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The map label for this gene is mscS [H]

Identifier: 157372176

GI number: 157372176

Start: 4366661

End: 4367527

Strand: Reverse

Name: mscS [H]

Synonym: Spro_3942

Alternate gene names: 157372176

Gene position: 4367527-4366661 (Counterclockwise)

Preceding gene: 157372178

Following gene: 157372162

Centisome position: 80.15

GC content: 56.52

Gene sequence:

>867_bases
ATGAAAGATCTGAATGTAGTAGACGGCATTAATGGTGCCGGTAACTGGCTGGTGCAGAACCAGGATTTATTGATTCAGTA
TGCGGTGAACATCGTCGCGGCGATTGTTATTTTCATCATTGGTTCGATCATTGCCCGAGTAGTCAGCAATGCGGTAAACC
GCGTGATGAAGCTGCGCGGCATCGATGCCACGGTAGCCGATTTCCTGTCGGCCATGGCGCGTTACGGCATTTTGGCCTTC
ACCTTTATTGCAGTGCTGGGGCGCGTAGGCGTACAGACTACCTCGGTGATCGCCGTGCTGGGTGCCGCCGGTTTGGCCGT
CGGCCTGGCGCTGCAGGGCTCGCTGTCCAACTTCGCCGCAGGCGTGCTGCTGGTGGCCTTCCGTCCACTGCGTGCCGGTG
AGTATGTCGATCTGGGCGGCGTAGCCGGTACCGTCGACCAGGTGCAGATTTTCTCCACCACGTTGCGCACCGCAGACAAC
AAAACCATCGTGGTACCGAACGGTAAAATCATCGCCGGCAACATCATCAACTATTCCCGCGAGCCGAACCGCCGTGTGGA
TATCGTGGTTGGCGTGGCTTATAGCGCCGACATCGATGTGGTGAAAAAGGTACTGGGCGATGTGATCGCCGCTGACAAGC
GCATCATGCACGCCAAAGGCGTGACCGTGCGTCTGAACGAGATGGCGCCTTCTTCACTGAACTTCGTGACCCGCTCCTGG
ACCACTAATGCCGAGTATTGGAACGTGTACTTCGATCTGATGGAGAACTTCAAGCGCGCGCTGGATGCCAACAACATCGG
TATTCCGTTCCCGCAGATGGACGTTCATCTGTATCGCAACGATGCGACCACCGCGAAAGCGGAATAA

Upstream 100 bases:

>100_bases
GCGGAAATGTTAGTTAACCTAGGGCGGTGGTTTCCGACTGACGCCAGATGTCAGTTTTTTTGCGAGTTTATCCCCTTAGG
GAAAAGTTAGGACGACACGA

Downstream 100 bases:

>100_bases
CGGAAAGGGGCCTTGGCCCCTTTTTTATTTGTTCAGTTGGGCAACGCAGGGTCATGCGCAGATGCCCGGCGGCTCAAACT
GAGCCGGTAGAGCAGGAAGA

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKDLNVVDGINGAGNWLVQNQDLLIQYAVNIVAAIVIFIIGSIIARVVSNAVNRVMKLRGIDATVADFLSAMARYGILAF
TFIAVLGRVGVQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVAFRPLRAGEYVDLGGVAGTVDQVQIFSTTLRTADN
KTIVVPNGKIIAGNIINYSREPNRRVDIVVGVAYSADIDVVKKVLGDVIAADKRIMHAKGVTVRLNEMAPSSLNFVTRSW
TTNAEYWNVYFDLMENFKRALDANNIGIPFPQMDVHLYRNDATTAKAE

Sequences:

>Translated_288_residues
MKDLNVVDGINGAGNWLVQNQDLLIQYAVNIVAAIVIFIIGSIIARVVSNAVNRVMKLRGIDATVADFLSAMARYGILAF
TFIAVLGRVGVQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVAFRPLRAGEYVDLGGVAGTVDQVQIFSTTLRTADN
KTIVVPNGKIIAGNIINYSREPNRRVDIVVGVAYSADIDVVKKVLGDVIAADKRIMHAKGVTVRLNEMAPSSLNFVTRSW
TTNAEYWNVYFDLMENFKRALDANNIGIPFPQMDVHLYRNDATTAKAE
>Mature_288_residues
MKDLNVVDGINGAGNWLVQNQDLLIQYAVNIVAAIVIFIIGSIIARVVSNAVNRVMKLRGIDATVADFLSAMARYGILAF
TFIAVLGRVGVQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVAFRPLRAGEYVDLGGVAGTVDQVQIFSTTLRTADN
KTIVVPNGKIIAGNIINYSREPNRRVDIVVGVAYSADIDVVKKVLGDVIAADKRIMHAKGVTVRLNEMAPSSLNFVTRSW
TTNAEYWNVYFDLMENFKRALDANNIGIPFPQMDVHLYRNDATTAKAE

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=279, Percent_Identity=69.1756272401434, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786670, Length=212, Percent_Identity=28.7735849056604, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787591, Length=247, Percent_Identity=24.6963562753036, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI2367355, Length=178, Percent_Identity=24.1573033707865, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30954; Mature: 30954

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLNVVDGINGAGNWLVQNQDLLIQYAVNIVAAIVIFIIGSIIARVVSNAVNRVMKLRG
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IDATVADFLSAMARYGILAFTFIAVLGRVGVQTTSVIAVLGAAGLAVGLALQGSLSNFAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GVLLVAFRPLRAGEYVDLGGVAGTVDQVQIFSTTLRTADNKTIVVPNGKIIAGNIINYSR
HHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEECCEECCCC
EPNRRVDIVVGVAYSADIDVVKKVLGDVIAADKRIMHAKGVTVRLNEMAPSSLNFVTRSW
CCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCCCHHEEEECC
TTNAEYWNVYFDLMENFKRALDANNIGIPFPQMDVHLYRNDATTAKAE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MKDLNVVDGINGAGNWLVQNQDLLIQYAVNIVAAIVIFIIGSIIARVVSNAVNRVMKLRG
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IDATVADFLSAMARYGILAFTFIAVLGRVGVQTTSVIAVLGAAGLAVGLALQGSLSNFAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GVLLVAFRPLRAGEYVDLGGVAGTVDQVQIFSTTLRTADNKTIVVPNGKIIAGNIINYSR
HHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEECCEECCCC
EPNRRVDIVVGVAYSADIDVVKKVLGDVIAADKRIMHAKGVTVRLNEMAPSSLNFVTRSW
CCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCCCHHEEEECC
TTNAEYWNVYFDLMENFKRALDANNIGIPFPQMDVHLYRNDATTAKAE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]