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The map label for this gene is hldE

Identifier: 157372522

GI number: 157372522

Start: 4753295

End: 4754725

Strand: Reverse

Name: hldE

Synonym: Spro_4289

Alternate gene names: 157372522

Gene position: 4754725-4753295 (Counterclockwise)

Preceding gene: 157372523

Following gene: 157372521

Centisome position: 87.26

GC content: 58.77

Gene sequence:

>1431_bases
ATGAAAGTTACACTGCCTGATTTTCGCCGCGCCGGGGTATTGGTGGTGGGTGACGTCATGTTGGATCGCTATTGGTATGG
GCCGACCAGCCGCATTTCACCGGAAGCGCCGGTGCCGGTGGTCAAGGTTGAAACCATTGAAGAGCGTCCCGGCGGTGCGG
CTAACGTAGCGATGAACATTGCTTCGCTGGGCGCCAATTCACGCCTGGTGGGCCTGACCGGTATTGATGATGCCGCGCGG
GCGCTGAGCGCCAAGCTGAATGAAGTTAACGTGCGCTGTGATTTTGTCTCGGTGCCGACGCACCCGACCATCACCAAGCT
GCGCGTGCTGTCGCGTAACCAGCAATTGATCCGCCTCGATTTCGAAGAAGGTTTCTCAAACGTCGATCCGCAGCCGCTGT
TGGAACGTATTCAGCAGGCATTGCCGCAGATTGGCGCACTGGTGCTGTCTGACTACGCCAAAGGCGCGTTGAGTCAGGTA
CAGAGCATGATCCAGCTGGCGCGTGCCGCCAAGGTGCCGGTGCTGATCGATCCAAAAGGTTCTGATTTTGAGCGTTATCG
TGGCGCAACACTGCTGACGCCAAACCTGTCCGAATTCGAAGCGGTGGTCGGTCACTGTAAAGACGAAGCCGAACTGGTTG
AGCGCGGCATGAAGCTGGTGGCGGACTTTGAGCTTTCTGCACTGCTGGTCACCCGTTCCGAGCACGGCATGACGCTGCTG
CAGCCGGGCATCGCGCCGCTGCATTTGCCCACTTTGGCGCAGGAAGTGTTTGACGTTACCGGTGCCGGCGATACCGTTAT
CGGCGTGTTGGCCGCGGCACTGGCTGCCGGCAACACGTTGGAAGAGTCCTGTTTCCTGGCCAATGCCGCCGCCGGCGTGG
TGGTAGGCAAACTGGGGACTTCGACCGTTTCACCAATCGAGCTGGAAAACGCGGTACGCGGCCGGGCCGAAACCGGCTTT
GGCGTAATGACCGAAGCCCAGTTGAAAACCGCCGTGGCGCAAGCCCGTCAGCGCGGTGAAAAAGTGGTGATGACCAACGG
CATCTTCGACATTTTGCATGCCGGTCACGTCTCTTATCTGGCCAATGCCCGCAAGCTGGGCGATCGCCTGATTGTGGCGG
TGAACAGCGATGCTTCCACTAAACGTCTGAAAGGCGAAACCCGTCCGGTCAACGCGCTGGAAAACCGCATGATCGTGCTA
GGGGCGCTGGAAGCGGTGGATTGGGTGGTGCCGTTCGAAGAAGACACGCCACAACGCCTGATCGCCGATATCCTGCCGGA
TCTGCTGGTGAAAGGCGGCGATTACAAGCCTGAAGATATCGCCGGTAGTACCGAGGTATGGGCCAATGGCGGCGACGTAA
AAGTGCTGAACTTTGAAGACGGCCTGTCGACCACCAACATCATCAAGGCAATCAAAGACGGGCGTGGCTAA

Upstream 100 bases:

>100_bases
GGGCTGAGCAGGCCGGTTGCGCAATACGTTTTGCAGCTATTACAGACGTCTGTGGTAATATCTGCGCCACTAAAATTTTG
TACTTTTTTGGAGTTATCGG

Downstream 100 bases:

>100_bases
CGTTCGCTGAAAACGGCGGTGGGCAGTGAGTGGACTGAAGCAAGAGCTGAGTTTGGCGCAGGGCGTTGGGCTATTGTCCA
CTTCACTGCTGGGTACCGGC

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVETIEERPGGAANVAMNIASLGANSRLVGLTGIDDAAR
ALSAKLNEVNVRCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFSNVDPQPLLERIQQALPQIGALVLSDYAKGALSQV
QSMIQLARAAKVPVLIDPKGSDFERYRGATLLTPNLSEFEAVVGHCKDEAELVERGMKLVADFELSALLVTRSEHGMTLL
QPGIAPLHLPTLAQEVFDVTGAGDTVIGVLAAALAAGNTLEESCFLANAAAGVVVGKLGTSTVSPIELENAVRGRAETGF
GVMTEAQLKTAVAQARQRGEKVVMTNGIFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGETRPVNALENRMIVL
GALEAVDWVVPFEEDTPQRLIADILPDLLVKGGDYKPEDIAGSTEVWANGGDVKVLNFEDGLSTTNIIKAIKDGRG

Sequences:

>Translated_476_residues
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVETIEERPGGAANVAMNIASLGANSRLVGLTGIDDAAR
ALSAKLNEVNVRCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFSNVDPQPLLERIQQALPQIGALVLSDYAKGALSQV
QSMIQLARAAKVPVLIDPKGSDFERYRGATLLTPNLSEFEAVVGHCKDEAELVERGMKLVADFELSALLVTRSEHGMTLL
QPGIAPLHLPTLAQEVFDVTGAGDTVIGVLAAALAAGNTLEESCFLANAAAGVVVGKLGTSTVSPIELENAVRGRAETGF
GVMTEAQLKTAVAQARQRGEKVVMTNGIFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGETRPVNALENRMIVL
GALEAVDWVVPFEEDTPQRLIADILPDLLVKGGDYKPEDIAGSTEVWANGGDVKVLNFEDGLSTTNIIKAIKDGRG
>Mature_476_residues
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVETIEERPGGAANVAMNIASLGANSRLVGLTGIDDAAR
ALSAKLNEVNVRCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFSNVDPQPLLERIQQALPQIGALVLSDYAKGALSQV
QSMIQLARAAKVPVLIDPKGSDFERYRGATLLTPNLSEFEAVVGHCKDEAELVERGMKLVADFELSALLVTRSEHGMTLL
QPGIAPLHLPTLAQEVFDVTGAGDTVIGVLAAALAAGNTLEESCFLANAAAGVVVGKLGTSTVSPIELENAVRGRAETGF
GVMTEAQLKTAVAQARQRGEKVVMTNGIFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGETRPVNALENRMIVL
GALEAVDWVVPFEEDTPQRLIADILPDLLVKGGDYKPEDIAGSTEVWANGGDVKVLNFEDGLSTTNIIKAIKDGRG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=477, Percent_Identity=87.2117400419287, Blast_Score=843, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_SERP5 (A8GJU3)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480511.1
- ProteinModelPortal:   A8GJU3
- SMR:   A8GJU3
- STRING:   A8GJU3
- GeneID:   5604521
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4289
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- BioCyc:   SPRO399741:SPRO_4289-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 50890; Mature: 50890

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2; PS00867 CPSASE_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVETIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHH
ASLGANSRLVGLTGIDDAARALSAKLNEVNVRCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCCEEEEEECCCHHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFSNVDPQPLLERIQQALPQIGALVLSDYAKGALSQVQSMIQLARAAKVPVLIDPKG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
SDFERYRGATLLTPNLSEFEAVVGHCKDEAELVERGMKLVADFELSALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
QPGIAPLHLPTLAQEVFDVTGAGDTVIGVLAAALAAGNTLEESCFLANAAAGVVVGKLGT
CCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCC
STVSPIELENAVRGRAETGFGVMTEAQLKTAVAQARQRGEKVVMTNGIFDILHAGHVSYL
CCCCCEEHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGETRPVNALENRMIVLGALEAVDWVVPFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCHHHHCCEEEEEEHHHHHEEECCCCCCHHHH
IADILPDLLVKGGDYKPEDIAGSTEVWANGGDVKVLNFEDGLSTTNIIKAIKDGRG
HHHHHHHHHHCCCCCCCHHCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVETIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHH
ASLGANSRLVGLTGIDDAARALSAKLNEVNVRCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCCEEEEEECCCHHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFSNVDPQPLLERIQQALPQIGALVLSDYAKGALSQVQSMIQLARAAKVPVLIDPKG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
SDFERYRGATLLTPNLSEFEAVVGHCKDEAELVERGMKLVADFELSALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
QPGIAPLHLPTLAQEVFDVTGAGDTVIGVLAAALAAGNTLEESCFLANAAAGVVVGKLGT
CCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCC
STVSPIELENAVRGRAETGFGVMTEAQLKTAVAQARQRGEKVVMTNGIFDILHAGHVSYL
CCCCCEEHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGETRPVNALENRMIVLGALEAVDWVVPFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCHHHHCCEEEEEEHHHHHEEECCCCCCHHHH
IADILPDLLVKGGDYKPEDIAGSTEVWANGGDVKVLNFEDGLSTTNIIKAIKDGRG
HHHHHHHHHHCCCCCCCHHCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA