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The map label for this gene is bioH

Identifier: 157372864

GI number: 157372864

Start: 5109611

End: 5110405

Strand: Reverse

Name: bioH

Synonym: Spro_4632

Alternate gene names: 157372864

Gene position: 5110405-5109611 (Counterclockwise)

Preceding gene: 157372867

Following gene: 157372863

Centisome position: 93.79

GC content: 57.36

Gene sequence:

>795_bases
ATGACAGCGTTGTACTGGCAAACAATAGGTGAAGGCGATCGCGATCTTGTGCTGCTGCACGGCTGGGGGCTGAACGCGGA
AGTATGGAGTTGCATGCTTGAGCGGCTGACGCCGCATTTTCGTCTGCATCTGGTCGATCTGCCGGGTTATGGCCGCAGCC
AGGGTTTTGCTGCCATGTCGCTTGAGCAAATGGCGGAAATTGTGTTGGACGCCGCGCCGGCGCAGGCCTGGTGGTTGGGT
TGGTCGCTCGGCGGGTTGGTGGCCAGCCAGATTGCGTTGACGCAGCCACAACGGGTGCATGGGTTGATTACCGTGGCCTC
TTCACCCTGTTTTGCGGCGCAGGATGATTGGCCGGGGATCCGCCCGGAGGTGCTGAGTGGTTTTCAGCATCAGCTGAGCC
AGGATTTCCAGCGCACCGTAGAGCGTTTTCTGGCATTACAAACGTTGGGCACGGAAAGTGCGCGGCAGGACGCCCGCCTG
CTGAAATCGGTGGTGCTTAACCAGCCGATGCCGAGCGTTGAAATACTGAACGGCGGGCTTGAGATACTGCGGACCGCTGA
CCTCCGTCAGTCTTTGGCGGGGCTGTCACTGCCACTGTTGCGGATTTACGGTTATCTGGACGGATTAGTGCCGCGTAAGG
TCGCCGGATTGCTCGACGTCAGTTGGCCTCACTCATCATCAATCATCATTGCGAAAGCGGCTCATGCGCCATTCATTTCC
CAGCCTGATGAATTTGCAGAAATTATTGGCACTTTTGTGGCCGAAAATGGGATCTGCCGCCAAAACGCACAATAA

Upstream 100 bases:

>100_bases
GATGGCTGAGGCGTAATGGCTGTCGGCATAGCCAACAGCGGCTGTAGATTGATAGCATAGCCGTCCTCCCTGACGGATGA
GAATGAAAGGACAATAACCG

Downstream 100 bases:

>100_bases
AGCGCTAAAAAAAGAGCGGTTTAGTGGATAAAAAATAAAGCTGTCCGATACTTAAGGGGGTAACTGCGTTGGTTGAAAAT
GAAAAAATCACCCATGGTTT

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTALYWQTIGEGDRDLVLLHGWGLNAEVWSCMLERLTPHFRLHLVDLPGYGRSQGFAAMSLEQMAEIVLDAAPAQAWWLG
WSLGGLVASQIALTQPQRVHGLITVASSPCFAAQDDWPGIRPEVLSGFQHQLSQDFQRTVERFLALQTLGTESARQDARL
LKSVVLNQPMPSVEILNGGLEILRTADLRQSLAGLSLPLLRIYGYLDGLVPRKVAGLLDVSWPHSSSIIIAKAAHAPFIS
QPDEFAEIIGTFVAENGICRQNAQ

Sequences:

>Translated_264_residues
MTALYWQTIGEGDRDLVLLHGWGLNAEVWSCMLERLTPHFRLHLVDLPGYGRSQGFAAMSLEQMAEIVLDAAPAQAWWLG
WSLGGLVASQIALTQPQRVHGLITVASSPCFAAQDDWPGIRPEVLSGFQHQLSQDFQRTVERFLALQTLGTESARQDARL
LKSVVLNQPMPSVEILNGGLEILRTADLRQSLAGLSLPLLRIYGYLDGLVPRKVAGLLDVSWPHSSSIIIAKAAHAPFIS
QPDEFAEIIGTFVAENGICRQNAQ
>Mature_263_residues
TALYWQTIGEGDRDLVLLHGWGLNAEVWSCMLERLTPHFRLHLVDLPGYGRSQGFAAMSLEQMAEIVLDAAPAQAWWLGW
SLGGLVASQIALTQPQRVHGLITVASSPCFAAQDDWPGIRPEVLSGFQHQLSQDFQRTVERFLALQTLGTESARQDARLL
KSVVLNQPMPSVEILNGGLEILRTADLRQSLAGLSLPLLRIYGYLDGLVPRKVAGLLDVSWPHSSSIIIAKAAHAPFISQ
PDEFAEIIGTFVAENGICRQNAQ

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=249, Percent_Identity=70.281124497992, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_SERP5 (A8GKT5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480853.1
- ProteinModelPortal:   A8GKT5
- STRING:   A8GKT5
- GeneID:   5604262
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4632
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   QGPDLVM
- BioCyc:   SPRO399741:SPRO_4632-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 28986; Mature: 28854

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: ACT_SITE 82-82 ACT_SITE 207-207 ACT_SITE 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALYWQTIGEGDRDLVLLHGWGLNAEVWSCMLERLTPHFRLHLVDLPGYGRSQGFAAMS
CCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHH
LEQMAEIVLDAAPAQAWWLGWSLGGLVASQIALTQPQRVHGLITVASSPCFAAQDDWPGI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCC
RPEVLSGFQHQLSQDFQRTVERFLALQTLGTESARQDARLLKSVVLNQPMPSVEILNGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
EILRTADLRQSLAGLSLPLLRIYGYLDGLVPRKVAGLLDVSWPHSSSIIIAKAAHAPFIS
HHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
QPDEFAEIIGTFVAENGICRQNAQ
CCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TALYWQTIGEGDRDLVLLHGWGLNAEVWSCMLERLTPHFRLHLVDLPGYGRSQGFAAMS
CEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHH
LEQMAEIVLDAAPAQAWWLGWSLGGLVASQIALTQPQRVHGLITVASSPCFAAQDDWPGI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCC
RPEVLSGFQHQLSQDFQRTVERFLALQTLGTESARQDARLLKSVVLNQPMPSVEILNGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
EILRTADLRQSLAGLSLPLLRIYGYLDGLVPRKVAGLLDVSWPHSSSIIIAKAAHAPFIS
HHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
QPDEFAEIIGTFVAENGICRQNAQ
CCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA