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The map label for this gene is asnA

Identifier: 157373136

GI number: 157373136

Start: 5443601

End: 5444593

Strand: Reverse

Name: asnA

Synonym: Spro_4905

Alternate gene names: 157373136

Gene position: 5444593-5443601 (Counterclockwise)

Preceding gene: NA

Following gene: 157373133

Centisome position: 99.92

GC content: 56.19

Gene sequence:

>993_bases
ATGAAAAAACAGTTTATTCAAAAACAACAACAAATCAGCCTGGTTAAATCCTTTTTCTCCCGCCAGTTGGAGCAACAGCT
TGGCCTGATCGAAGTGCAGGCGCCGATCCTCAGCCGTCTGGGTGACGGCACCCAGGACAACCTGTCCGGTAGCGAGAAAG
CGGTACAGGTAAAGGTGAAAACCCTGCCGGACGCCACCTTCGAAGTGGTGCACTCACTGGCCAAATGGAAGCGTAAAACG
CTGGGCAGCTATGACTTTGGCGCCGGTGAAGGCCTGTATACCCATATGAAAGCACTGCGTCCGGACGAAGATCGTCTGAG
CGCCATCCATTCGGTTTACGTTGATCAATGGGACTGGGAGCGGGTGATGGGCGATGGCGAACGCAGCCAGGATTACCTCG
AAAGCACCGTGCGCAGTATTTATGCGGCGATTAAGGCTACCGAAGGGGAAGTGAGTCGCGAATACGGCTTAACGCCGTTC
CTGCCGGAGCAAATCCATTTTGTGCACAGCGAAACCCTGCTGCAACGCTACCCTGAACTGGATGCCAAAGGCCGTGAGCG
GGCGATTGCTAAAGAGCTGGGCGCGGTATTTCTGATCGGTATTGGTGGCAAATTGTCACACGGCAAATCACACGATGTGC
GTGCGCCGGATTATGATGACTGGACCACCCCAGCAGCTGAAGGGTTGGCCGGCCTGAACGGTGACATCCTGGTATGGAAC
CCGGTGCTGCAGGACGCCTTCGAACTTTCTTCTATGGGGATCCGCGTTGACGCCAGCGCGCTGAAACGCCAATTGGCACA
GACCGGTGATGAAGATCGCCTGGCGCTGGAATGGCATCAGTCATTGCTGCGTGGCGAGATGCCACAAACCATCGGTGGCG
GTATCGGTCAGTCGCGCCTGGTGATGCTATTGCTGCAACTGTCGCACATCGGGCAGGTACAGTGCGGCGTGTGGTCACCT
CAGGTACGTGAGGCGGTAGAAGGGCTGCTGTAA

Upstream 100 bases:

>100_bases
TAAAAGTGAAAAGGCAATATTGATTAGCGGATATTGAATTGTGGTGCCGTTCTGTTGCTTAATCGTTGGCAACAGAGACA
GGTTTATAAGAGATACGGCA

Downstream 100 bases:

>100_bases
TACTCAGCGCCGCCAGCGGCGCATTAAGCGGCTTTTTAACCCGGTATCAAAGCGCCAGATATGATCGAAGATACGCATGA
TACCGGGCTTGCCGTAGTTG

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKQFIQKQQQISLVKSFFSRQLEQQLGLIEVQAPILSRLGDGTQDNLSGSEKAVQVKVKTLPDATFEVVHSLAKWKRKT
LGSYDFGAGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWERVMGDGERSQDYLESTVRSIYAAIKATEGEVSREYGLTPF
LPEQIHFVHSETLLQRYPELDAKGRERAIAKELGAVFLIGIGGKLSHGKSHDVRAPDYDDWTTPAAEGLAGLNGDILVWN
PVLQDAFELSSMGIRVDASALKRQLAQTGDEDRLALEWHQSLLRGEMPQTIGGGIGQSRLVMLLLQLSHIGQVQCGVWSP
QVREAVEGLL

Sequences:

>Translated_330_residues
MKKQFIQKQQQISLVKSFFSRQLEQQLGLIEVQAPILSRLGDGTQDNLSGSEKAVQVKVKTLPDATFEVVHSLAKWKRKT
LGSYDFGAGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWERVMGDGERSQDYLESTVRSIYAAIKATEGEVSREYGLTPF
LPEQIHFVHSETLLQRYPELDAKGRERAIAKELGAVFLIGIGGKLSHGKSHDVRAPDYDDWTTPAAEGLAGLNGDILVWN
PVLQDAFELSSMGIRVDASALKRQLAQTGDEDRLALEWHQSLLRGEMPQTIGGGIGQSRLVMLLLQLSHIGQVQCGVWSP
QVREAVEGLL
>Mature_330_residues
MKKQFIQKQQQISLVKSFFSRQLEQQLGLIEVQAPILSRLGDGTQDNLSGSEKAVQVKVKTLPDATFEVVHSLAKWKRKT
LGSYDFGAGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWERVMGDGERSQDYLESTVRSIYAAIKATEGEVSREYGLTPF
LPEQIHFVHSETLLQRYPELDAKGRERAIAKELGAVFLIGIGGKLSHGKSHDVRAPDYDDWTTPAAEGLAGLNGDILVWN
PVLQDAFELSSMGIRVDASALKRQLAQTGDEDRLALEWHQSLLRGEMPQTIGGGIGQSRLVMLLLQLSHIGQVQCGVWSP
QVREAVEGLL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=330, Percent_Identity=80.9090909090909, Blast_Score=547, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_SERP5 (A8GLK7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481125.1
- ProteinModelPortal:   A8GLK7
- SMR:   A8GLK7
- STRING:   A8GLK7
- GeneID:   5603667
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4905
- eggNOG:   COG2502
- HOGENOM:   HBG288146
- OMA:   LNDNLNG
- ProtClustDB:   PRK05425
- BioCyc:   SPRO399741:SPRO_4905-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 36733; Mature: 36733

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQFIQKQQQISLVKSFFSRQLEQQLGLIEVQAPILSRLGDGTQDNLSGSEKAVQVKVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHCCCCCCCCCCCCCCEEEEEEE
TLPDATFEVVHSLAKWKRKTLGSYDFGAGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
RVMGDGERSQDYLESTVRSIYAAIKATEGEVSREYGLTPFLPEQIHFVHSETLLQRYPEL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
DAKGRERAIAKELGAVFLIGIGGKLSHGKSHDVRAPDYDDWTTPAAEGLAGLNGDILVWN
CCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEC
PVLQDAFELSSMGIRVDASALKRQLAQTGDEDRLALEWHQSLLRGEMPQTIGGGIGQSRL
HHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHH
VMLLLQLSHIGQVQCGVWSPQVREAVEGLL
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKKQFIQKQQQISLVKSFFSRQLEQQLGLIEVQAPILSRLGDGTQDNLSGSEKAVQVKVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHCCCCCCCCCCCCCCEEEEEEE
TLPDATFEVVHSLAKWKRKTLGSYDFGAGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
RVMGDGERSQDYLESTVRSIYAAIKATEGEVSREYGLTPFLPEQIHFVHSETLLQRYPEL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
DAKGRERAIAKELGAVFLIGIGGKLSHGKSHDVRAPDYDDWTTPAAEGLAGLNGDILVWN
CCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEC
PVLQDAFELSSMGIRVDASALKRQLAQTGDEDRLALEWHQSLLRGEMPQTIGGGIGQSRL
HHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHH
VMLLLQLSHIGQVQCGVWSPQVREAVEGLL
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA