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The map label for this gene is puuE [H]

Identifier: 157372539

GI number: 157372539

Start: 4774519

End: 4775784

Strand: Reverse

Name: puuE [H]

Synonym: Spro_4306

Alternate gene names: 157372539

Gene position: 4775784-4774519 (Counterclockwise)

Preceding gene: 157372541

Following gene: 157372538

Centisome position: 87.65

GC content: 58.53

Gene sequence:

>1266_bases
ATGAAAAACGCAGAATTGAATCAACGTCGTCAGGATGCCACCCCGCGTGGGGTAGGCGTGATGTGTGGTTTTTACGCAGA
GCGTGCAGAGAACGCCACGCTGTGGGATGTCGAAGGCAATGAAGTCATCGATTTCGCCTCTGGTATTGCCGTATTAAATA
CCGGTCACCGCCACCCGAAGGTGATCGCCGCGATTGAAAAGCAACTGAAGGCCTTCACCCACACCGCTTACCAAATTGTT
CCTTACGAAAGCTACGTCACGTTGGCGGAACGCATTAACCAGCGCGCGCCAATTGAAGGCCCATGTAAAACCGCATTCTT
CACCACCGGTGCCGAAGCGGTAGAGAACGCGGTCAAAATTGCCCGTGCTCATACCGGTCGTCCAGGTCTTATCACCTTTG
GCGGCGGTTTCCACGGCCGTACCTATATGACCATGGCGCTGACCGGCAAAGTGGCTCCTTACAAACTGGGCTTCGGGCCA
TTCCCCGGTTCGGTGTTCCACGGTCAGTACCCAAATGCGCTGTACGGCGTGACCACCGAAGACGCGATGAACAGTCTGGA
ACGTATCTTTAAAGCGGATATCGATCCCAAGCAGGTTGCGGCCATCGTGCTGGAGCCGGTGCAGGGTGAGGGTGGTTTCA
ACATCGCACCGGCCGAGTTTATGCAAGCGCTGCGCACGCTGTGTGACGAGCACGGCATTCTGCTGATTGCTGACGAAGTG
CAGACCGGTTTTGCCCGTACCGGCAAGCTGTTCGCCATGGATCATTACAGCGTGAAACCCGATCTAATCACCATGGCGAA
AAGCCTGGCGGGCGGTATGCCGCTGTCGGCGGTGGCCGGGCGTGCCGAAGTGATGGATGCTCCAGCACCGGGTGGGCTGG
GTGGCACCTATGCCGGTAACCCGCTGGCGGTGGCGGCAGCGTTGGCGGTGTTGGAAGTGATTGAAGAAGAGCAACTGTGC
CAGCGTTCGCAGCGCCTGGGGCAGCATCTGGTGGAGGTGTTGCAGCAGGCGCGCCAGACCAGCAAGGCGATCGTGGATAT
CCGCGCGCAAGGCTCGATGGTGGCAGTGGAGTTCAACGATCCGGCGACCGGCAAGCCTTCGGCGGAGATTACCCGTCAGG
TACAGCAAAAAGCGCTGGCAGAGGGCCTACTGTTGCTGAGCTGCGGCGTCAACGGCAACGTGATCCGTTTCCTGTATCCG
CTGACCATTCCTGAAGACCAGTTCACCAAGGCGCTGGGCATTCTTTCCCGCGCGCTGGCCCAATAA

Upstream 100 bases:

>100_bases
TCTCGATTATAGGGCCACATCCTGTGTAAATAAACGCCATTGTTTCACGAATGTGAACGAAACGTACAACTGGCATCGCT
CATCGACCGGAGAGAACAAC

Downstream 100 bases:

>100_bases
GGAGTGCGTGGCAATGAAATTGAATAATCCCGAGCTGCTGCGTAGCCAGTGCCTGATTAACGGTGAGTGGTGCGATGCGC
TGAGCGGCAAGCGTGAGGCG

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKNAELNQRRQDATPRGVGVMCGFYAERAENATLWDVEGNEVIDFASGIAVLNTGHRHPKVIAAIEKQLKAFTHTAYQIV
PYESYVTLAERINQRAPIEGPCKTAFFTTGAEAVENAVKIARAHTGRPGLITFGGGFHGRTYMTMALTGKVAPYKLGFGP
FPGSVFHGQYPNALYGVTTEDAMNSLERIFKADIDPKQVAAIVLEPVQGEGGFNIAPAEFMQALRTLCDEHGILLIADEV
QTGFARTGKLFAMDHYSVKPDLITMAKSLAGGMPLSAVAGRAEVMDAPAPGGLGGTYAGNPLAVAAALAVLEVIEEEQLC
QRSQRLGQHLVEVLQQARQTSKAIVDIRAQGSMVAVEFNDPATGKPSAEITRQVQQKALAEGLLLLSCGVNGNVIRFLYP
LTIPEDQFTKALGILSRALAQ

Sequences:

>Translated_421_residues
MKNAELNQRRQDATPRGVGVMCGFYAERAENATLWDVEGNEVIDFASGIAVLNTGHRHPKVIAAIEKQLKAFTHTAYQIV
PYESYVTLAERINQRAPIEGPCKTAFFTTGAEAVENAVKIARAHTGRPGLITFGGGFHGRTYMTMALTGKVAPYKLGFGP
FPGSVFHGQYPNALYGVTTEDAMNSLERIFKADIDPKQVAAIVLEPVQGEGGFNIAPAEFMQALRTLCDEHGILLIADEV
QTGFARTGKLFAMDHYSVKPDLITMAKSLAGGMPLSAVAGRAEVMDAPAPGGLGGTYAGNPLAVAAALAVLEVIEEEQLC
QRSQRLGQHLVEVLQQARQTSKAIVDIRAQGSMVAVEFNDPATGKPSAEITRQVQQKALAEGLLLLSCGVNGNVIRFLYP
LTIPEDQFTKALGILSRALAQ
>Mature_421_residues
MKNAELNQRRQDATPRGVGVMCGFYAERAENATLWDVEGNEVIDFASGIAVLNTGHRHPKVIAAIEKQLKAFTHTAYQIV
PYESYVTLAERINQRAPIEGPCKTAFFTTGAEAVENAVKIARAHTGRPGLITFGGGFHGRTYMTMALTGKVAPYKLGFGP
FPGSVFHGQYPNALYGVTTEDAMNSLERIFKADIDPKQVAAIVLEPVQGEGGFNIAPAEFMQALRTLCDEHGILLIADEV
QTGFARTGKLFAMDHYSVKPDLITMAKSLAGGMPLSAVAGRAEVMDAPAPGGLGGTYAGNPLAVAAALAVLEVIEEEQLC
QRSQRLGQHLVEVLQQARQTSKAIVDIRAQGSMVAVEFNDPATGKPSAEITRQVQQKALAEGLLLLSCGVNGNVIRFLYP
LTIPEDQFTKALGILSRALAQ

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=408, Percent_Identity=32.3529411764706, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI13994255, Length=418, Percent_Identity=30.622009569378, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI284507298, Length=323, Percent_Identity=32.1981424148607, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI37574042, Length=415, Percent_Identity=28.433734939759, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI24119277, Length=409, Percent_Identity=28.1173594132029, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI226442709, Length=388, Percent_Identity=28.8659793814433, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI226442705, Length=415, Percent_Identity=27.9518072289157, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI188536080, Length=446, Percent_Identity=26.0089686098655, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI38679950, Length=446, Percent_Identity=26.0089686098655, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI38679946, Length=446, Percent_Identity=26.0089686098655, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=72.1428571428571, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=420, Percent_Identity=55.7142857142857, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=33.9108910891089, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1788044, Length=395, Percent_Identity=35.9493670886076, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI145693181, Length=397, Percent_Identity=35.5163727959698, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786349, Length=344, Percent_Identity=33.7209302325581, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786991, Length=405, Percent_Identity=29.8765432098765, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=411, Percent_Identity=32.1167883211679, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI32564660, Length=426, Percent_Identity=31.6901408450704, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI71992977, Length=450, Percent_Identity=31.1111111111111, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25144274, Length=289, Percent_Identity=33.2179930795848, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17541228, Length=426, Percent_Identity=24.4131455399061, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323470, Length=407, Percent_Identity=33.6609336609337, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324432, Length=408, Percent_Identity=31.1274509803922, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324386, Length=318, Percent_Identity=27.3584905660377, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321456, Length=372, Percent_Identity=25.2688172043011, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357415, Length=407, Percent_Identity=32.6781326781327, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI28574759, Length=419, Percent_Identity=31.2649164677804, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI161085790, Length=417, Percent_Identity=31.4148681055156, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21356575, Length=394, Percent_Identity=29.9492385786802, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281366494, Length=376, Percent_Identity=25.2659574468085, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667139, Length=376, Percent_Identity=25.2659574468085, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667143, Length=376, Percent_Identity=25.2659574468085, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 45151; Mature: 45151

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAELNQRRQDATPRGVGVMCGFYAERAENATLWDVEGNEVIDFASGIAVLNTGHRHPK
CCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHCCEEEECCCCCCHH
VIAAIEKQLKAFTHTAYQIVPYESYVTLAERINQRAPIEGPCKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHH
ARAHTGRPGLITFGGGFHGRTYMTMALTGKVAPYKLGFGPFPGSVFHGQYPNALYGVTTE
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCEECCCCH
DAMNSLERIFKADIDPKQVAAIVLEPVQGEGGFNIAPAEFMQALRTLCDEHGILLIADEV
HHHHHHHHHHHCCCCHHHEEEHEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCH
QTGFARTGKLFAMDHYSVKPDLITMAKSLAGGMPLSAVAGRAEVMDAPAPGGLGGTYAGN
HCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCCCCCC
PLAVAAALAVLEVIEEEQLCQRSQRLGQHLVEVLQQARQTSKAIVDIRAQGSMVAVEFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECC
PATGKPSAEITRQVQQKALAEGLLLLSCGVNGNVIRFLYPLTIPEDQFTKALGILSRALA
CCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHC
Q
C
>Mature Secondary Structure
MKNAELNQRRQDATPRGVGVMCGFYAERAENATLWDVEGNEVIDFASGIAVLNTGHRHPK
CCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHCCEEEECCCCCCHH
VIAAIEKQLKAFTHTAYQIVPYESYVTLAERINQRAPIEGPCKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHH
ARAHTGRPGLITFGGGFHGRTYMTMALTGKVAPYKLGFGPFPGSVFHGQYPNALYGVTTE
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCEECCCCH
DAMNSLERIFKADIDPKQVAAIVLEPVQGEGGFNIAPAEFMQALRTLCDEHGILLIADEV
HHHHHHHHHHHCCCCHHHEEEHEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCH
QTGFARTGKLFAMDHYSVKPDLITMAKSLAGGMPLSAVAGRAEVMDAPAPGGLGGTYAGN
HCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCCCCCC
PLAVAAALAVLEVIEEEQLCQRSQRLGQHLVEVLQQARQTSKAIVDIRAQGSMVAVEFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECC
PATGKPSAEITRQVQQKALAEGLLLLSCGVNGNVIRFLYPLTIPEDQFTKALGILSRALA
CCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]