Click here to switch to the map view.

The map label for this gene is mlaE [H]

Identifier: 157372592

GI number: 157372592

Start: 4829714

End: 4830496

Strand: Reverse

Name: mlaE [H]

Synonym: Spro_4359

Alternate gene names: 157372592

Gene position: 4830496-4829714 (Counterclockwise)

Preceding gene: 157372593

Following gene: 157372591

Centisome position: 88.65

GC content: 56.96

Gene sequence:

>783_bases
ATGTTATTACAGGCGTTAGCGTCGTTAGGACGCCGTGGCATCAACACCAGCGCCAGTTTTGGACGCGCCGGATTGATGCT
GTGGGGCGCGCTGGTCGGGCGGCCCGAGCCGCGCAAACAGTGGCCGTTGCTGCTCAAGCAAATGCACAGTGTAGGCGTGC
AATCACTGCTGATTATCATGGTTTCCGGGTTGTTTATCGGCATGGTACTGGGGCTACAGGGCTATTTGGTGCTGACCACC
TACAGTGCCGAGGCCAGCTTGGGCATGATGGTGGCGCTGTCGCTGTTACGTGAACTGGGCCCGGTGGTGACGGCACTATT
GTTTGCCGGTCGTGCCGGTTCCGCGCTGACGGCCGAAATTGGCCTGATGAAGGCAACCGAACAAATCTCCAGCCTGGAAA
TGATGGCGGTCGACCCGCTGCGGCGTATTGTCGCACCGCGCTTCTGGGCCGGTTTGATCAGCATGCCGTTGTTGACGCTG
ATCTTTGTCGCAATTGGTATCTGGGGCGGTTCGATAGTCGGTGTGGATTGGAAGGGCATTGACGGTGGCTTCTTCTGGTC
GGCGATGCAGGGAGCCGTAGAGTGGAAAAAAGACTTGCTCAACTGCCTGCTCAAGAGCGTGGTATTTGCCATTACCGTGA
CCTGGATTGCCATCTTCAACGGGTATGATGCAGTACCGACCTCTGAAGGGATTAGCCGGGCAACGACCCGTACCGTGGTA
CATTCGTCACTGGCGGTGTTGGGATTGGATTTCGTGCTGACAGCACTGATGTTTGGGAATTGA

Upstream 100 bases:

>100_bases
CGCCAGTTCCTGGATGGTATTGCCGATGGGCCGGTGCCTTTCCGTTATCCGGCTGGCGATTATCAGGCCGAGTTGTTAGG
TTTAGGGAGTAAATAAGCTG

Downstream 100 bases:

>100_bases
TTCGATGCAAACGAAGAAGAGTGAAATCTGGGTTGGGGCATTTATGCTGATTGCGCTGTGCGCCGTCGTGTTTTTGTGCC
TGCAAGTGGCAAACATTAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLQALASLGRRGINTSASFGRAGLMLWGALVGRPEPRKQWPLLLKQMHSVGVQSLLIIMVSGLFIGMVLGLQGYLVLTT
YSAEASLGMMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAVDPLRRIVAPRFWAGLISMPLLTL
IFVAIGIWGGSIVGVDWKGIDGGFFWSAMQGAVEWKKDLLNCLLKSVVFAITVTWIAIFNGYDAVPTSEGISRATTRTVV
HSSLAVLGLDFVLTALMFGN

Sequences:

>Translated_260_residues
MLLQALASLGRRGINTSASFGRAGLMLWGALVGRPEPRKQWPLLLKQMHSVGVQSLLIIMVSGLFIGMVLGLQGYLVLTT
YSAEASLGMMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAVDPLRRIVAPRFWAGLISMPLLTL
IFVAIGIWGGSIVGVDWKGIDGGFFWSAMQGAVEWKKDLLNCLLKSVVFAITVTWIAIFNGYDAVPTSEGISRATTRTVV
HSSLAVLGLDFVLTALMFGN
>Mature_260_residues
MLLQALASLGRRGINTSASFGRAGLMLWGALVGRPEPRKQWPLLLKQMHSVGVQSLLIIMVSGLFIGMVLGLQGYLVLTT
YSAEASLGMMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAVDPLRRIVAPRFWAGLISMPLLTL
IFVAIGIWGGSIVGVDWKGIDGGFFWSAMQGAVEWKKDLLNCLLKSVVFAITVTWIAIFNGYDAVPTSEGISRATTRTVV
HSSLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=260, Percent_Identity=81.5384615384615, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27828; Mature: 27828

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLQALASLGRRGINTSASFGRAGLMLWGALVGRPEPRKQWPLLLKQMHSVGVQSLLIIM
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VSGLFIGMVLGLQGYLVLTTYSAEASLGMMVALSLLRELGPVVTALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMKATEQISSLEMMAVDPLRRIVAPRFWAGLISMPLLTLIFVAIGIWGGSIVGVDWKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
DGGFFWSAMQGAVEWKKDLLNCLLKSVVFAITVTWIAIFNGYDAVPTSEGISRATTRTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLQALASLGRRGINTSASFGRAGLMLWGALVGRPEPRKQWPLLLKQMHSVGVQSLLIIM
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VSGLFIGMVLGLQGYLVLTTYSAEASLGMMVALSLLRELGPVVTALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMKATEQISSLEMMAVDPLRRIVAPRFWAGLISMPLLTLIFVAIGIWGGSIVGVDWKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
DGGFFWSAMQGAVEWKKDLLNCLLKSVVFAITVTWIAIFNGYDAVPTSEGISRATTRTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]