Click here to switch to the map view.

The map label for this gene is iolE

Identifier: 157372888

GI number: 157372888

Start: 5143643

End: 5144539

Strand: Reverse

Name: iolE

Synonym: Spro_4656

Alternate gene names: 157372888

Gene position: 5144539-5143643 (Counterclockwise)

Preceding gene: 157372889

Following gene: 157372885

Centisome position: 94.42

GC content: 53.96

Gene sequence:

>897_bases
ATGAATAAAGACAACGTAAAACTGGCCATCGCCCCGATTGGCTGGACCAATGACGACATGCCTGACCTGGGCAAAGAGAA
TACTTTCCAACAGTGCGTCAGTGAAATGGCGCTGGCCGGGTTCACCGGTAGCGAAGTGGGCAGCAAGTACCCGCGCGATC
CGGCGGTACTGAAACCGATGCTGGATATCCGCGGCATTCAGATCTGCAACGCCTGGTTCAGCACCTTCTTCGCCGATGGC
CTGAAAGAAAAAACCATCGATGAGTTTATTAATCATATGAATTTCCTGCATGCGATGGGGGCCAAAGTGATTGGCTGTTC
CGAGCAGAGCAAGAGTATTCAGGGCACCACCAAAGGGGTGTTCGAAGAGAAGCCATATTTCAGCGATGAAGAGTGGCAGC
GGGTGGCCGATGGCTACAATGAGCTGGCGGATATTGCCAAAGGCAAAGGGATGCAGGTCTGCCTGCACCACCATATGGGC
ACCGGCATCCAGACTACCGCCGAGATTGACCGCTACATGAGCATGGTGAACGACGACGTCTACCTGCTGTTTGATACCGG
CCACGCCTACTACTCCGAAGGCAGCCAGCAGGCGATGATGGCAATTCTGGAAAAATATCTGCCGCGCATTAACCATGTGC
ATTTGAAAGACGTGCGCGACGAAGTGGTGGCAGAGGTGAAGGCCAACAAACTGAGCTTCCTCGACGGTGTGAAAAAAGGC
ACCTTCACCGTGCCGGGTGACGGTGTTATCGACTTCCGGCCGGTATTTAAACTACTGGACGAGCGCGGTTATAAAGGCTG
GATGGTGGTGGAGGCCGAGCAGGATCCGGCGTTGGCCAATCCGTTTGAGTATGCGGTGAAGGCACGGCGTTATATCAAAG
AGACTGCCGGCATTTAA

Upstream 100 bases:

>100_bases
CGACCCGCGAGCGAATCGAAAAAGAGCTGCTGGGTAAATAAATTTCTCGGCTGCGCGGGTTGGCATCACCGTCGGGCCGT
TTCGACTATTGAGGCTAAAA

Downstream 100 bases:

>100_bases
TCCGGCCGTGATCTGCAAGGGGCGCTTGATTAATCAATGCGCCCCTTTTTATTCTGTCTGTCAGACGAGGGTTTTCCAGG
TGGTGACCTGTCCGCCGGGC

Product: xylose isomerase domain-containing protein

Products: NA

Alternate protein names: 2-keto-myo-inositol dehydratase; 2KMI dehydratase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNKDNVKLAIAPIGWTNDDMPDLGKENTFQQCVSEMALAGFTGSEVGSKYPRDPAVLKPMLDIRGIQICNAWFSTFFADG
LKEKTIDEFINHMNFLHAMGAKVIGCSEQSKSIQGTTKGVFEEKPYFSDEEWQRVADGYNELADIAKGKGMQVCLHHHMG
TGIQTTAEIDRYMSMVNDDVYLLFDTGHAYYSEGSQQAMMAILEKYLPRINHVHLKDVRDEVVAEVKANKLSFLDGVKKG
TFTVPGDGVIDFRPVFKLLDERGYKGWMVVEAEQDPALANPFEYAVKARRYIKETAGI

Sequences:

>Translated_298_residues
MNKDNVKLAIAPIGWTNDDMPDLGKENTFQQCVSEMALAGFTGSEVGSKYPRDPAVLKPMLDIRGIQICNAWFSTFFADG
LKEKTIDEFINHMNFLHAMGAKVIGCSEQSKSIQGTTKGVFEEKPYFSDEEWQRVADGYNELADIAKGKGMQVCLHHHMG
TGIQTTAEIDRYMSMVNDDVYLLFDTGHAYYSEGSQQAMMAILEKYLPRINHVHLKDVRDEVVAEVKANKLSFLDGVKKG
TFTVPGDGVIDFRPVFKLLDERGYKGWMVVEAEQDPALANPFEYAVKARRYIKETAGI
>Mature_298_residues
MNKDNVKLAIAPIGWTNDDMPDLGKENTFQQCVSEMALAGFTGSEVGSKYPRDPAVLKPMLDIRGIQICNAWFSTFFADG
LKEKTIDEFINHMNFLHAMGAKVIGCSEQSKSIQGTTKGVFEEKPYFSDEEWQRVADGYNELADIAKGKGMQVCLHHHMG
TGIQTTAEIDRYMSMVNDDVYLLFDTGHAYYSEGSQQAMMAILEKYLPRINHVHLKDVRDEVVAEVKANKLSFLDGVKKG
TFTVPGDGVIDFRPVFKLLDERGYKGWMVVEAEQDPALANPFEYAVKARRYIKETAGI

Specific function: Catalyzes the dehydration of inosose (2-keto-myo- inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi- inositol)

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iolE/mocC family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLE_SERP5 (A8GKV9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480877.1
- ProteinModelPortal:   A8GKV9
- SMR:   A8GKV9
- STRING:   A8GKV9
- GeneID:   5603058
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4656
- eggNOG:   COG1082
- HOGENOM:   HBG654552
- OMA:   DLGKENT
- ProtClustDB:   CLSK957433
- BioCyc:   SPRO399741:SPRO_4656-MONOMER
- HAMAP:   MF_01672
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =4.2.1.44

Molecular weight: Translated: 33428; Mature: 33428

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDNVKLAIAPIGWTNDDMPDLGKENTFQQCVSEMALAGFTGSEVGSKYPRDPAVLKPM
CCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHH
LDIRGIQICNAWFSTFFADGLKEKTIDEFINHMNFLHAMGAKVIGCSEQSKSIQGTTKGV
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCH
FEEKPYFSDEEWQRVADGYNELADIAKGKGMQVCLHHHMGTGIQTTAEIDRYMSMVNDDV
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCE
YLLFDTGHAYYSEGSQQAMMAILEKYLPRINHVHLKDVRDEVVAEVKANKLSFLDGVKKG
EEEEECCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TFTVPGDGVIDFRPVFKLLDERGYKGWMVVEAEQDPALANPFEYAVKARRYIKETAGI
CEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKDNVKLAIAPIGWTNDDMPDLGKENTFQQCVSEMALAGFTGSEVGSKYPRDPAVLKPM
CCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHH
LDIRGIQICNAWFSTFFADGLKEKTIDEFINHMNFLHAMGAKVIGCSEQSKSIQGTTKGV
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCH
FEEKPYFSDEEWQRVADGYNELADIAKGKGMQVCLHHHMGTGIQTTAEIDRYMSMVNDDV
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCE
YLLFDTGHAYYSEGSQQAMMAILEKYLPRINHVHLKDVRDEVVAEVKANKLSFLDGVKKG
EEEEECCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TFTVPGDGVIDFRPVFKLLDERGYKGWMVVEAEQDPALANPFEYAVKARRYIKETAGI
CEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA