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The map label for this gene is manZ [H]

Identifier: 157372511

GI number: 157372511

Start: 4743210

End: 4744031

Strand: Reverse

Name: manZ [H]

Synonym: Spro_4278

Alternate gene names: 157372511

Gene position: 4744031-4743210 (Counterclockwise)

Preceding gene: 157372512

Following gene: 157372506

Centisome position: 87.06

GC content: 55.84

Gene sequence:

>822_bases
ATGGATACGCCAACACCTAAATTGAATAAGCAGGATCTGCGTCGTTGCTGGCGCAGCTGGATGATGTACAACCTGTCATC
GATGAGTTTCGAACGCCTGGAATCTTACGGTTTTTGCCTGAGCATGATGCCGGCGCTGAAAAAACTGTATCAGGATAAAC
AGCAGCGTATTGAAGCCATTCGCCGTCATACCTCTTTCTACAATACCGAGCCACAGCTGGGCGCGATTGTTAACGGCATG
GTGATTGGGCTGGAAGAAAAACGTGCCAACGGCGAGCCGATCGACGGGGAAACCATCAACACGCTCAAGGTGGGGCTGAT
GGGGCCGGTGGCGGGTATCGGAGACTCGATGGTGCCGGGCATGCTGATCCCGATTTTGTTAAGTATCGGCATGGCGCTGG
CAGCCGGCGGCAGTGTGATTGGCCCGCTGTTTTACATCATCGCGTTTAGCCTGATCGTCATTCCGGGGTCTTACTGGCTG
TTTATGAAAGGCTACCATATGGGATCCGGCTCGGTGGAGATGCTGGTCAGCAGCAAGTCCTCACGGCTGCGGGAAGCCCT
TTCTCTGCTGGGAGTGTTCGTCATGGGCGGCGTCGCGGCGAGCTACGTCAAACTGGGGACTGGGCTGGAGTTTGTGACCA
AAGACGGGGTGAATATTCACCTGCAGCAAATGCTGGACGGCATTTTCCCTAACCTGATGCCGCTGATAGTGGTGCTGGCG
ACCTGGTATCTGATGGCCAAGCGCGGACTGTCACCGGTGAAGGCGATGCTGGGGTTGTTGGTATTGGCGGCGGCAGGGGT
GGCCTGCGGTCTATTCTCTTAA

Upstream 100 bases:

>100_bases
TCGGCGCACTGCTGGCACTTATTCACTACAACTATAAGCCAGAGCCGCAGGTGGCCGCCGCACAGGGCACGCCTCAGGCT
GATGATGAGGATGAATTCTG

Downstream 100 bases:

>100_bases
TACCCGTCGCCTTTCAAGTTGCAGCGTTGTTACCTGCACTCAGTTATGTGGCCCGTCCATGGGCCACACCCCTTTGGGGC
CGCTGCTAGCAGCGTTCAAA

Product: PTS system mannose/fructose/sorbose family transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MDTPTPKLNKQDLRRCWRSWMMYNLSSMSFERLESYGFCLSMMPALKKLYQDKQQRIEAIRRHTSFYNTEPQLGAIVNGM
VIGLEEKRANGEPIDGETINTLKVGLMGPVAGIGDSMVPGMLIPILLSIGMALAAGGSVIGPLFYIIAFSLIVIPGSYWL
FMKGYHMGSGSVEMLVSSKSSRLREALSLLGVFVMGGVAASYVKLGTGLEFVTKDGVNIHLQQMLDGIFPNLMPLIVVLA
TWYLMAKRGLSPVKAMLGLLVLAAAGVACGLFS

Sequences:

>Translated_273_residues
MDTPTPKLNKQDLRRCWRSWMMYNLSSMSFERLESYGFCLSMMPALKKLYQDKQQRIEAIRRHTSFYNTEPQLGAIVNGM
VIGLEEKRANGEPIDGETINTLKVGLMGPVAGIGDSMVPGMLIPILLSIGMALAAGGSVIGPLFYIIAFSLIVIPGSYWL
FMKGYHMGSGSVEMLVSSKSSRLREALSLLGVFVMGGVAASYVKLGTGLEFVTKDGVNIHLQQMLDGIFPNLMPLIVVLA
TWYLMAKRGLSPVKAMLGLLVLAAAGVACGLFS
>Mature_273_residues
MDTPTPKLNKQDLRRCWRSWMMYNLSSMSFERLESYGFCLSMMPALKKLYQDKQQRIEAIRRHTSFYNTEPQLGAIVNGM
VIGLEEKRANGEPIDGETINTLKVGLMGPVAGIGDSMVPGMLIPILLSIGMALAAGGSVIGPLFYIIAFSLIVIPGSYWL
FMKGYHMGSGSVEMLVSSKSSRLREALSLLGVFVMGGVAASYVKLGTGLEFVTKDGVNIHLQQMLDGIFPNLMPLIVVLA
TWYLMAKRGLSPVKAMLGLLVLAAAGVACGLFS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=281, Percent_Identity=36.2989323843416, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789529, Length=268, Percent_Identity=31.3432835820896, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 29694; Mature: 29694

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPTPKLNKQDLRRCWRSWMMYNLSSMSFERLESYGFCLSMMPALKKLYQDKQQRIEAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRHTSFYNTEPQLGAIVNGMVIGLEEKRANGEPIDGETINTLKVGLMGPVAGIGDSMVPG
HHHHHHCCCCCHHHHHHHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHCCHHCCCCHHHHH
MLIPILLSIGMALAAGGSVIGPLFYIIAFSLIVIPGSYWLFMKGYHMGSGSVEMLVSSKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECEECCCCCHHHHHHHHH
SRLREALSLLGVFVMGGVAASYVKLGTGLEFVTKDGVNIHLQQMLDGIFPNLMPLIVVLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCEEHHHHHHHHCHHHHHHHHHHH
TWYLMAKRGLSPVKAMLGLLVLAAAGVACGLFS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTPTPKLNKQDLRRCWRSWMMYNLSSMSFERLESYGFCLSMMPALKKLYQDKQQRIEAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRHTSFYNTEPQLGAIVNGMVIGLEEKRANGEPIDGETINTLKVGLMGPVAGIGDSMVPG
HHHHHHCCCCCHHHHHHHHHHEEEHHHCCCCCCCCCCCHHHHHHHHHCCHHCCCCHHHHH
MLIPILLSIGMALAAGGSVIGPLFYIIAFSLIVIPGSYWLFMKGYHMGSGSVEMLVSSKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECEECCCCCHHHHHHHHH
SRLREALSLLGVFVMGGVAASYVKLGTGLEFVTKDGVNIHLQQMLDGIFPNLMPLIVVLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCEEHHHHHHHHCHHHHHHHHHHH
TWYLMAKRGLSPVKAMLGLLVLAAAGVACGLFS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]