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The map label for this gene is folC [H]

Identifier: 157371568

GI number: 157371568

Start: 3686161

End: 3687423

Strand: Reverse

Name: folC [H]

Synonym: Spro_3331

Alternate gene names: 157371568

Gene position: 3687423-3686161 (Counterclockwise)

Preceding gene: 157371569

Following gene: 157371567

Centisome position: 67.67

GC content: 60.73

Gene sequence:

>1263_bases
ATGCAAAACCACCAAATTCCCCAAGCCACGTCGCCATTGAGCTCGTGGCTTTTCTATCTGGAACGTCTGCACAGCCAGGC
GATCGAACTCGGCCTGGAACGCGTACAGCGCGTTGCGGCGCATCTTGATTTACTCACTCCTGCACCGACGGTATTTACCG
TAGCCGGGACCAATGGCAAAGGCACCACCTGTCGCACCCTGGAAACCATCCTGATGGCGGCCGGTTTGCGGGTTGGCGTC
TACAGCTCGCCGCACCTGGTGCGTTATACCGAGCGGGTGCGCATTCAGGGCGATGAACTGAGTGAGGCCGAACACTGCCG
CTCGTTCGCCGCCATTGAAGCGGGCCGGGGCGAAACTTCACTGACCTACTTCGAATTTGGCACCCTGTCGGCGCTGCAAC
TGTTCAAACAGGCTAAGCTTGACGTGGTGATCCTGGAAGTCGGGCTGGGTGGCCGCCTGGATGCGACCAACATCGTCGAT
CCGAGCGTGGCAGTGGTGACCAGCATTGCGCTGGATCACACCGACTGGCTGGGCAACGATCGCGAAAGCATTGGTCGTGA
AAAGGCCGGGATCTTCCGCAGCGGCAAACCGGCGGTGGTGGGGGAACCGGATATGCCGGCCACCATTGGGCAGGTGGCGG
AAACGCTGGGTGCCCAACTGTATCGCCGCGGCGATGCCTGGAGCTTCAGCGAACAGGGCGAACGCTGGCAGTGGCAAGGC
GGCGGTACCTTGCTGACCGATTTGCCGATGCCCAACGTACCGTTAGCCAATGCGGCGACGGCACTGGCTGCGCTGCATTT
TTCTGCGTTGGAAATTGATGAGCGGGCAATCTTCACCGGCTTGCAGCAGGCGACATTACCTGGACGTTTCCAGATTGTGC
GACGCGATCCGATGCTGATCCTCGACGTGGCGCATAATCCCCACGCGGCAGGTTACCTGGCCGGGCGTCTGGCGACCTTA
CCGCGCAATGGCGGCAAGGTGCGAGCGGTGGTGGGGATGTTGTCGGATAAAGACATCGCCGGCACCCTGGCCTGTTTGAG
TGAGCAGGTCGATGAGTGGTATTGCGCGCCGCTGGACGGGCCGCGCGGGGCCAGCGTCGAACTGTTGGCGCAATATTTGA
CGGAACCGCGGCAGTTTGCCGATGTCGAAACTGCCTGGCGTCAGGCTATGCAGGACGCAGATATTCAGGATATTGTGATC
GTCTGTGGATCGTTCCACACCGTTGCACAGGTTATGGCGGCGTTAGACGAGCGGGGAGTGTGA

Upstream 100 bases:

>100_bases
TTTGTGGCTAAATGACGGCTGATAACGGAGAGCATCGCCTTTACCGCGGTGAGGGTGGTGCCCTGATGGATTAATGAACC
CAAGTTCAGTGACGGGACTC

Downstream 100 bases:

>100_bases
GTGGCAAGTAAATTTCAAAACCGATTGGTCGGTACCGTGATTTTGGTCGCGCTGGGGGTGATTATCCTGCCAGGGCTGCT
GGACGGTAAAAAGAAACATT

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQNHQIPQATSPLSSWLFYLERLHSQAIELGLERVQRVAAHLDLLTPAPTVFTVAGTNGKGTTCRTLETILMAAGLRVGV
YSSPHLVRYTERVRIQGDELSEAEHCRSFAAIEAGRGETSLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVD
PSVAVVTSIALDHTDWLGNDRESIGREKAGIFRSGKPAVVGEPDMPATIGQVAETLGAQLYRRGDAWSFSEQGERWQWQG
GGTLLTDLPMPNVPLANAATALAALHFSALEIDERAIFTGLQQATLPGRFQIVRRDPMLILDVAHNPHAAGYLAGRLATL
PRNGGKVRAVVGMLSDKDIAGTLACLSEQVDEWYCAPLDGPRGASVELLAQYLTEPRQFADVETAWRQAMQDADIQDIVI
VCGSFHTVAQVMAALDERGV

Sequences:

>Translated_420_residues
MQNHQIPQATSPLSSWLFYLERLHSQAIELGLERVQRVAAHLDLLTPAPTVFTVAGTNGKGTTCRTLETILMAAGLRVGV
YSSPHLVRYTERVRIQGDELSEAEHCRSFAAIEAGRGETSLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVD
PSVAVVTSIALDHTDWLGNDRESIGREKAGIFRSGKPAVVGEPDMPATIGQVAETLGAQLYRRGDAWSFSEQGERWQWQG
GGTLLTDLPMPNVPLANAATALAALHFSALEIDERAIFTGLQQATLPGRFQIVRRDPMLILDVAHNPHAAGYLAGRLATL
PRNGGKVRAVVGMLSDKDIAGTLACLSEQVDEWYCAPLDGPRGASVELLAQYLTEPRQFADVETAWRQAMQDADIQDIVI
VCGSFHTVAQVMAALDERGV
>Mature_420_residues
MQNHQIPQATSPLSSWLFYLERLHSQAIELGLERVQRVAAHLDLLTPAPTVFTVAGTNGKGTTCRTLETILMAAGLRVGV
YSSPHLVRYTERVRIQGDELSEAEHCRSFAAIEAGRGETSLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVD
PSVAVVTSIALDHTDWLGNDRESIGREKAGIFRSGKPAVVGEPDMPATIGQVAETLGAQLYRRGDAWSFSEQGERWQWQG
GGTLLTDLPMPNVPLANAATALAALHFSALEIDERAIFTGLQQATLPGRFQIVRRDPMLILDVAHNPHAAGYLAGRLATL
PRNGGKVRAVVGMLSDKDIAGTLACLSEQVDEWYCAPLDGPRGASVELLAQYLTEPRQFADVETAWRQAMQDADIQDIVI
VCGSFHTVAQVMAALDERGV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=374, Percent_Identity=32.0855614973262, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI66932990, Length=374, Percent_Identity=32.0855614973262, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1788654, Length=414, Percent_Identity=67.6328502415459, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71984923, Length=321, Percent_Identity=31.1526479750779, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17553148, Length=321, Percent_Identity=31.7757009345794, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17553150, Length=321, Percent_Identity=31.7757009345794, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=313, Percent_Identity=30.0319488817891, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324815, Length=175, Percent_Identity=35.4285714285714, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24641571, Length=177, Percent_Identity=37.2881355932203, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24581568, Length=203, Percent_Identity=34.4827586206897, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45535; Mature: 45535

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNHQIPQATSPLSSWLFYLERLHSQAIELGLERVQRVAAHLDLLTPAPTVFTVAGTNGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GTTCRTLETILMAAGLRVGVYSSPHLVRYTERVRIQGDELSEAEHCRSFAAIEAGRGETS
CCHHHHHHHHHHHCCCEEEECCCCCCEEEEHHEEECCCCCCHHHHHHHHHHHCCCCCCCE
LTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVDPSVAVVTSIALDHTDWLGND
EEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCC
RESIGREKAGIFRSGKPAVVGEPDMPATIGQVAETLGAQLYRRGDAWSFSEQGERWQWQG
HHHHCHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
GGTLLTDLPMPNVPLANAATALAALHFSALEIDERAIFTGLQQATLPGRFQIVRRDPMLI
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEE
LDVAHNPHAAGYLAGRLATLPRNGGKVRAVVGMLSDKDIAGTLACLSEQVDEWYCAPLDG
EEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCEEECCCCC
PRGASVELLAQYLTEPRQFADVETAWRQAMQDADIQDIVIVCGSFHTVAQVMAALDERGV
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNHQIPQATSPLSSWLFYLERLHSQAIELGLERVQRVAAHLDLLTPAPTVFTVAGTNGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GTTCRTLETILMAAGLRVGVYSSPHLVRYTERVRIQGDELSEAEHCRSFAAIEAGRGETS
CCHHHHHHHHHHHCCCEEEECCCCCCEEEEHHEEECCCCCCHHHHHHHHHHHCCCCCCCE
LTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVDPSVAVVTSIALDHTDWLGND
EEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCC
RESIGREKAGIFRSGKPAVVGEPDMPATIGQVAETLGAQLYRRGDAWSFSEQGERWQWQG
HHHHCHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
GGTLLTDLPMPNVPLANAATALAALHFSALEIDERAIFTGLQQATLPGRFQIVRRDPMLI
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEE
LDVAHNPHAAGYLAGRLATLPRNGGKVRAVVGMLSDKDIAGTLACLSEQVDEWYCAPLDG
EEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCEEECCCCC
PRGASVELLAQYLTEPRQFADVETAWRQAMQDADIQDIVIVCGSFHTVAQVMAALDERGV
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]