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The map label for this gene is pmbA [H]

Identifier: 157372617

GI number: 157372617

Start: 4849980

End: 4851320

Strand: Reverse

Name: pmbA [H]

Synonym: Spro_4384

Alternate gene names: 157372617

Gene position: 4851320-4849980 (Counterclockwise)

Preceding gene: 157372618

Following gene: 157372616

Centisome position: 89.03

GC content: 58.61

Gene sequence:

>1341_bases
ATGAAAGTAGTCACTCAAGTTGCACAGCAGCGCAAGGCGCTGGAACAGGCCGTTTCACAGGCTTTGGAACTGGCGCGCGC
CGGTTCCGATGCGGCAGAAGTTGCCGTGACCAAATCTACCGGTATCAGCGTCAGCACCCGCTTTGGCGAGGTGGAAAACG
TTGAATTCAACAGCGATGGTGCGTTGGGTATTACCGTCTATCACCAGCAACGTAAAGGCAGCGCTTCATCAACCGATCTC
AGCCCGGACGCTATTGCCCGCACCGTGCAGGCGGCGCTGGACATTGCCCGTTATACCTCAGTGGATCCCCATGCCGGCCC
GGCGGAGAAAGACCTGCTGGCGTTTGACGCGCCGGACCTGGATCTGTTCCACCCGGCAGAGCTGGACGCCGATCGCGGTA
TCGAGCTGGCTGCCCGCGCCGAACAGGCATCCCTGGCGGCGGACAAGCGCATCACCAATACCGAAGGCGGCAGTTTTAAC
AGCCACTATGGCATCAAGGTGTTCGGCAACAGTCACGGCATGCTGCAAAGCTATTGTTCCAGCCGACATTCATTGTCGAG
CTGTGTGATCGCCGAGCAAGGTGGTGATATGGAGCGTGATTACGCCTATACCATCGGCCGTGCGATGAGCGATTTGCAAA
GTCCTGAATGGGTGGGACAGGAATGTGCCCGCCGCACCTTGTCACGCCTGGCACCGCGTAAGCTATCGACCATGACCGCT
CCGGTGCTGTTTGCCTCTGAAGTGGCGACCGGTCTGTTCGGCCATCTGGTTGGTGCCATCAGCGGCAGCAGCGTTTACCG
TAAATCGACCTTCCTGTTGGATTCGCTGGGCAAACAAATCTTGCCGGAGTGGCTGACCATCGAAGAGCACCCGCATCTGT
TGAAGGGGCTGGCTTCCACGCCGTTCGACAGCGAAGGCGTGCGTACCCAACGGCGCGACATTGTCAAAGACGGCGTACTG
CAAACCTGGCTGATGACCAGCTATGCGGCGCGTAAGCTCGGCATGCACAGCACCGGCCATGCGGGCGGCATCCATAACTG
GCGTATTGCCGGTCAGGGTAATGACTTTGCCGGCATGTTGAAACAGTTGGGCACCGGCCTGGTGGTCACCGAACTGATGG
GGCAGGGCGTCAGCGGTATTACCGGTGACTACTCACGCGGCGCGGCTGGTTTCTGGGTGGAAAATGGCGAGATCCAATAT
CCGGTCAGCGAGATCACCATCGCCGGCAACCTGAAAGATATGCTGCGCAATATCGTCAGCGTGGGCAGCGATATCGAAAC
CCGCAGCAACATCCAGTGTGGTTCCGTGTTGTTGCCGGAAATGAAAATCGCCGGTCAGTAA

Upstream 100 bases:

>100_bases
TCAAAAGTATAAATCTCATGATTTTTGCGCGTGACTCCACGCGCAACCACGCCGCCACGGCACTTTTCTCAACGATTTTC
TGATTAAGTATGGCAGATTG

Downstream 100 bases:

>100_bases
ATCTTCTTATGGCTGGCCGCTCCGGTTGCCAGCCGCATGACTCCCCCTTCCCCGTTTTAACCTTGCTTACAAATCTTCGG
TTTCGGTTTTCAGCCCTCTT

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKVVTQVAQQRKALEQAVSQALELARAGSDAAEVAVTKSTGISVSTRFGEVENVEFNSDGALGITVYHQQRKGSASSTDL
SPDAIARTVQAALDIARYTSVDPHAGPAEKDLLAFDAPDLDLFHPAELDADRGIELAARAEQASLAADKRITNTEGGSFN
SHYGIKVFGNSHGMLQSYCSSRHSLSSCVIAEQGGDMERDYAYTIGRAMSDLQSPEWVGQECARRTLSRLAPRKLSTMTA
PVLFASEVATGLFGHLVGAISGSSVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASTPFDSEGVRTQRRDIVKDGVL
QTWLMTSYAARKLGMHSTGHAGGIHNWRIAGQGNDFAGMLKQLGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKDMLRNIVSVGSDIETRSNIQCGSVLLPEMKIAGQ

Sequences:

>Translated_446_residues
MKVVTQVAQQRKALEQAVSQALELARAGSDAAEVAVTKSTGISVSTRFGEVENVEFNSDGALGITVYHQQRKGSASSTDL
SPDAIARTVQAALDIARYTSVDPHAGPAEKDLLAFDAPDLDLFHPAELDADRGIELAARAEQASLAADKRITNTEGGSFN
SHYGIKVFGNSHGMLQSYCSSRHSLSSCVIAEQGGDMERDYAYTIGRAMSDLQSPEWVGQECARRTLSRLAPRKLSTMTA
PVLFASEVATGLFGHLVGAISGSSVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASTPFDSEGVRTQRRDIVKDGVL
QTWLMTSYAARKLGMHSTGHAGGIHNWRIAGQGNDFAGMLKQLGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKDMLRNIVSVGSDIETRSNIQCGSVLLPEMKIAGQ
>Mature_446_residues
MKVVTQVAQQRKALEQAVSQALELARAGSDAAEVAVTKSTGISVSTRFGEVENVEFNSDGALGITVYHQQRKGSASSTDL
SPDAIARTVQAALDIARYTSVDPHAGPAEKDLLAFDAPDLDLFHPAELDADRGIELAARAEQASLAADKRITNTEGGSFN
SHYGIKVFGNSHGMLQSYCSSRHSLSSCVIAEQGGDMERDYAYTIGRAMSDLQSPEWVGQECARRTLSRLAPRKLSTMTA
PVLFASEVATGLFGHLVGAISGSSVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASTPFDSEGVRTQRRDIVKDGVL
QTWLMTSYAARKLGMHSTGHAGGIHNWRIAGQGNDFAGMLKQLGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKDMLRNIVSVGSDIETRSNIQCGSVLLPEMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=86.0986547085202, Blast_Score=804, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47771; Mature: 47771

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVTQVAQQRKALEQAVSQALELARAGSDAAEVAVTKSTGISVSTRFGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCEEEECCCCCCCEEECCCC
ALGITVYHQQRKGSASSTDLSPDAIARTVQAALDIARYTSVDPHAGPAEKDLLAFDAPDL
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCC
DLFHPAELDADRGIELAARAEQASLAADKRITNTEGGSFNSHYGIKVFGNSHGMLQSYCS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
SRHSLSSCVIAEQGGDMERDYAYTIGRAMSDLQSPEWVGQECARRTLSRLAPRKLSTMTA
HHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
PVLFASEVATGLFGHLVGAISGSSVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLAST
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHCC
PFDSEGVRTQRRDIVKDGVLQTWLMTSYAARKLGMHSTGHAGGIHNWRIAGQGNDFAGML
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHH
KQLGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMLRNIVS
HHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHHH
VGSDIETRSNIQCGSVLLPEMKIAGQ
CCCCCCCCCCCCCCCEECCCHHCCCC
>Mature Secondary Structure
MKVVTQVAQQRKALEQAVSQALELARAGSDAAEVAVTKSTGISVSTRFGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCEEEECCCCCCCEEECCCC
ALGITVYHQQRKGSASSTDLSPDAIARTVQAALDIARYTSVDPHAGPAEKDLLAFDAPDL
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCC
DLFHPAELDADRGIELAARAEQASLAADKRITNTEGGSFNSHYGIKVFGNSHGMLQSYCS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
SRHSLSSCVIAEQGGDMERDYAYTIGRAMSDLQSPEWVGQECARRTLSRLAPRKLSTMTA
HHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
PVLFASEVATGLFGHLVGAISGSSVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLAST
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHCC
PFDSEGVRTQRRDIVKDGVLQTWLMTSYAARKLGMHSTGHAGGIHNWRIAGQGNDFAGML
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHH
KQLGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMLRNIVS
HHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHHHHHH
VGSDIETRSNIQCGSVLLPEMKIAGQ
CCCCCCCCCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]