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The map label for this gene is rlmG

Identifier: 157372545

GI number: 157372545

Start: 4782933

End: 4784072

Strand: Reverse

Name: rlmG

Synonym: Spro_4312

Alternate gene names: 157372545

Gene position: 4784072-4782933 (Counterclockwise)

Preceding gene: 157372553

Following gene: 157372541

Centisome position: 87.8

GC content: 56.93

Gene sequence:

>1140_bases
ATGAGCCAACTCGATCTGGGAACACAGCAACTTGAGCTGGAGCGTTATCCCCAACAGGAAGAATCCACCCAACTACAGGC
GTGGGAAGCGGCGGACGAATATCTGCTGCAACAGCTTGAAAATGTAAACATTGGCGATCGTCCGGTGCTGATTTTCAACG
ACAACTTTGGCACGCTGGCCTGCGCACTGCACAGCCATCAACCTTACAGCATCAGCGATTCCTACATGAGCCAGTTGGCC
ACGCGCCATAACCTGAAGCTCAATGAATTGGATCCGCAGCAGGTTACGCTGCTGGACACCCTGGCCGAATTGCCTGCGTC
ACCGGCGGTGGTGCTGATCCGCATTCCTAAAGCGCTGGCACTGCTGGAACAGCAGCTGCGTGCGTTGCGTGATGTGGTGG
CACCGGACACGGTAATCATCGCAGGGGCCAAAGCGCGCGACGTACATACCTCTACCATGCAGCTGTTTGAAAAGGTGCTC
GGCCCCACTCGCACCAGTCTGGCATGGAAGAAAGCGCGGTTGATCCACTGTGAAGTCGCAGACATCGTGCCACCGGCAGC
GCCGGTAACCACCAACTGGGTGCTGGACGGCACCGATTGGGTGATCCACAACCATGCCAACGTGTTCTCACGCAGCAACC
TGGATATCGGCGCACGCCTGTTCCTGGATCATCTGCCGCATGACATTGAAGGCCACATTATCGATCTGGGCTGCGGTAAC
GGTGTCATCGGCATGGCCGCATTGATGCAGAACCCGCAAGCGCAGGTGAGCTTTGTTGATGAGTCCTATATGGCGGTGGC
CTCCAGTGAACTGAACGTGGAGCATAACCTGCCGCAGGATATGGATCGTTGCCAGTTTGAAGTGAACAACTCGCTGGCGG
GGATTGAGCGTGAAAGCGTGCAGGCGGTGCTGTGTAACCCACCGTTCCACCAGCAGCATGCCATCACCGATCACACCGCC
TGGCAGATGTTCTGTGACGCCAAGCGTTGCCTGCAGGTGGGCGGCGAGCTGCGCATCGTCGGCAACCGCCATCTGGATTA
CCACCAGAAGCTGAAGCGTTTGTTCGGTAACTGCACCCTGATCGCATCCAACAAAAAGTTCGTGATCCTCAAAGCGGTGA
AATCCGGCGCACGTTACTGA

Upstream 100 bases:

>100_bases
TCCGCTATGCGAGTGAATGCGCAATTTATCCGAAAAAACATTTTACTGGCGGACGTTTTTAGGGGATAAACAGCCCAAAT
TTTATCAGTCAGGAGGGGCA

Downstream 100 bases:

>100_bases
TTGGCGGAGCGTAGAGCCAGCCATTTCCCATCAATATCTCGTTGAATCTCTTTGGCTAATGCGGTTGGTAACCCGACCGT
ATTAGCCTGTTGTTGGAAAT

Product: rRNA (guanine-N(2)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSQLDLGTQQLELERYPQQEESTQLQAWEAADEYLLQQLENVNIGDRPVLIFNDNFGTLACALHSHQPYSISDSYMSQLA
TRHNLKLNELDPQQVTLLDTLAELPASPAVVLIRIPKALALLEQQLRALRDVVAPDTVIIAGAKARDVHTSTMQLFEKVL
GPTRTSLAWKKARLIHCEVADIVPPAAPVTTNWVLDGTDWVIHNHANVFSRSNLDIGARLFLDHLPHDIEGHIIDLGCGN
GVIGMAALMQNPQAQVSFVDESYMAVASSELNVEHNLPQDMDRCQFEVNNSLAGIERESVQAVLCNPPFHQQHAITDHTA
WQMFCDAKRCLQVGGELRIVGNRHLDYHQKLKRLFGNCTLIASNKKFVILKAVKSGARY

Sequences:

>Translated_379_residues
MSQLDLGTQQLELERYPQQEESTQLQAWEAADEYLLQQLENVNIGDRPVLIFNDNFGTLACALHSHQPYSISDSYMSQLA
TRHNLKLNELDPQQVTLLDTLAELPASPAVVLIRIPKALALLEQQLRALRDVVAPDTVIIAGAKARDVHTSTMQLFEKVL
GPTRTSLAWKKARLIHCEVADIVPPAAPVTTNWVLDGTDWVIHNHANVFSRSNLDIGARLFLDHLPHDIEGHIIDLGCGN
GVIGMAALMQNPQAQVSFVDESYMAVASSELNVEHNLPQDMDRCQFEVNNSLAGIERESVQAVLCNPPFHQQHAITDHTA
WQMFCDAKRCLQVGGELRIVGNRHLDYHQKLKRLFGNCTLIASNKKFVILKAVKSGARY
>Mature_378_residues
SQLDLGTQQLELERYPQQEESTQLQAWEAADEYLLQQLENVNIGDRPVLIFNDNFGTLACALHSHQPYSISDSYMSQLAT
RHNLKLNELDPQQVTLLDTLAELPASPAVVLIRIPKALALLEQQLRALRDVVAPDTVIIAGAKARDVHTSTMQLFEKVLG
PTRTSLAWKKARLIHCEVADIVPPAAPVTTNWVLDGTDWVIHNHANVFSRSNLDIGARLFLDHLPHDIEGHIIDLGCGNG
VIGMAALMQNPQAQVSFVDESYMAVASSELNVEHNLPQDMDRCQFEVNNSLAGIERESVQAVLCNPPFHQQHAITDHTAW
QMFCDAKRCLQVGGELRIVGNRHLDYHQKLKRLFGNCTLIASNKKFVILKAVKSGARY

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=378, Percent_Identity=64.8148148148148, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1790830, Length=167, Percent_Identity=31.1377245508982, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_SERP5 (A8GJW6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480534.1
- ProteinModelPortal:   A8GJW6
- SMR:   A8GJW6
- STRING:   A8GJW6
- GeneID:   5606425
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4312
- eggNOG:   COG2813
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- ProtClustDB:   CLSK2502835
- BioCyc:   SPRO399741:SPRO_4312-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 42283; Mature: 42152

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLDLGTQQLELERYPQQEESTQLQAWEAADEYLLQQLENVNIGDRPVLIFNDNFGTLA
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
CALHSHQPYSISDSYMSQLATRHNLKLNELDPQQVTLLDTLAELPASPAVVLIRIPKALA
EEEECCCCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHH
LLEQQLRALRDVVAPDTVIIAGAKARDVHTSTMQLFEKVLGPTRTSLAWKKARLIHCEVA
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHEEEEEEHH
DIVPPAAPVTTNWVLDGTDWVIHNHANVFSRSNLDIGARLFLDHLPHDIEGHIIDLGCGN
HCCCCCCCCEEEEEECCCCEEEECCCCHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCC
GVIGMAALMQNPQAQVSFVDESYMAVASSELNVEHNLPQDMDRCQFEVNNSLAGIERESV
CHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
QAVLCNPPFHQQHAITDHTAWQMFCDAKRCLQVGGELRIVGNRHLDYHQKLKRLFGNCTL
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCEEE
IASNKKFVILKAVKSGARY
EECCCCEEEEEEECCCCCC
>Mature Secondary Structure 
SQLDLGTQQLELERYPQQEESTQLQAWEAADEYLLQQLENVNIGDRPVLIFNDNFGTLA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
CALHSHQPYSISDSYMSQLATRHNLKLNELDPQQVTLLDTLAELPASPAVVLIRIPKALA
EEEECCCCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHH
LLEQQLRALRDVVAPDTVIIAGAKARDVHTSTMQLFEKVLGPTRTSLAWKKARLIHCEVA
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHEEEEEEHH
DIVPPAAPVTTNWVLDGTDWVIHNHANVFSRSNLDIGARLFLDHLPHDIEGHIIDLGCGN
HCCCCCCCCEEEEEECCCCEEEECCCCHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCC
GVIGMAALMQNPQAQVSFVDESYMAVASSELNVEHNLPQDMDRCQFEVNNSLAGIERESV
CHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
QAVLCNPPFHQQHAITDHTAWQMFCDAKRCLQVGGELRIVGNRHLDYHQKLKRLFGNCTL
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCEEE
IASNKKFVILKAVKSGARY
EECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA