Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 157372056

GI number: 157372056

Start: 4228016

End: 4228810

Strand: Reverse

Name: thyA [H]

Synonym: Spro_3821

Alternate gene names: 157372056

Gene position: 4228810-4228016 (Counterclockwise)

Preceding gene: 157372057

Following gene: 157372055

Centisome position: 77.61

GC content: 54.21

Gene sequence:

>795_bases
ATGAAACAGTATCTGGATTTGATGAACAAAGTGCTCGCCGAGGGCACCCCTAAAGCCGACCGTACCGGCACTGGCACCTT
GTCGATATTCGGCCACCAGATGCGTTTCAATTTGCAGGAAGGGTTTCCGCTGGTCACTACCAAAAAGTGTCACCTGCGTT
CAATCATCCATGAGCTGCTGTGGTTCCTGAACGGTGACACCAACGTGGCTTACCTGAAGGAAAACAAAGTCAGCATCTGG
GACGAGTGGGCCGATGAAAACGGTAACCTTGGCCCGGTGTACGGCAAGCAGTGGCGTGCCTGGGGGACGGCGGACGGTCG
TCAGATCGATCAATTGACCACCGTGCTGAACCAGTTGAAACAGGACCCGGATTCACGCCGTATCATTGTTTCAGCCTGGA
ACGTGGGGGAGTTGGATCAGATGGCGTTGGCACCGTGCCACGCGTTCTTCCAGTTCTACGTGGCGGATGGCAAATTGTCT
TGCCAACTGTACCAGCGTTCCTGCGATGTGTTCCTCGGTTTGCCGTTCAACATTGCCAGCTACGCGCTATTGGTACATAT
GATGGCGCAGCAATGTGACCTGGAAGTGGGTGATTTCGTCTGGACCGGCGGCGACACTCATTTGTACAGCAACCATATGG
AACAAACCCAGCTGCAGTTGACGCGTGAGCCGCGTGCCTTGCCGAAATTGGTGATCAAGCGCAAGCCGGCATCGCTGTTT
GATTACCGTTTCGAAGACTTTGAAATCGAAGGTTACGATCCGCACCCAGCCATTAAGGCACCGGTTGCCATCTGA

Upstream 100 bases:

>100_bases
ATGGGGCAGATCCTGTCCGTCCCTATGGTGGTGGCCGGTATTATTATGATGATTTGGGCGTATCGTCGCCGCCCGCAGCA
ACAACTGTCGTGAGGAACAA

Downstream 100 bases:

>100_bases
TTTTTACCCGATGATTGATCCCCAGGGGCGCACAGGTTGCGCCCTTTTTTATTTCCGCCGATCCGCCTTGGTTGTTTCTG
CGTTCCTCATCGCATTTTAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLMNKVLAEGTPKADRTGTGTLSIFGHQMRFNLQEGFPLVTTKKCHLRSIIHELLWFLNGDTNVAYLKENKVSIW
DEWADENGNLGPVYGKQWRAWGTADGRQIDQLTTVLNQLKQDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMEQTQLQLTREPRALPKLVIKRKPASLF
DYRFEDFEIEGYDPHPAIKAPVAI

Sequences:

>Translated_264_residues
MKQYLDLMNKVLAEGTPKADRTGTGTLSIFGHQMRFNLQEGFPLVTTKKCHLRSIIHELLWFLNGDTNVAYLKENKVSIW
DEWADENGNLGPVYGKQWRAWGTADGRQIDQLTTVLNQLKQDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMEQTQLQLTREPRALPKLVIKRKPASLF
DYRFEDFEIEGYDPHPAIKAPVAI
>Mature_264_residues
MKQYLDLMNKVLAEGTPKADRTGTGTLSIFGHQMRFNLQEGFPLVTTKKCHLRSIIHELLWFLNGDTNVAYLKENKVSIW
DEWADENGNLGPVYGKQWRAWGTADGRQIDQLTTVLNQLKQDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMEQTQLQLTREPRALPKLVIKRKPASLF
DYRFEDFEIEGYDPHPAIKAPVAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=49.645390070922, Blast_Score=279, Evalue=2e-75,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=89.7727272727273, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.643109540636, Blast_Score=263, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=280, Percent_Identity=42.1428571428571, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=50, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30156; Mature: 30156

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLMNKVLAEGTPKADRTGTGTLSIFGHQMRFNLQEGFPLVTTKKCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLNGDTNVAYLKENKVSIWDEWADENGNLGPVYGKQWRAWGTADGRQIDQLTTVLNQLK
HHCCCCCCEEEEECCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCEEEECCHHHHH
YALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMEQTQLQLTREPRALPKLVIKRKPASLF
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
DYRFEDFEIEGYDPHPAIKAPVAI
CCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLMNKVLAEGTPKADRTGTGTLSIFGHQMRFNLQEGFPLVTTKKCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLNGDTNVAYLKENKVSIWDEWADENGNLGPVYGKQWRAWGTADGRQIDQLTTVLNQLK
HHCCCCCCEEEEECCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCEEEECCHHHHH
YALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMEQTQLQLTREPRALPKLVIKRKPASLF
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
DYRFEDFEIEGYDPHPAIKAPVAI
CCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA