTATACTTACTAA
TGATACTAAAATCTTTATGCAATATTTATCGGAAAATATCTCGTCTACTTCTATAATTGCATTAGAATACCTAGATTCTT
ATACTGAATTATTAAGCGGAAAAGCGCTGCATCCTGACACAGCATCATATTTGTTATTAGCAAACAAATTAAATAATGAT
GCTTTTGTAATTGCAATAAATAATATATTTAATATGATAGGACTGTTATTTGTATTTATAATGCTACTAATACCTTTTAC
TTCAAATATCAAATTAACCGGCAATGTTAATGCTCACTAA

Upstream 100 bases:

>100_bases
TTAGATTAAAAATTAAAAGTTGTACAACAATTTTTTATTTTAAACATATATTTTGATGTCACTTATTAACTTTATTTAAT
GCAAACTGTATAATTTAATT

Downstream 100 bases:

>100_bases
AATTAAATTTATATTATATTTTCCCTGGTTATTATTAGAAATATGGAAATCAGCTTTCTCGGTTATCAAAATAATTTGGC
AAAGAAAGATAGAGATAGAG

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSNDNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLS
TRVSYFIAALGFTVMSVLCALATNIESIIIFRALQGFFGGAMIPTVFSTVFIIFPASQRPTVTILIGLVVTVAPTLGPTL
GGYITEILSWHFMFVLNVIPGIFVCTVVFLYGDFDKPNYKLLKNFDFLGILLMALTLGLLQYVLEEGNKKGWLEDNLILF
LSIAVALGFILLIIRELTCINPILDLKTFLDKDFTFGCLYSFVMGIGLYGAVYILPLFLFTIAGYNTLQIGATMMVTGGA
QFLSAPLAGRMLGLGVDLRLMLIIGLGGFALGCHLNSFLTPDSKFAAFVLPQVVRGLSLMFCFIPTNNVALGNMPKERVG
NASGLYNLTRNLGGAVGLAIISTILTNDTKIFMQYLSENISSTSIIALEYLDSYTELLSGKALHPDTASYLLLANKLNND
AFVIAINNIFNMIGLLFVFIMLLIPFTSNIKLTGNVNAH

Sequences:

>Translated_519_residues
MSNDNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLS
TRVSYFIAALGFTVMSVLCALATNIESIIIFRALQGFFGGAMIPTVFSTVFIIFPASQRPTVTILIGLVVTVAPTLGPTL
GGYITEILSWHFMFVLNVIPGIFVCTVVFLYGDFDKPNYKLLKNFDFLGILLMALTLGLLQYVLEEGNKKGWLEDNLILF
LSIAVALGFILLIIRELTCINPILDLKTFLDKDFTFGCLYSFVMGIGLYGAVYILPLFLFTIAGYNTLQIGATMMVTGGA
QFLSAPLAGRMLGLGVDLRLMLIIGLGGFALGCHLNSFLTPDSKFAAFVLPQVVRGLSLMFCFIPTNNVALGNMPKERVG
NASGLYNLTRNLGGAVGLAIISTILTNDTKIFMQYLSENISSTSIIALEYLDSYTELLSGKALHPDTASYLLLANKLNND
AFVIAINNIFNMIGLLFVFIMLLIPFTSNIKLTGNVNAH
>Mature_518_residues
SNDNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLST
RVSYFIAALGFTVMSVLCALATNIESIIIFRALQGFFGGAMIPTVFSTVFIIFPASQRPTVTILIGLVVTVAPTLGPTLG
GYITEILSWHFMFVLNVIPGIFVCTVVFLYGDFDKPNYKLLKNFDFLGILLMALTLGLLQYVLEEGNKKGWLEDNLILFL
SIAVALGFILLIIRELTCINPILDLKTFLDKDFTFGCLYSFVMGIGLYGAVYILPLFLFTIAGYNTLQIGATMMVTGGAQ
FLSAPLAGRMLGLGVDLRLMLIIGLGGFALGCHLNSFLTPDSKFAAFVLPQVVRGLSLMFCFIPTNNVALGNMPKERVGN
ASGLYNLTRNLGGAVGLAIISTILTNDTKIFMQYLSENISSTSIIALEYLDSYTELLSGKALHPDTASYLLLANKLNNDA
FVIAINNIFNMIGLLFVFIMLLIPFTSNIKLTGNVNAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=503, Percent_Identity=28.0318091451292, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=29.7405189620758, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1790195, Length=419, Percent_Identity=25.5369928400955, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=27.0983213429257, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=308, Percent_Identity=30.1948051948052, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322958, Length=279, Percent_Identity=26.5232974910394, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56322; Mature: 56191

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
SNDNLQPPALSKKQLFAFFGMVVGMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH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EEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]