Click here to switch to the map view.

The map label for this gene is ptsP [H]

Identifier: 157372058

GI number: 157372058

Start: 4229884

End: 4232130

Strand: Reverse

Name: ptsP [H]

Synonym: Spro_3823

Alternate gene names: 157372058

Gene position: 4232130-4229884 (Counterclockwise)

Preceding gene: 157372059

Following gene: 157372057

Centisome position: 77.67

GC content: 58.79

Gene sequence:

>2247_bases
ATGCTCACGCGCTTGCGAGAAATTGTTGAGAAGGTGGCCGCGGCGGCCAGTCTGACAGATGCGCTGGATCTTTTGGTCAA
TGAAACCTGTCTGGCAATGGATACAGAAGTCTGTTCCATCTACCTGGCGGACAACGACCGCCGCTGCTATTACCTGATGG
CGACACGCGGGTTAAAAAAACCGCGTGGGCGCGCTATCGCACTGGCATTTGATGAAGGTATTGTCGGATTGGTCGGACGC
CGTGCGGAGCCGATTAACCTGGCAGACGCGCAGAGCCACCCCAGCTTCAAATACATTCCCCAGGTGAAAGAGGATCGTTT
CCGATCCTTCCTCGGCGTGCCGATCATTCATCGCCGTCAGTTGCTGGGGGTATTGGTGGTGCAGCAGCGTGAACTGCGCC
AGTTTGACGAAAGCGAAGAATCCTTCATGGTGACCCTGGCCACGCAAATGGCCGGGATCCTCTCGCAGTCACAGCTTAAC
GCCATTTTTGGTCAATATCGTCAGACGCGTATTCGTGCACTGGCGGCATCGCCGGGCGTCGCGGTAGCTGAAGGCTGGCA
GGACAGCAGCCAGCCGTCTCTCGACCAGGTTTACAAAGCCTCGACGCTCGATACCGCCAGCGAACGTGAACGCCTGACTC
AGGCACTCGAAGAGGCCGGCGCGGAATTCCGCCGTTTCAGCAAGCGCTTTGCCGCCAGCTCGCAAAAAGAGAGCGCGGCG
ATCTTCGATCTTTACTCTCACCTGCTCAACGACGCTCGCCTCAAGCGCGAACTCTTTGCCGAGATTGATGCCGGTTCCGT
GGCGGAATGGGCGGTTAAACAGGTGATCGAAGTCTTTGCCGCCCAGTTTGCCAGCCTGCAAGACACCTATATGCGTGAGC
GTGCCAGCGATCTGCGCGCGCTCGGCCAGCGTCTGCTATTCCATCTCGATGACACCACCCAGGGCGCTACCCAGTGGCCG
GCGCGTTTTGTGCTGGTAGCCGATGAGCTGACCGCGACTCTGCTGGCTGAAATGCCGCAGGACAGGCTGGTCGGCGTGGT
GGTGCGCGACGGTGCCGCCAACTCCCATGCAGCCATATTGGTGCGGGCGATGGGCGTACCGACGGTGATGGGCGCGGATA
TTCAGCCTGCGCTGCTCAGCCAGCGACTGCTGATTGTCGATGGTTATCGCGGCGAACTTTTGGTCGACCCCGAACCGGTG
CTGGTGCAGGAATACCAACGGCTGATCAGCGAAGAGCTGGAATTAAGCCGGTTGGCGGAAGATGACGTTGAGCAACCGGC
CCAGCTTAAAAGCGGTGAGCGAGTTCAGGTGATGCTTAATGCCGGCCTCAGCCCGGAACATGAAAAGCTGCTTGGCGGGC
GGGTTGACGGTGTGGGTCTGTACCGTACCGAAATTCCCTTTATGCTACAGAGCGGCTTCCCTTCCGAAGAGGAGCAGGTT
GCCCAGTATCAGGGCATGTTGCAGATGTACCCGAATAAGCCAGTGACGCTACGTACGCTGGATATCGGTGCCGATAAACA
ACTGCCTTATATGCCGATCAGCGAAGAAAACCCGTGCCTGGGCTGGCGTGGTATCCGAATTACCCTGGATCAACCGGAGA
TCTTCCTGATCCAGGTGCGAGCCATGCTGCGCGCCAATGCCGGTACCGGCAATCTGGGCATACTGCTGCCGATGATCACC
AGCCTGGAAGAGATCGACGAGGCTCGCAGGTTGATCGACCGGGCAGGGCGGGAAGTGGAAGAGGTGCTGGGGTATGAAAT
CCCAAAACCCAAATTGGGCATCATGCTGGAAGTCCCTTCGATGATCTTCATGATCCCGCATCTGGCCGGGCGTATTGAGT
TTATTTCGGTGGGGACCAACGACCTGACCCAATACCTGCTGGCGGTGGATCGCAACAATACCCGGGTGGCCTCACTGTAC
GACAGCCTGCATCCTTCCATGTTGCAGGTGCTGAAGCTGATAGCCGTTCAGGCCAGGGCCGTCGGGCTGCAGGTCAGCCT
GTGTGGTGAACTGGCCGGCGACCCAATGGGGGCGCTGCTGCTGGTGGGGATGGGCTACCGCAACCTGAGCATGAACGGCC
GCAGCGTGGCGCGGATAAAATATCTGCTGCGACACATTGAGTTATCCGACGCCGAAGTGCTGGCCCAGCGGGTGCTTAAC
GCCCAAATGACCACCGAAGTGCGCCATCAGGTGGCGGCATTTATGGAACGTCGTGGTATGGGCGGTTTGATCCGCGGCGG
CAAGTAA

Upstream 100 bases:

>100_bases
GATGAAAGAATTCGCCGTCACCGTGATGCCGATGCAGGAGCAGGCAGCACAGCGGCAGACCCCCGCTTATCGCCGGAAGA
GAGGTTAAGTTAAGCCGACT

Downstream 100 bases:

>100_bases
GGCCGCGGGCTGACTGATTTTTTTACAGAACTTTTCCCGGCGGGCCAGTCCGGTGGTGGTTAAGCTTATGCTATTATCCG
CTTCCTCAACTGCGTTGAAA

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVAAAASLTDALDLLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKKPRGRAIALAFDEGIVGLVGR
RAEPINLADAQSHPSFKYIPQVKEDRFRSFLGVPIIHRRQLLGVLVVQQRELRQFDESEESFMVTLATQMAGILSQSQLN
AIFGQYRQTRIRALAASPGVAVAEGWQDSSQPSLDQVYKASTLDTASERERLTQALEEAGAEFRRFSKRFAASSQKESAA
IFDLYSHLLNDARLKRELFAEIDAGSVAEWAVKQVIEVFAAQFASLQDTYMRERASDLRALGQRLLFHLDDTTQGATQWP
ARFVLVADELTATLLAEMPQDRLVGVVVRDGAANSHAAILVRAMGVPTVMGADIQPALLSQRLLIVDGYRGELLVDPEPV
LVQEYQRLISEELELSRLAEDDVEQPAQLKSGERVQVMLNAGLSPEHEKLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMYPNKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAGTGNLGILLPMIT
SLEEIDEARRLIDRAGREVEEVLGYEIPKPKLGIMLEVPSMIFMIPHLAGRIEFISVGTNDLTQYLLAVDRNNTRVASLY
DSLHPSMLQVLKLIAVQARAVGLQVSLCGELAGDPMGALLLVGMGYRNLSMNGRSVARIKYLLRHIELSDAEVLAQRVLN
AQMTTEVRHQVAAFMERRGMGGLIRGGK

Sequences:

>Translated_748_residues
MLTRLREIVEKVAAAASLTDALDLLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKKPRGRAIALAFDEGIVGLVGR
RAEPINLADAQSHPSFKYIPQVKEDRFRSFLGVPIIHRRQLLGVLVVQQRELRQFDESEESFMVTLATQMAGILSQSQLN
AIFGQYRQTRIRALAASPGVAVAEGWQDSSQPSLDQVYKASTLDTASERERLTQALEEAGAEFRRFSKRFAASSQKESAA
IFDLYSHLLNDARLKRELFAEIDAGSVAEWAVKQVIEVFAAQFASLQDTYMRERASDLRALGQRLLFHLDDTTQGATQWP
ARFVLVADELTATLLAEMPQDRLVGVVVRDGAANSHAAILVRAMGVPTVMGADIQPALLSQRLLIVDGYRGELLVDPEPV
LVQEYQRLISEELELSRLAEDDVEQPAQLKSGERVQVMLNAGLSPEHEKLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMYPNKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAGTGNLGILLPMIT
SLEEIDEARRLIDRAGREVEEVLGYEIPKPKLGIMLEVPSMIFMIPHLAGRIEFISVGTNDLTQYLLAVDRNNTRVASLY
DSLHPSMLQVLKLIAVQARAVGLQVSLCGELAGDPMGALLLVGMGYRNLSMNGRSVARIKYLLRHIELSDAEVLAQRVLN
AQMTTEVRHQVAAFMERRGMGGLIRGGK
>Mature_748_residues
MLTRLREIVEKVAAAASLTDALDLLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKKPRGRAIALAFDEGIVGLVGR
RAEPINLADAQSHPSFKYIPQVKEDRFRSFLGVPIIHRRQLLGVLVVQQRELRQFDESEESFMVTLATQMAGILSQSQLN
AIFGQYRQTRIRALAASPGVAVAEGWQDSSQPSLDQVYKASTLDTASERERLTQALEEAGAEFRRFSKRFAASSQKESAA
IFDLYSHLLNDARLKRELFAEIDAGSVAEWAVKQVIEVFAAQFASLQDTYMRERASDLRALGQRLLFHLDDTTQGATQWP
ARFVLVADELTATLLAEMPQDRLVGVVVRDGAANSHAAILVRAMGVPTVMGADIQPALLSQRLLIVDGYRGELLVDPEPV
LVQEYQRLISEELELSRLAEDDVEQPAQLKSGERVQVMLNAGLSPEHEKLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMYPNKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAGTGNLGILLPMIT
SLEEIDEARRLIDRAGREVEEVLGYEIPKPKLGIMLEVPSMIFMIPHLAGRIEFISVGTNDLTQYLLAVDRNNTRVASLY
DSLHPSMLQVLKLIAVQARAVGLQVSLCGELAGDPMGALLLVGMGYRNLSMNGRSVARIKYLLRHIELSDAEVLAQRVLN
AQMTTEVRHQVAAFMERRGMGGLIRGGK

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=747, Percent_Identity=80.8567603748327, Blast_Score=1270, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=578, Percent_Identity=34.9480968858132, Blast_Score=305, Evalue=7e-84,
Organism=Escherichia coli, GI48994992, Length=512, Percent_Identity=32.6171875, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788726, Length=516, Percent_Identity=32.3643410852713, Blast_Score=233, Evalue=5e-62,
Organism=Escherichia coli, GI1787994, Length=404, Percent_Identity=25.4950495049505, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI226510935, Length=207, Percent_Identity=27.536231884058, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83068; Mature: 83068

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVAAAASLTDALDLLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEEEEECCCCEEEEEEECCCCCC
PRGRAIALAFDEGIVGLVGRRAEPINLADAQSHPSFKYIPQVKEDRFRSFLGVPIIHRRQ
CCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQFDESEESFMVTLATQMAGILSQSQLNAIFGQYRQTRIRALAASPGV
HHHHHHHHHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVAEGWQDSSQPSLDQVYKASTLDTASERERLTQALEEAGAEFRRFSKRFAASSQKESAA
EEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IFDLYSHLLNDARLKRELFAEIDAGSVAEWAVKQVIEVFAAQFASLQDTYMRERASDLRA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDTTQGATQWPARFVLVADELTATLLAEMPQDRLVGVVVRDGAANSHAAIL
HHHHHEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEE
VRAMGVPTVMGADIQPALLSQRLLIVDGYRGELLVDPEPVLVQEYQRLISEELELSRLAE
EEECCCCEEECCCCCHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVEQPAQLKSGERVQVMLNAGLSPEHEKLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
HHHCCCHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEECCCCHHHHCCCCCHHHHH
AQYQGMLQMYPNKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAGTGNLGILLPMITSLEEIDEARRLIDRAGREVEEVLGYEIPKPKLGIMLEVPS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEEECHH
MIFMIPHLAGRIEFISVGTNDLTQYLLAVDRNNTRVASLYDSLHPSMLQVLKLIAVQARA
HHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
VGLQVSLCGELAGDPMGALLLVGMGYRNLSMNGRSVARIKYLLRHIELSDAEVLAQRVLN
HCEEEEEHHHHCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
AQMTTEVRHQVAAFMERRGMGGLIRGGK
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTRLREIVEKVAAAASLTDALDLLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEEEEECCCCEEEEEEECCCCCC
PRGRAIALAFDEGIVGLVGRRAEPINLADAQSHPSFKYIPQVKEDRFRSFLGVPIIHRRQ
CCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQFDESEESFMVTLATQMAGILSQSQLNAIFGQYRQTRIRALAASPGV
HHHHHHHHHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVAEGWQDSSQPSLDQVYKASTLDTASERERLTQALEEAGAEFRRFSKRFAASSQKESAA
EEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IFDLYSHLLNDARLKRELFAEIDAGSVAEWAVKQVIEVFAAQFASLQDTYMRERASDLRA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDTTQGATQWPARFVLVADELTATLLAEMPQDRLVGVVVRDGAANSHAAIL
HHHHHEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEE
VRAMGVPTVMGADIQPALLSQRLLIVDGYRGELLVDPEPVLVQEYQRLISEELELSRLAE
EEECCCCEEECCCCCHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVEQPAQLKSGERVQVMLNAGLSPEHEKLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
HHHCCCHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEECCCCHHHHCCCCCHHHHH
AQYQGMLQMYPNKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAGTGNLGILLPMITSLEEIDEARRLIDRAGREVEEVLGYEIPKPKLGIMLEVPS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEEECHH
MIFMIPHLAGRIEFISVGTNDLTQYLLAVDRNNTRVASLYDSLHPSMLQVLKLIAVQARA
HHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
VGLQVSLCGELAGDPMGALLLVGMGYRNLSMNGRSVARIKYLLRHIELSDAEVLAQRVLN
HCEEEEEHHHHCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
AQMTTEVRHQVAAFMERRGMGGLIRGGK
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]