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The map label for this gene is mltB [H]

Identifier: 157371636

GI number: 157371636

Start: 3757904

End: 3758989

Strand: Reverse

Name: mltB [H]

Synonym: Spro_3399

Alternate gene names: 157371636

Gene position: 3758989-3757904 (Counterclockwise)

Preceding gene: 157371638

Following gene: 157371634

Centisome position: 68.99

GC content: 58.2

Gene sequence:

>1086_bases
ATGCGTCATTTAGTTGTTCTTCTCCCTCTGCTTACCTTACTCACTGCCTGTAGCAGCGGGCCGAAGTCGACCCCAGCCAC
GCCTCAGGACAACACCGTGAAAGGCGGTTTTCTGCTGACACCAGCGCATTCCGGTCAGCCGTTGGGGGGCGATTTTGCCA
ACAATCCGAACACCGCCCGTTTTATCGATAAAATGGTGCAGGAACACGGTTTCGATCGGCAGCAGTTGCATGACGTGCTG
GCCCAGGCCAGGCGGTTAGACTCTGTACTGCGGCTGATGGATCGTCAGGCGCCGACGGCAACGACCCAGGCACCGTCCGG
CCCTAATGGTTCCTGGCTGCGTTACCGCAATAAATTTATTACGCCGGATAACGTGCAAAACGGCGTGCAGTTCTGGAATC
AGTATCAGGACGCGCTGCAGCGTGCTTATCAGACCTATGGTGTGCCACCAGAGATCATTGTCGGCATTATCGGCGTTGAA
ACCCGCTGGGGCCGCGTGATGGGTAAAACCCGCATTATCGATGCGCTGTCTACCCTGGCGTTCGACTATCCGCGCCGTGC
CGATTACTTTGCCGGCGAGCTGGAAACCTTCCTGTTGATGTCACGTACCGAAGGGGATGATCCGCTGGAGCTACGCGGCT
CCTTCGCAGGCGCCATGGGTTACGGTCAGTTTATGCCGTCCTCGTTCAAGAGTTACGCGGTTGATTTCAACGGTGACGGC
CACGTTAACCTGTGGGATCCGGTGGATGCCATCGGCAGCGTCGCCAACTACTTTAAAGCGCACGGCTGGACCAAGGGTGA
ACCTGTGGCCGTGCCGGCCAGCGGCCAGGCACCGGGGCTGGAAAATGGCTTTAAAACCCGCTATTCGGTGGCCAGTTTGT
CGGAAGCTGGGCTGACGCCGCAAGGGTCGCTTGGTGGCAATCAGGAAGCCAGCCTGCTGCGCCTGGATATGGGGACCAGC
TACCAGTATTGGTACGGTTTACCCAACTTCTACACCATTACCCGCTACAACCACAGTACCCATTATGCGATGGCGGTGTG
GCAGTTGGGCGAAGCGGTGGGCCGTTCGGCACGCGGCGGATTCTGA

Upstream 100 bases:

>100_bases
TTGTGGCGGTGTGATCGGCGACATAATCCGGTTTGTTCGCACCTGACAATGGATTAACATAACCCACACTTATCACTGGC
CTGGCTTATATGGATATCCG

Downstream 100 bases:

>100_bases
TGTCATTTCATTAATGGAAAAGGCCGCGGAAGCGGCCTTTTTTACATCGGTAGCGATTAGAAATCGTAACGCAGACGCAC
CAGCGTCATATCGCCCAGGT

Product: murein hydrolase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRHLVVLLPLLTLLTACSSGPKSTPATPQDNTVKGGFLLTPAHSGQPLGGDFANNPNTARFIDKMVQEHGFDRQQLHDVL
AQARRLDSVLRLMDRQAPTATTQAPSGPNGSWLRYRNKFITPDNVQNGVQFWNQYQDALQRAYQTYGVPPEIIVGIIGVE
TRWGRVMGKTRIIDALSTLAFDYPRRADYFAGELETFLLMSRTEGDDPLELRGSFAGAMGYGQFMPSSFKSYAVDFNGDG
HVNLWDPVDAIGSVANYFKAHGWTKGEPVAVPASGQAPGLENGFKTRYSVASLSEAGLTPQGSLGGNQEASLLRLDMGTS
YQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRSARGGF

Sequences:

>Translated_361_residues
MRHLVVLLPLLTLLTACSSGPKSTPATPQDNTVKGGFLLTPAHSGQPLGGDFANNPNTARFIDKMVQEHGFDRQQLHDVL
AQARRLDSVLRLMDRQAPTATTQAPSGPNGSWLRYRNKFITPDNVQNGVQFWNQYQDALQRAYQTYGVPPEIIVGIIGVE
TRWGRVMGKTRIIDALSTLAFDYPRRADYFAGELETFLLMSRTEGDDPLELRGSFAGAMGYGQFMPSSFKSYAVDFNGDG
HVNLWDPVDAIGSVANYFKAHGWTKGEPVAVPASGQAPGLENGFKTRYSVASLSEAGLTPQGSLGGNQEASLLRLDMGTS
YQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRSARGGF
>Mature_361_residues
MRHLVVLLPLLTLLTACSSGPKSTPATPQDNTVKGGFLLTPAHSGQPLGGDFANNPNTARFIDKMVQEHGFDRQQLHDVL
AQARRLDSVLRLMDRQAPTATTQAPSGPNGSWLRYRNKFITPDNVQNGVQFWNQYQDALQRAYQTYGVPPEIIVGIIGVE
TRWGRVMGKTRIIDALSTLAFDYPRRADYFAGELETFLLMSRTEGDDPLELRGSFAGAMGYGQFMPSSFKSYAVDFNGDG
HVNLWDPVDAIGSVANYFKAHGWTKGEPVAVPASGQAPGLENGFKTRYSVASLSEAGLTPQGSLGGNQEASLLRLDMGTS
YQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRSARGGF

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=353, Percent_Identity=76.4872521246459, Blast_Score=573, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 39656; Mature: 39656

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLVVLLPLLTLLTACSSGPKSTPATPQDNTVKGGFLLTPAHSGQPLGGDFANNPNTAR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHH
FIDKMVQEHGFDRQQLHDVLAQARRLDSVLRLMDRQAPTATTQAPSGPNGSWLRYRNKFI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCC
TPDNVQNGVQFWNQYQDALQRAYQTYGVPPEIIVGIIGVETRWGRVMGKTRIIDALSTLA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHH
FDYPRRADYFAGELETFLLMSRTEGDDPLELRGSFAGAMGYGQFMPSSFKSYAVDFNGDG
HCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHEEEEECCCC
HVNLWDPVDAIGSVANYFKAHGWTKGEPVAVPASGQAPGLENGFKTRYSVASLSEAGLTP
CEEECCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QGSLGGNQEASLLRLDMGTSYQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRSARGG
CCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
F
C
>Mature Secondary Structure
MRHLVVLLPLLTLLTACSSGPKSTPATPQDNTVKGGFLLTPAHSGQPLGGDFANNPNTAR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHH
FIDKMVQEHGFDRQQLHDVLAQARRLDSVLRLMDRQAPTATTQAPSGPNGSWLRYRNKFI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCC
TPDNVQNGVQFWNQYQDALQRAYQTYGVPPEIIVGIIGVETRWGRVMGKTRIIDALSTLA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHH
FDYPRRADYFAGELETFLLMSRTEGDDPLELRGSFAGAMGYGQFMPSSFKSYAVDFNGDG
HCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHEEEEECCCC
HVNLWDPVDAIGSVANYFKAHGWTKGEPVAVPASGQAPGLENGFKTRYSVASLSEAGLTP
CEEECCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QGSLGGNQEASLLRLDMGTSYQYWYGLPNFYTITRYNHSTHYAMAVWQLGEAVGRSARGG
CCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]