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The map label for this gene is fadH [C]

Identifier: 157372917

GI number: 157372917

Start: 5175357

End: 5177120

Strand: Reverse

Name: fadH [C]

Synonym: Spro_4685

Alternate gene names: 157372917

Gene position: 5177120-5175357 (Counterclockwise)

Preceding gene: 157372918

Following gene: 157372915

Centisome position: 95.01

GC content: 49.21

Gene sequence:

>1764_bases
ATGATCATTACGGAATATTACGCCATAGCGCCTCATGTCGGTTTTTTCCCCCGGGCGTTAGGGATTTGGAATGATGATCT
GATAAAAAATCATAGTGAATTGACTCGGCGAGTACACGGTGCCAGGGCGAAAATTGCCGCACAGATATCCCATGCCGGCA
GGGAGATTTATCGCGATCACCCAAGAGAAAATGCCGTCAGTTCCTCGGCGCTGAAAGACGTCTACGGCGAGTTCAGACCG
CGAGAAATAACCCTGCCAGAAATCAAGAAAATCGTCTCGCAGTATGGCGATACGGCCAAAAATCTTAAAAAGGCCAATTT
TGACGCGGTGGAAATTTATGCGGCGCATGGTTTCCTTATTTCAAATTTTTTATCGAAATATACTAACCATAGAACTGACG
AGTATGGCGGCAGTTTGAGAGATCGCATGCGGTTTCTGGAGGAGATCGTTAAGGACGTCAGGAGCAAAGTGGGGGCAGAT
TTTCCCGTATTGGTAAGACTATCCACCAAGGAGTTCGTGCCTGGGGGACTATCGCTGGCAGAGACCCGGGTCATCGTCAA
AAGGATGGAAGAGCTCGGCATAGATGCCATTAATTGCTCTCAAGGCATTGTCACCGTCTCCAATAATATCGTTGAACCCT
TCCAGGTGGGGAATGCGCCTTTTGTGGATATTGCTGAGGAAATCAAGAAAATCGTCAGCATCCCGGTGATCACTACCGGG
CGAATTAACGATGCGGATCTTGCTGAAACAACCCTATTGGCAGGGAAAAGTGATTTTATTGGCATGGCGCGGGCCTCACT
TGCGGATCCTGATCTGCCGAAAAAAGTGTTGGCAGGGCGCTGGGATGAAATTCGGCACTGTATCGGCTGTAATCAGGGAT
GTATCGGTAACAACATGCGCGGGGAGGTTTGCCGTTGTATGGTTAATCCGGAGATCAATCGCGAATATCTGCTGGATCTG
AATCCCGCCGTAGAGAAAAAGAACGTCACCATTATTGGCGGAGGCATTGCCGGTTGTGAAGCCGCAATTGCCGCCGCACG
CAAAGGCCATAATGTCACCTTGCATGAAAAATCAGATCGGCTGGGGGGCACCTGGTTAATAGCCAGCTTGCCTTTATCTA
AAGGGGAATTGGTTTCTCTGGTTAAATGGCAAATCAGTGAAATGAAACGGCTGAATATTCATCTATGCGTTAATTCTTCC
CCTACGGTGGAGGAAATTGTGGCGTCTTGCCCGGATAAGGTAATTATTGCTACCGGCAGCCAGCCTATTTTACCGCCGAT
TAAAGGGATTGAGAGTAAAATTGTCGTGCAGGCCAATAAAATATTAACGCAGGATGCGCCATTCGGGGAAAACGTTGTGG
TTATCGGCGGTGGATCTGTAGGTGTAGAAACGGCTGAGTATATGGCCTTTTGCGGCAGTTCTATTACCCTGGTGGAAATG
AGAAATGAAATTCTACCGGGGCTGGAGCGAGAAACGAAAATCGGCCTCAAAGAGGCGATTCGTCATTACCGTATGAACGT
TATAAAGTCAGCAAAAGTGGTGGGTATTGGTGAGGATCATGTGATTATCGAGAAAAACGGCAAACAGATCACTCTCGATG
AGGTCGATACCCTGGTGGTCGCGGCAGGCTCCCGCTCTATCAACCAGCTGGAAACTCCACTGCGCGAGGCGGGGCTGTTA
ACCCAGGTGATTGGTGATGCCAAACAGGTGCGCAACGGCCTGGCTGCCATGCACGAAGGCTATCTTGCCGGCTATGGAGT
ATGA

Upstream 100 bases:

>100_bases
GATTGTGACGTCTATTTCTACCTTGACGGCGACTGATGAAGGTTATGCGACTGAGCAGCTGATGGCATATTTGGAACGCA
AGGCCCGGGGTGGATGGGCA

Downstream 100 bases:

>100_bases
AAAACGCTCTGAGGATCGTTTCAATATTGCTCACTCTTTAACGTCAGGCTTTATTCTCGGCCGCGTGCCGGCTCCCCTCA
ACGGCCATGGGAGCCGGCTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MIITEYYAIAPHVGFFPRALGIWNDDLIKNHSELTRRVHGARAKIAAQISHAGREIYRDHPRENAVSSSALKDVYGEFRP
REITLPEIKKIVSQYGDTAKNLKKANFDAVEIYAAHGFLISNFLSKYTNHRTDEYGGSLRDRMRFLEEIVKDVRSKVGAD
FPVLVRLSTKEFVPGGLSLAETRVIVKRMEELGIDAINCSQGIVTVSNNIVEPFQVGNAPFVDIAEEIKKIVSIPVITTG
RINDADLAETTLLAGKSDFIGMARASLADPDLPKKVLAGRWDEIRHCIGCNQGCIGNNMRGEVCRCMVNPEINREYLLDL
NPAVEKKNVTIIGGGIAGCEAAIAAARKGHNVTLHEKSDRLGGTWLIASLPLSKGELVSLVKWQISEMKRLNIHLCVNSS
PTVEEIVASCPDKVIIATGSQPILPPIKGIESKIVVQANKILTQDAPFGENVVVIGGGSVGVETAEYMAFCGSSITLVEM
RNEILPGLERETKIGLKEAIRHYRMNVIKSAKVVGIGEDHVIIEKNGKQITLDEVDTLVVAAGSRSINQLETPLREAGLL
TQVIGDAKQVRNGLAAMHEGYLAGYGV

Sequences:

>Translated_587_residues
MIITEYYAIAPHVGFFPRALGIWNDDLIKNHSELTRRVHGARAKIAAQISHAGREIYRDHPRENAVSSSALKDVYGEFRP
REITLPEIKKIVSQYGDTAKNLKKANFDAVEIYAAHGFLISNFLSKYTNHRTDEYGGSLRDRMRFLEEIVKDVRSKVGAD
FPVLVRLSTKEFVPGGLSLAETRVIVKRMEELGIDAINCSQGIVTVSNNIVEPFQVGNAPFVDIAEEIKKIVSIPVITTG
RINDADLAETTLLAGKSDFIGMARASLADPDLPKKVLAGRWDEIRHCIGCNQGCIGNNMRGEVCRCMVNPEINREYLLDL
NPAVEKKNVTIIGGGIAGCEAAIAAARKGHNVTLHEKSDRLGGTWLIASLPLSKGELVSLVKWQISEMKRLNIHLCVNSS
PTVEEIVASCPDKVIIATGSQPILPPIKGIESKIVVQANKILTQDAPFGENVVVIGGGSVGVETAEYMAFCGSSITLVEM
RNEILPGLERETKIGLKEAIRHYRMNVIKSAKVVGIGEDHVIIEKNGKQITLDEVDTLVVAAGSRSINQLETPLREAGLL
TQVIGDAKQVRNGLAAMHEGYLAGYGV
>Mature_587_residues
MIITEYYAIAPHVGFFPRALGIWNDDLIKNHSELTRRVHGARAKIAAQISHAGREIYRDHPRENAVSSSALKDVYGEFRP
REITLPEIKKIVSQYGDTAKNLKKANFDAVEIYAAHGFLISNFLSKYTNHRTDEYGGSLRDRMRFLEEIVKDVRSKVGAD
FPVLVRLSTKEFVPGGLSLAETRVIVKRMEELGIDAINCSQGIVTVSNNIVEPFQVGNAPFVDIAEEIKKIVSIPVITTG
RINDADLAETTLLAGKSDFIGMARASLADPDLPKKVLAGRWDEIRHCIGCNQGCIGNNMRGEVCRCMVNPEINREYLLDL
NPAVEKKNVTIIGGGIAGCEAAIAAARKGHNVTLHEKSDRLGGTWLIASLPLSKGELVSLVKWQISEMKRLNIHLCVNSS
PTVEEIVASCPDKVIIATGSQPILPPIKGIESKIVVQANKILTQDAPFGENVVVIGGGSVGVETAEYMAFCGSSITLVEM
RNEILPGLERETKIGLKEAIRHYRMNVIKSAKVVGIGEDHVIIEKNGKQITLDEVDTLVVAAGSRSINQLETPLREAGLL
TQVIGDAKQVRNGLAAMHEGYLAGYGV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=450, Percent_Identity=36.6666666666667, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1787939, Length=293, Percent_Identity=29.3515358361775, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=245, Percent_Identity=30.6122448979592, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI72001454, Length=248, Percent_Identity=34.2741935483871, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17564188, Length=245, Percent_Identity=30.6122448979592, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=251, Percent_Identity=31.4741035856574, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=247, Percent_Identity=29.9595141700405, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17566914, Length=270, Percent_Identity=28.1481481481481, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=240, Percent_Identity=29.1666666666667, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=300, Percent_Identity=29.6666666666667, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6325086, Length=302, Percent_Identity=26.8211920529801, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 64066; Mature: 64066

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITEYYAIAPHVGFFPRALGIWNDDLIKNHSELTRRVHGARAKIAAQISHAGREIYRDH
CEEEEEEECCCCCCCCHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRENAVSSSALKDVYGEFRPREITLPEIKKIVSQYGDTAKNLKKANFDAVEIYAAHGFLI
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEHHHHHH
SNFLSKYTNHRTDEYGGSLRDRMRFLEEIVKDVRSKVGADFPVLVRLSTKEFVPGGLSLA
HHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCHHH
ETRVIVKRMEELGIDAINCSQGIVTVSNNIVEPFQVGNAPFVDIAEEIKKIVSIPVITTG
HHHHHHHHHHHHCCCEEECCCCEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEC
RINDADLAETTLLAGKSDFIGMARASLADPDLPKKVLAGRWDEIRHCIGCNQGCIGNNMR
CCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCCC
GEVCRCMVNPEINREYLLDLNPAVEKKNVTIIGGGIAGCEAAIAAARKGHNVTLHEKSDR
CCCEEEECCCCCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEECCCCC
LGGTWLIASLPLSKGELVSLVKWQISEMKRLNIHLCVNSSPTVEEIVASCPDKVIIATGS
CCCEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHCCCCEEEEECC
QPILPPIKGIESKIVVQANKILTQDAPFGENVVVIGGGSVGVETAEYMAFCGSSITLVEM
CCCCCCCCCCCCEEEEEEHHHEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEH
RNEILPGLERETKIGLKEAIRHYRMNVIKSAKVVGIGEDHVIIEKNGKQITLDEVDTLVV
HHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCEEECCCCCEEEE
AAGSRSINQLETPLREAGLLTQVIGDAKQVRNGLAAMHEGYLAGYGV
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIITEYYAIAPHVGFFPRALGIWNDDLIKNHSELTRRVHGARAKIAAQISHAGREIYRDH
CEEEEEEECCCCCCCCHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRENAVSSSALKDVYGEFRPREITLPEIKKIVSQYGDTAKNLKKANFDAVEIYAAHGFLI
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEHHHHHH
SNFLSKYTNHRTDEYGGSLRDRMRFLEEIVKDVRSKVGADFPVLVRLSTKEFVPGGLSLA
HHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCHHH
ETRVIVKRMEELGIDAINCSQGIVTVSNNIVEPFQVGNAPFVDIAEEIKKIVSIPVITTG
HHHHHHHHHHHHCCCEEECCCCEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEC
RINDADLAETTLLAGKSDFIGMARASLADPDLPKKVLAGRWDEIRHCIGCNQGCIGNNMR
CCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCCC
GEVCRCMVNPEINREYLLDLNPAVEKKNVTIIGGGIAGCEAAIAAARKGHNVTLHEKSDR
CCCEEEECCCCCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEECCCCC
LGGTWLIASLPLSKGELVSLVKWQISEMKRLNIHLCVNSSPTVEEIVASCPDKVIIATGS
CCCEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHCCCCEEEEECC
QPILPPIKGIESKIVVQANKILTQDAPFGENVVVIGGGSVGVETAEYMAFCGSSITLVEM
CCCCCCCCCCCCEEEEEEHHHEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEH
RNEILPGLERETKIGLKEAIRHYRMNVIKSAKVVGIGEDHVIIEKNGKQITLDEVDTLVV
HHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCEEECCCCCEEEE
AAGSRSINQLETPLREAGLLTQVIGDAKQVRNGLAAMHEGYLAGYGV
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]