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The map label for this gene is murI

Identifier: 157373001

GI number: 157373001

Start: 5282532

End: 5283395

Strand: Reverse

Name: murI

Synonym: Spro_4769

Alternate gene names: 157373001

Gene position: 5283395-5282532 (Counterclockwise)

Preceding gene: 157373002

Following gene: 157373000

Centisome position: 96.96

GC content: 53.36

Gene sequence:

>864_bases
ATGGCTACGCTACTCCAGGAAGAGAATACTACCTCACTGGAAGCTATACCTTCTAACACCACCGCGAAGACCCGTCCGAC
GGTACTGGTATTTGATTCCGGCGTCGGCGGGTTATCGGTGTATCAAGAGGTTCGGCAACTGTTGCCGGACCTGCACTATA
TATACGCTTTCGATAATGTGGCGTTTCCCTACGGTGAGAAATCCGAAGAGTTTATTGTTGAACGCGTGCTGGAGATTGTC
AGCGCGGTGCAGCAGCGCCATCCTCTGGCGATAGTCATCATCGCTTGTAACACCGCCAGTACCGTATCCTTACCGGCCTT
ACGTGAACGCTTTACCTTCCCGGTCGTTGGTGTGGTACCTGCCATCAAACCTGCCGCCCGTCTGACCGCCAATGGCGTTG
TCGGCCTGCTGGCTACCCGTGGTACCGTACAGCGCACCTATACTCATGAGTTGATTGCCCGCTTCGCCACCGATTGCAAA
ATTGAGCTATTGGGGTCCTCAGAGCTGGTGGAACTGGCAGAAGCCAAGCTGCATGGCGAAGCTGTACCCTTGCCAACGCT
GAAGAAAATCCTGCATCCATGGCTGAGCATGCGTGAGCCGCCGGATACCGTAGTTCTCGGTTGCACCCACTTCCCTTTAT
TAGCAGAAGAGTTAATGCAGGTGTTGCCTGAGGGAACCCGGCTGATCGATTCCGGCGCGGCAATCGCCCGTCGTACCGCC
TGGTTGATCTCGACACAGGAAAATCTGGTGTCTACGCAGGAAGATAATCTGGCGTATTGCATGGCGTTAAATGAAGACAC
TGACGCTTTATTGCCCGTTCTGCAGGGATATGGCTTTAAATCGTTGAAAAAACTGCCTGTTTAA

Upstream 100 bases:

>100_bases
GTCAGCCTGTGGGATCTCGCAGTTTCGTATCCGGTCACATCTCATCTGACAGTTCGTGGTAGAATCGCCAACCTGTTTGA
TAAAGATTATGAGACGGCTT

Downstream 100 bases:

>100_bases
GAGCGTTTTGGTTAAACATTCAACGGTCGGAAAAAATATTGAAATTAGGGGTTGCGGCGTTCTGAGAACTCCCTATAATG
CGCCTCCACTGACCGGGAAC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MATLLQEENTTSLEAIPSNTTAKTRPTVLVFDSGVGGLSVYQEVRQLLPDLHYIYAFDNVAFPYGEKSEEFIVERVLEIV
SAVQQRHPLAIVIIACNTASTVSLPALRERFTFPVVGVVPAIKPAARLTANGVVGLLATRGTVQRTYTHELIARFATDCK
IELLGSSELVELAEAKLHGEAVPLPTLKKILHPWLSMREPPDTVVLGCTHFPLLAEELMQVLPEGTRLIDSGAAIARRTA
WLISTQENLVSTQEDNLAYCMALNEDTDALLPVLQGYGFKSLKKLPV

Sequences:

>Translated_287_residues
MATLLQEENTTSLEAIPSNTTAKTRPTVLVFDSGVGGLSVYQEVRQLLPDLHYIYAFDNVAFPYGEKSEEFIVERVLEIV
SAVQQRHPLAIVIIACNTASTVSLPALRERFTFPVVGVVPAIKPAARLTANGVVGLLATRGTVQRTYTHELIARFATDCK
IELLGSSELVELAEAKLHGEAVPLPTLKKILHPWLSMREPPDTVVLGCTHFPLLAEELMQVLPEGTRLIDSGAAIARRTA
WLISTQENLVSTQEDNLAYCMALNEDTDALLPVLQGYGFKSLKKLPV
>Mature_286_residues
ATLLQEENTTSLEAIPSNTTAKTRPTVLVFDSGVGGLSVYQEVRQLLPDLHYIYAFDNVAFPYGEKSEEFIVERVLEIVS
AVQQRHPLAIVIIACNTASTVSLPALRERFTFPVVGVVPAIKPAARLTANGVVGLLATRGTVQRTYTHELIARFATDCKI
ELLGSSELVELAEAKLHGEAVPLPTLKKILHPWLSMREPPDTVVLGCTHFPLLAEELMQVLPEGTRLIDSGAAIARRTAW
LISTQENLVSTQEDNLAYCMALNEDTDALLPVLQGYGFKSLKKLPV

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=287, Percent_Identity=73.8675958188153, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_SERP5 (A8GL72)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480990.1
- ProteinModelPortal:   A8GL72
- SMR:   A8GL72
- STRING:   A8GL72
- GeneID:   5602457
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4769
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- BioCyc:   SPRO399741:SPRO_4769-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 31400; Mature: 31269

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLLQEENTTSLEAIPSNTTAKTRPTVLVFDSGVGGLSVYQEVRQLLPDLHYIYAFDNV
CCCCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHEEEEEECCE
AFPYGEKSEEFIVERVLEIVSAVQQRHPLAIVIIACNTASTVSLPALRERFTFPVVGVVP
ECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHH
AIKPAARLTANGVVGLLATRGTVQRTYTHELIARFATDCKIELLGSSELVELAEAKLHGE
CCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCC
AVPLPTLKKILHPWLSMREPPDTVVLGCTHFPLLAEELMQVLPEGTRLIDSGAAIARRTA
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEHHCCHHHHHHHH
WLISTQENLVSTQEDNLAYCMALNEDTDALLPVLQGYGFKSLKKLPV
HEEECHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
ATLLQEENTTSLEAIPSNTTAKTRPTVLVFDSGVGGLSVYQEVRQLLPDLHYIYAFDNV
CCCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHEEEEEECCE
AFPYGEKSEEFIVERVLEIVSAVQQRHPLAIVIIACNTASTVSLPALRERFTFPVVGVVP
ECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHH
AIKPAARLTANGVVGLLATRGTVQRTYTHELIARFATDCKIELLGSSELVELAEAKLHGE
CCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCC
AVPLPTLKKILHPWLSMREPPDTVVLGCTHFPLLAEELMQVLPEGTRLIDSGAAIARRTA
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEHHCCHHHHHHHH
WLISTQENLVSTQEDNLAYCMALNEDTDALLPVLQGYGFKSLKKLPV
HEEECHHHHHCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA