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The map label for this gene is outF [H]

Identifier: 157372482

GI number: 157372482

Start: 4710316

End: 4711527

Strand: Reverse

Name: outF [H]

Synonym: Spro_4249

Alternate gene names: 157372482

Gene position: 4711527-4710316 (Counterclockwise)

Preceding gene: 157372483

Following gene: 157372481

Centisome position: 86.47

GC content: 57.01

Gene sequence:

>1212_bases
GTGAAGTTTAACTATCACGCCTACGATGCCGCCGGCAAAGGCGTCAGAGGGGTACTGGAGACGGATTCTTTGCGCAGTGC
CCGGCAACGGCTCCGTGAGCAAAACCTGACGCCCTCGCATATTGCGCCGCAGGGAAACAGCGTGGCCGCCACTTTGCTGG
CCGCTCGGCGCCGACATCGTTTGAGCAGTGGCGAACTGGCTCTGGTGACCCGGCAGCTCGCCACCCTGATCGCGGCGGCT
TTGCCACTGGAAGAGGCACTGTTAACCGTCGCATGTCAGTCAGAAAAAAAAACCGTTATCCAGTTAATCACCCGCATACG
CAGCCATGTGCTGGAAGGCTTGTCGTTGTCGCAGGCATTGATGCAATTTCCCTCGGTTTTTGACCGTCTGTTTTGCGCCA
TGGTGGCCGCCGGAGAGAGCAGCGGCCAATTGCCGCTGATCTTCACCGAGTTGGCGGACTATGTCGAACAGCGGCAGAAA
ATCAAAAATAAAATGACTCAGGCAATGACTTACCCCGTGATATTGACGCTGGTCGCCATTGCGGTGATCGGCGTGCTGTT
GGTGGCGGTGGTACCGAAGGTAATTGAACAGTTTGTGCATATGAAACAAACGCTGCCGTTAACCACGCAGATCCTCCTGC
TGTGCAGTGAAGGCGTCGGTCGGTGGGGGCCTTCTGGCCTGGCGGTGGTCGTGCTGACCTTGCTGGGTTTTCGCTGGCAG
TTGAAAAAACAGCGGTTTCGCCTGGCGTGGCACCGTTATCTGTTGCGCATGCCGTACCTGGGAAGCCTGTTCCTGAATCT
GGATACGGCCCGTTATGCACACACTCTCAGCATCCTGACGCTTAGCGCGGTGCCTTTATTGGACAGCATGAATATCAGTG
CTGCGGTGTTGAATAACGAGGAGGCCCGGCGTCGGTTGCTCGAGGCGATGACGCAGGTACAGGAGGGGGGATCTTTGCAT
CAGGCGCTGGCGGACAGCGATCTTTTTTCCCCGATGATGCGCCACATGATCGCGGCGGGCGAACGCAGCAGTGAACTTGG
CAGCATGTTGAAACGGGCCGCCGCTATGCAACAGGACGCCCTTAATCAGCGCATTGCGTTGGTCCTGAGCCTGTTTGAGC
CGGCGCTGGTGATCAGCATGGCCGGCATTGTCCTGTTCATTATTCTGGCGATCCTGCAGCCGATCTTACAGCTCAACAAC
ATGATGGGTTAA

Upstream 100 bases:

>100_bases
CATGCGCGACACACCCTGCGGCAAGATGGTGCGCGCAAACTGCTGACCGGTGAAACCAGTATTGAGGAAGTTCTGCGGGT
CACCGGGGGGGATCATTGCG

Downstream 100 bases:

>100_bases
CTCAATGGAGAACCGTATGCGAACCCCAACAATGGCAAGGAACACCCGGCAGTCGGGATTTACACTGATGGAAATCATGG
TGGTGATTGTGATCCTGGGC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKFNYHAYDAAGKGVRGVLETDSLRSARQRLREQNLTPSHIAPQGNSVAATLLAARRRHRLSSGELALVTRQLATLIAAA
LPLEEALLTVACQSEKKTVIQLITRIRSHVLEGLSLSQALMQFPSVFDRLFCAMVAAGESSGQLPLIFTELADYVEQRQK
IKNKMTQAMTYPVILTLVAIAVIGVLLVAVVPKVIEQFVHMKQTLPLTTQILLLCSEGVGRWGPSGLAVVVLTLLGFRWQ
LKKQRFRLAWHRYLLRMPYLGSLFLNLDTARYAHTLSILTLSAVPLLDSMNISAAVLNNEEARRRLLEAMTQVQEGGSLH
QALADSDLFSPMMRHMIAAGERSSELGSMLKRAAAMQQDALNQRIALVLSLFEPALVISMAGIVLFIILAILQPILQLNN
MMG

Sequences:

>Translated_403_residues
MKFNYHAYDAAGKGVRGVLETDSLRSARQRLREQNLTPSHIAPQGNSVAATLLAARRRHRLSSGELALVTRQLATLIAAA
LPLEEALLTVACQSEKKTVIQLITRIRSHVLEGLSLSQALMQFPSVFDRLFCAMVAAGESSGQLPLIFTELADYVEQRQK
IKNKMTQAMTYPVILTLVAIAVIGVLLVAVVPKVIEQFVHMKQTLPLTTQILLLCSEGVGRWGPSGLAVVVLTLLGFRWQ
LKKQRFRLAWHRYLLRMPYLGSLFLNLDTARYAHTLSILTLSAVPLLDSMNISAAVLNNEEARRRLLEAMTQVQEGGSLH
QALADSDLFSPMMRHMIAAGERSSELGSMLKRAAAMQQDALNQRIALVLSLFEPALVISMAGIVLFIILAILQPILQLNN
MMG
>Mature_403_residues
MKFNYHAYDAAGKGVRGVLETDSLRSARQRLREQNLTPSHIAPQGNSVAATLLAARRRHRLSSGELALVTRQLATLIAAA
LPLEEALLTVACQSEKKTVIQLITRIRSHVLEGLSLSQALMQFPSVFDRLFCAMVAAGESSGQLPLIFTELADYVEQRQK
IKNKMTQAMTYPVILTLVAIAVIGVLLVAVVPKVIEQFVHMKQTLPLTTQILLLCSEGVGRWGPSGLAVVVLTLLGFRWQ
LKKQRFRLAWHRYLLRMPYLGSLFLNLDTARYAHTLSILTLSAVPLLDSMNISAAVLNNEEARRRLLEAMTQVQEGGSLH
QALADSDLFSPMMRHMIAAGERSSELGSMLKRAAAMQQDALNQRIALVLSLFEPALVISMAGIVLFIILAILQPILQLNN
MMG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=48.6352357320099, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1786295, Length=412, Percent_Identity=26.4563106796117, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44465; Mature: 44465

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNYHAYDAAGKGVRGVLETDSLRSARQRLREQNLTPSHIAPQGNSVAATLLAARRRHR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
LSSGELALVTRQLATLIAAALPLEEALLTVACQSEKKTVIQLITRIRSHVLEGLSLSQAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHH
MQFPSVFDRLFCAMVAAGESSGQLPLIFTELADYVEQRQKIKNKMTQAMTYPVILTLVAI
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVIGVLLVAVVPKVIEQFVHMKQTLPLTTQILLLCSEGVGRWGPSGLAVVVLTLLGFRWQ
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LKKQRFRLAWHRYLLRMPYLGSLFLNLDTARYAHTLSILTLSAVPLLDSMNISAAVLNNE
HHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCH
EARRRLLEAMTQVQEGGSLHQALADSDLFSPMMRHMIAAGERSSELGSMLKRAAAMQQDA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LNQRIALVLSLFEPALVISMAGIVLFIILAILQPILQLNNMMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]