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The map label for this gene is rpoN [H]

Identifier: 157372600

GI number: 157372600

Start: 4836042

End: 4837475

Strand: Direct

Name: rpoN [H]

Synonym: Spro_4367

Alternate gene names: 157372600

Gene position: 4836042-4837475 (Clockwise)

Preceding gene: 157372599

Following gene: 157372601

Centisome position: 88.75

GC content: 55.86

Gene sequence:

>1434_bases
ATGAAGCAAGGTTTGCAACTCAGGTTTAGCCAACAACTGGCCATGACCCCCCAGCTCCAGCAGGCTATCCGCCTGCTGCA
GTTGTCCACGCTTGAGCTCCAGCAGGAGATACAGCTGGCGTTAGAGAGCAACCCATTGCTTGAACAGAGCGACCTGCACG
AAGAAGTCGACGCCAAAGAAACTGTCGAAACCGAAGGTTTGGATACCCGCGAGGCGTTGGAACAAAAGGACATGCCCGAA
GAGCTGCCGCTCGACGCCACCTGGGACGAAATCTATACCGCCGGTACGCCGTCCGGTACCCGCACCGATTACAGTGACGA
TGAGCTACCGGTTTATCAGGGCGAGACCACCCAGACCCTGCAGGATTACCTGATGTGGCAAGTGGATCTGACGCCGTTTT
CTGATACCGATGCCGCTATTGCCACCTCAATCGTCGACGCCGTTGACGACACCGGCTATCTCACCGTGCCGCTGGAAGAC
ATTCTGGAGAGTCTGGGTGACGATAATGTGACAATGGACGAAGTCGAAGCGGTATTGAAGCGCGTACAGCGTTTTGATCC
GGTTGGCGTCGCCGCGCGCGACCTGCGCGATTGTCTGCTGGTTCAGCTTTCCCAGTACGCTAAAGACACGCCCTATCTGG
CTGAGGCACGCCTGATCATCAGCGAGCATCTGGACCTGCTGGCTAACCACGACTTTCGCAGCCTGATGCGAACAACGCGC
TCAAAAGAAGATACACTGAAAGAAGCGATGTTGCTGATCCAGTCATTGGATCCCCGTCCGGGTCAGTCGATCAACACCGG
CGAATCCGAGTATGTGATCCCGGACGTGCTGGTGCGCAAAGTGCAGGATGTCTGGACGGTCGAGCTCAATAGCGACAGCA
TTCCACGCTTGAAGATCAACCAGCAGTACGCCGCGATGGGCAATAGCGCACGTAACGACAGTGACGGGCAGTACATCCGC
AGCAATTTGCAGGAAGCGAAATGGCTGATCAAAAGTCTGGAAAGCCGAAACGACACTCTGCTGAAAGTTTCGCGCTGCAT
CGTCGAGCAGCAACAGGCGTTCTTTGAACAGGGCACAGAATTTATGAAGCCCATGGTGCTGGCAGATATTGCCCAGGCCG
TGGAGATGCATGAATCGACAATTTCACGCGTGACCACGCAGAAGTTTTTGCACAGTCCGCGCGGCATTTTCGAATTGAAA
TATTTCTTCTCCAGCCATGTGAATACCGACAGCGGGGGCGAAGCCTCCTCTACGGCGATCCGGGCAGTGGTCAAGAAAAT
GATCGCGGCGGAAAACCCCGCCAAACCGCTCAGCGACAGCAAGCTGGCCACCCTGCTCTCCGACCAGGGGATCATCGTGG
CACGGCGTACCGTCGCCAAGTACCGAGAGTCTTTGTCCATCCCGCCGTCCAACCAACGTAAACAGTTGGTTTGA

Upstream 100 bases:

>100_bases
CCGATGAGCAGGTGAAACGCGTTTATCTGGGCGAAGAATTCCGCCTCTGAGTACCAGCGCGTCCTGATGGACGACTGTTA
ACGGAAGTAGACTGAAGATT

Downstream 100 bases:

>100_bases
CCTCTATTGAGAAGGAAGACATTATGCAGCTCAACATTACCGGACACCACATCGAAATCACCGAACCTTTGCGCGAGTTC
GTGAACACCAAGTTCGCCAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKQGLQLRFSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQSDLHEEVDAKETVETEGLDTREALEQKDMPE
ELPLDATWDEIYTAGTPSGTRTDYSDDELPVYQGETTQTLQDYLMWQVDLTPFSDTDAAIATSIVDAVDDTGYLTVPLED
ILESLGDDNVTMDEVEAVLKRVQRFDPVGVAARDLRDCLLVQLSQYAKDTPYLAEARLIISEHLDLLANHDFRSLMRTTR
SKEDTLKEAMLLIQSLDPRPGQSINTGESEYVIPDVLVRKVQDVWTVELNSDSIPRLKINQQYAAMGNSARNDSDGQYIR
SNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEQGTEFMKPMVLADIAQAVEMHESTISRVTTQKFLHSPRGIFELK
YFFSSHVNTDSGGEASSTAIRAVVKKMIAAENPAKPLSDSKLATLLSDQGIIVARRTVAKYRESLSIPPSNQRKQLV

Sequences:

>Translated_477_residues
MKQGLQLRFSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQSDLHEEVDAKETVETEGLDTREALEQKDMPE
ELPLDATWDEIYTAGTPSGTRTDYSDDELPVYQGETTQTLQDYLMWQVDLTPFSDTDAAIATSIVDAVDDTGYLTVPLED
ILESLGDDNVTMDEVEAVLKRVQRFDPVGVAARDLRDCLLVQLSQYAKDTPYLAEARLIISEHLDLLANHDFRSLMRTTR
SKEDTLKEAMLLIQSLDPRPGQSINTGESEYVIPDVLVRKVQDVWTVELNSDSIPRLKINQQYAAMGNSARNDSDGQYIR
SNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEQGTEFMKPMVLADIAQAVEMHESTISRVTTQKFLHSPRGIFELK
YFFSSHVNTDSGGEASSTAIRAVVKKMIAAENPAKPLSDSKLATLLSDQGIIVARRTVAKYRESLSIPPSNQRKQLV
>Mature_477_residues
MKQGLQLRFSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQSDLHEEVDAKETVETEGLDTREALEQKDMPE
ELPLDATWDEIYTAGTPSGTRTDYSDDELPVYQGETTQTLQDYLMWQVDLTPFSDTDAAIATSIVDAVDDTGYLTVPLED
ILESLGDDNVTMDEVEAVLKRVQRFDPVGVAARDLRDCLLVQLSQYAKDTPYLAEARLIISEHLDLLANHDFRSLMRTTR
SKEDTLKEAMLLIQSLDPRPGQSINTGESEYVIPDVLVRKVQDVWTVELNSDSIPRLKINQQYAAMGNSARNDSDGQYIR
SNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEQGTEFMKPMVLADIAQAVEMHESTISRVTTQKFLHSPRGIFELK
YFFSSHVNTDSGGEASSTAIRAVVKKMIAAENPAKPLSDSKLATLLSDQGIIVARRTVAKYRESLSIPPSNQRKQLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=85.1153039832285, Blast_Score=838, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53642; Mature: 53642

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLQLRFSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQSDLHEEVDAKE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHH
TVETEGLDTREALEQKDMPEELPLDATWDEIYTAGTPSGTRTDYSDDELPVYQGETTQTL
HHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVDLTPFSDTDAAIATSIVDAVDDTGYLTVPLEDILESLGDDNVTMDEVEAVLK
HHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCHHHHHHHHH
RVQRFDPVGVAARDLRDCLLVQLSQYAKDTPYLAEARLIISEHLDLLANHDFRSLMRTTR
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHC
SKEDTLKEAMLLIQSLDPRPGQSINTGESEYVIPDVLVRKVQDVWTVELNSDSIPRLKIN
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHEEEEECCCCCCCEEEC
QQYAAMGNSARNDSDGQYIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEQGTE
CHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FMKPMVLADIAQAVEMHESTISRVTTQKFLHSPRGIFELKYFFSSHVNTDSGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RAVVKKMIAAENPAKPLSDSKLATLLSDQGIIVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQGLQLRFSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQSDLHEEVDAKE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHH
TVETEGLDTREALEQKDMPEELPLDATWDEIYTAGTPSGTRTDYSDDELPVYQGETTQTL
HHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVDLTPFSDTDAAIATSIVDAVDDTGYLTVPLEDILESLGDDNVTMDEVEAVLK
HHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCHHHHHHHHH
RVQRFDPVGVAARDLRDCLLVQLSQYAKDTPYLAEARLIISEHLDLLANHDFRSLMRTTR
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHC
SKEDTLKEAMLLIQSLDPRPGQSINTGESEYVIPDVLVRKVQDVWTVELNSDSIPRLKIN
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHEEEEECCCCCCCEEEC
QQYAAMGNSARNDSDGQYIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEQGTE
CHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FMKPMVLADIAQAVEMHESTISRVTTQKFLHSPRGIFELKYFFSSHVNTDSGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RAVVKKMIAAENPAKPLSDSKLATLLSDQGIIVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]