Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 157372349

GI number: 157372349

Start: 4569495

End: 4570850

Strand: Reverse

Name: dnaB [H]

Synonym: Spro_4115

Alternate gene names: 157372349

Gene position: 4570850-4569495 (Counterclockwise)

Preceding gene: 157372350

Following gene: 157372348

Centisome position: 83.89

GC content: 54.35

Gene sequence:

>1356_bases
ATGAACCAGACAACAACCAATATTCCTTATTCCTCCGATGCTGAGCAGGCCGTTCTCGGCGGCTTGATGCTGGATAACGA
GCGGTGGGATGACGTTCTCCCATTGGTTGGCGAGCGTGATTTTCATCTGGCGGTTCATCGCCGAATTTTCCAGGCCATGG
CTCGCCTGATGGCTGTTCAACAGCCGATTGATCTGATTACGCTTCATGAATCACTGGAGCAAAATGGTGCGCTTGAACAG
GTGGGTGGTTTTGCTTATCTGGCGGAATTATCTAAAAACACGCCGAGCGTAGCCAATATTGTCGCCTATGCCGAGATTGT
GGCTGAACGCAGTCGTCTGCGCAGCCTGTTGCAACTCGGGCGTGAATTGAGCGCTGATGCCGCCAATCCCCGCGCAGAGT
CTGCGGCCCTGACCGAGCGGGCTGAGCAGCAATTGTTTCAGATCTCTGAGCAAACCCAGTCACAGCAGAATGTCAGCCTG
ACGGATGGGTTGAATGCTGTTGTGACCTATCTGGAGACGGTGAATGGCGGCAATGGTATCACCGGCACCCCGACCGGTTT
CTCACAGCTTGATGAGATGACGTGTGGTTTGCAGCCCGGAGATTTGGTTTTGCTTGCGGCACGGCCTTCGATGGGGAAAA
CCGCCTTTGCGTTGGGCGCCTACACTGGCGCGCTTCGTGGTACGGAGAAACCTGCATTCTTCTTCAGTCTGGAAATGCCG
CGTGAGCAACTGCTTCAACGTCTCATTGCTGTAGACGGAAGGGTTGAACTTTCACGTCTGCGCAGCGGCCAGTTGGATGA
TGAAGATTGGGCGCGTATTTCCAAAGCGCTGGAACGGTTATTGCCATTGGAAAACCGCCTGATTATTGACGATGAATCAT
ATTTGACCCCCGCACTGTTGCGTTCCAGGGCTCGCCGCTACACTCGCTTGTACGGTAAGCCGGGTTTAATCATGGTCGAT
TACCTACAGTTGATGCGTTGCCCAGGGCAGGAAAACCGCACCCAGGAAATTGCGGAAATTTCCCGCAGCCTGAAAGCGTT
GGCCAAAGAACTCGACTGTCCCGTCGTCGCGCTGTCTCAGTTGAACCGTCAGTTGGAGAGCCGTGCGGATAAGCGTCCGT
TCAATGGCGATCTGCGCGATTCCGGTGCGCTGGAGCAGGATGCGGATGTGATCGCGTTCATCTACCGTGATGAAGTTTAT
TACGCTGATTCAGATGATGCCGGCACGGCAGAAATTATTATTGGTAAGCAGAGGCAGGGGGCAACGGGTACCGTTCGAGT
CAAGTTTGAAGGGCAATTCACCGCGTTCAGCGACCTCCACGACGGTCAGTATGGCCAATACTCGGGGAGGGCGTAA

Upstream 100 bases:

>100_bases
TGGCAGCTGCTGACACCATGCATATGCTGGCTTGTGAACTGTTCCCTCAGTGGAAATCAGACTTTGATGCACTACTGGCC
GGGAAGTCGGAGGTGCAGCT

Downstream 100 bases:

>100_bases
CCATGGCGCGTGCTAAAACCAAGCTCCTTGATCTGAACACTGCTTTGCTCCAGCAGGGAAAAACCGCTGTCGGCACTCAA
GCATCAACCTCGACAATGTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNQTTTNIPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQQPIDLITLHESLEQNGALEQ
VGGFAYLAELSKNTPSVANIVAYAEIVAERSRLRSLLQLGRELSADAANPRAESAALTERAEQQLFQISEQTQSQQNVSL
TDGLNAVVTYLETVNGGNGITGTPTGFSQLDEMTCGLQPGDLVLLAARPSMGKTAFALGAYTGALRGTEKPAFFFSLEMP
REQLLQRLIAVDGRVELSRLRSGQLDDEDWARISKALERLLPLENRLIIDDESYLTPALLRSRARRYTRLYGKPGLIMVD
YLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQLNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVY
YADSDDAGTAEIIIGKQRQGATGTVRVKFEGQFTAFSDLHDGQYGQYSGRA

Sequences:

>Translated_451_residues
MNQTTTNIPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQQPIDLITLHESLEQNGALEQ
VGGFAYLAELSKNTPSVANIVAYAEIVAERSRLRSLLQLGRELSADAANPRAESAALTERAEQQLFQISEQTQSQQNVSL
TDGLNAVVTYLETVNGGNGITGTPTGFSQLDEMTCGLQPGDLVLLAARPSMGKTAFALGAYTGALRGTEKPAFFFSLEMP
REQLLQRLIAVDGRVELSRLRSGQLDDEDWARISKALERLLPLENRLIIDDESYLTPALLRSRARRYTRLYGKPGLIMVD
YLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQLNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVY
YADSDDAGTAEIIIGKQRQGATGTVRVKFEGQFTAFSDLHDGQYGQYSGRA
>Mature_451_residues
MNQTTTNIPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQQPIDLITLHESLEQNGALEQ
VGGFAYLAELSKNTPSVANIVAYAEIVAERSRLRSLLQLGRELSADAANPRAESAALTERAEQQLFQISEQTQSQQNVSL
TDGLNAVVTYLETVNGGNGITGTPTGFSQLDEMTCGLQPGDLVLLAARPSMGKTAFALGAYTGALRGTEKPAFFFSLEMP
REQLLQRLIAVDGRVELSRLRSGQLDDEDWARISKALERLLPLENRLIIDDESYLTPALLRSRARRYTRLYGKPGLIMVD
YLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQLNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVY
YADSDDAGTAEIIIGKQRQGATGTVRVKFEGQFTAFSDLHDGQYGQYSGRA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=442, Percent_Identity=55.6561085972851, Blast_Score=465, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49923; Mature: 49923

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTTTNIPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQ
CCCCCCCCCCCCCHHHHHHCCEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QPIDLITLHESLEQNGALEQVGGFAYLAELSKNTPSVANIVAYAEIVAERSRLRSLLQLG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RELSADAANPRAESAALTERAEQQLFQISEQTQSQQNVSLTDGLNAVVTYLETVNGGNGI
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
TGTPTGFSQLDEMTCGLQPGDLVLLAARPSMGKTAFALGAYTGALRGTEKPAFFFSLEMP
CCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
REQLLQRLIAVDGRVELSRLRSGQLDDEDWARISKALERLLPLENRLIIDDESYLTPALL
HHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
RSRARRYTRLYGKPGLIMVDYLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQ
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVYYADSDDAGTAEIIIGKQRQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCCCCCCEEEEEECCCCC
ATGTVRVKFEGQFTAFSDLHDGQYGQYSGRA
CCCEEEEEECCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQTTTNIPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQ
CCCCCCCCCCCCCHHHHHHCCEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QPIDLITLHESLEQNGALEQVGGFAYLAELSKNTPSVANIVAYAEIVAERSRLRSLLQLG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RELSADAANPRAESAALTERAEQQLFQISEQTQSQQNVSLTDGLNAVVTYLETVNGGNGI
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
TGTPTGFSQLDEMTCGLQPGDLVLLAARPSMGKTAFALGAYTGALRGTEKPAFFFSLEMP
CCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
REQLLQRLIAVDGRVELSRLRSGQLDDEDWARISKALERLLPLENRLIIDDESYLTPALL
HHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
RSRARRYTRLYGKPGLIMVDYLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQ
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVYYADSDDAGTAEIIIGKQRQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCCCCCCEEEEEECCCCC
ATGTVRVKFEGQFTAFSDLHDGQYGQYSGRA
CCCEEEEEECCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]