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The map label for this gene is manX [H]

Identifier: 157372513

GI number: 157372513

Start: 4744854

End: 4745348

Strand: Reverse

Name: manX [H]

Synonym: Spro_4280

Alternate gene names: 157372513

Gene position: 4745348-4744854 (Counterclockwise)

Preceding gene: 157372514

Following gene: 157372512

Centisome position: 87.09

GC content: 54.14

Gene sequence:

>495_bases
ATGGCCATTTCATTTGTACGTATCGACGACCGGGTCATTCACGGTCAACTGGTCACCCGCTGGGCGAAGGAGTTGCCTTG
CGACGGTATTGTGGCGATCGATGATGAGGTGGCGGCAGACCCGTTGCTGTCATCGGTGATGAAGGGGGCGGTGCAGGACA
CGAAAGTGTGGCTGTTTACCGTGCAGCAGGCGATAGAAAAACTGCCGAAGGTGATCGCCAGCGACAAGCAGTACTTTGTG
ATTGGCAAGTCGCCGGTCACGCTGCGCAGGTTGATAGAGGCAGGGATCGATCTGAGTAATCGTAACAAGAAAATCAACGT
CGGGCCGATGTCTGCCCGCAAGGAAACGGTGACCATCGGCCCGAATCAATCCGTGAACGCGGCAGAGACAGAGGCCTTTG
ATTTCCTTGCTGAACAAGGACATGCCATCGAATTCCGTTTAGTCCCAGACGCCAGCCATTTCAGCTGGCTGGACGCAAAA
AAGAAACTCAAATAA

Upstream 100 bases:

>100_bases
TCACCAGGCGGCACAGGAGCTGGTAGCGCTGGCGGGTGAGGCGATTGCCGTTGCCCGTCCGGCGGTAACGGCGCAATTGG
ACGATTTCTGAGGAGAGAAG

Downstream 100 bases:

>100_bases
GCCTGAACGGCGGGAGTTAACTATGTGGTTTGAAGCGACGCTTATCGGGATACTTTGTTATCTCGGTGCGCTGAGCAGCC
CCTGGCTGCTGGGACTGACC

Product: PTS system sorbose subfamily transporter subunit IIB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAISFVRIDDRVIHGQLVTRWAKELPCDGIVAIDDEVAADPLLSSVMKGAVQDTKVWLFTVQQAIEKLPKVIASDKQYFV
IGKSPVTLRRLIEAGIDLSNRNKKINVGPMSARKETVTIGPNQSVNAAETEAFDFLAEQGHAIEFRLVPDASHFSWLDAK
KKLK

Sequences:

>Translated_164_residues
MAISFVRIDDRVIHGQLVTRWAKELPCDGIVAIDDEVAADPLLSSVMKGAVQDTKVWLFTVQQAIEKLPKVIASDKQYFV
IGKSPVTLRRLIEAGIDLSNRNKKINVGPMSARKETVTIGPNQSVNAAETEAFDFLAEQGHAIEFRLVPDASHFSWLDAK
KKLK
>Mature_163_residues
AISFVRIDDRVIHGQLVTRWAKELPCDGIVAIDDEVAADPLLSSVMKGAVQDTKVWLFTVQQAIEKLPKVIASDKQYFVI
GKSPVTLRRLIEAGIDLSNRNKKINVGPMSARKETVTIGPNQSVNAAETEAFDFLAEQGHAIEFRLVPDASHFSWLDAKK
KLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=164, Percent_Identity=29.2682926829268, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI87082217, Length=149, Percent_Identity=28.8590604026846, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18184; Mature: 18053

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISFVRIDDRVIHGQLVTRWAKELPCDGIVAIDDEVAADPLLSSVMKGAVQDTKVWLFT
CEEEEEEECCEEHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
VQQAIEKLPKVIASDKQYFVIGKSPVTLRRLIEAGIDLSNRNKKINVGPMSARKETVTIG
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEC
PNQSVNAAETEAFDFLAEQGHAIEFRLVPDASHFSWLDAKKKLK
CCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AISFVRIDDRVIHGQLVTRWAKELPCDGIVAIDDEVAADPLLSSVMKGAVQDTKVWLFT
EEEEEEECCEEHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
VQQAIEKLPKVIASDKQYFVIGKSPVTLRRLIEAGIDLSNRNKKINVGPMSARKETVTIG
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEC
PNQSVNAAETEAFDFLAEQGHAIEFRLVPDASHFSWLDAKKKLK
CCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]