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The map label for this gene is puuA [H]

Identifier: 157371453

GI number: 157371453

Start: 3542601

End: 3544019

Strand: Reverse

Name: puuA [H]

Synonym: Spro_3216

Alternate gene names: 157371453

Gene position: 3544019-3542601 (Counterclockwise)

Preceding gene: 157371458

Following gene: 157371452

Centisome position: 65.04

GC content: 56.52

Gene sequence:

>1419_bases
ATGCAAACCAATATCGCAGCAGTTGAAAATTTTGCACAGCATAATGAAGAGAGGCGAAGTAGCGCGTTCCAACGTGAGGT
TGCTCATTATTTGGAACGCCACCCCTCAACGCAGTATATCGATATCCTTCTCACTGATCTGAATGGTTCCTTCCGCGGTA
AACGCATCCCTGTCTCCGGACTGAAAAAATTGGAAAAAGGCTGTTACTTCCCCGCCTCGGTATTCGCTATGGATATTTTG
GGCAATGTGGTGGAAGAGGCGGGCCTGGGCCAGGAACTGGGTGAACCGGACCGTGTATGCATGCCGGTGTCGGGTTCACT
GACTCCCTCAGCGGCGGACCCGGATAATATCGGTCAGGTATTACTGACCATGCTGGATGAAGATGGCACTCCCTTTGACG
TTGAACCCCGTAATGTCCTGAATCGAGTGTGGCAGGCGTTGCGCCAGCGCGGCATGTTCCCGGTGGTAGCGGTAGAGTTG
GAGTTCTATCTCATTGACAGGCAGCGAGACTCTGAGGGGTATTTGCAGCCCCCCTGTGCGCCCGGTACCCAGGAGCGCAA
CATGCAGAGCCAGGTTTACTCGCTGGATAACCTGAATCATTTTGCCGAAGTGCTTAACGACATCGACACGCTGGCGCAGA
TGCAGGGCTTACCGGCAGACGGTGCGGTGGCGGAGGCGTCCCCCGGCCAGTTCGAGGTGAATCTGCAGCATACCGATAAT
GTGTTGCTGGCCTGTGACCATGCGCTGGGGTTAAAGCGACTGGTGCGGATGGTGGCGGAAAATCATCATTTGCACGCCAC
CTTTATGGCCAAACCCTATGAAGAATACGCCGGTAGTGGCATGCACGTGCATATCAGCATGCTGGACGGCAACGGCAACA
ACCTGTTCGCGCAAGCGGACGGCGAAGACTCGACGCTGCTGCACCGCGCGCTGGCCGGGATGATCACCCTAATGCCGGCC
TCCATGGCGCTGCTGGCGCCCAATGTGAACGCTTTTCGCCGTTTCCAGCCGGGTATGTATGTGCCTACCCAGGCATCATG
GGGGCACAACAATCGCACCGTTGCACTGCGCATCCCGTGTGGCGAACCGGAAAATCATCGGGTGGAATACCGCGTTGCGG
GTGCGGATGCCAACCCTTATCTGGTGGTGGCGGCGATACTGTCAGGCATTCTTTACGGCCTGGACAACGAATTGCCCTTG
CCTGAGCCGGTGACCGGCAACGGGCTGGAGCAGGAAGGGCTGCCGTTCCCTATCCGTCAAAGCGACGCCCTGTACGAGTT
TGAGCACCAGCATGAACTGACGCAGTATCTGGGCGAACGTTTTGCCCACGTTTACCACGCCTGCAAGCTGGACGAACTGA
TGCAGTTTGAGCGACTGGTGACGGAAACCGAGATTGACTGGATGTTGAAAAATGCCTGA

Upstream 100 bases:

>100_bases
GCGCCAGTTCTGGCGCAGTCTACAGAGAAATCAGACCATGTGTTGAACCTATCGGCAGGTGAACGCGAACGCGTTCTCAG
AGCATAATGGCGGGTGATGT

Downstream 100 bases:

>100_bases
AGCGGCGTAAAATGTGCGTCATTAACTTGATAGGCGATAGAACGATAGCGGATTAATTCACCTGTAAGGGTTGTTTTCCG
AACGATCGCGAAAAGAATTT

Product: glutamine synthetase catalytic region

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQTNIAAVENFAQHNEERRSSAFQREVAHYLERHPSTQYIDILLTDLNGSFRGKRIPVSGLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQELGEPDRVCMPVSGSLTPSAADPDNIGQVLLTMLDEDGTPFDVEPRNVLNRVWQALRQRGMFPVVAVEL
EFYLIDRQRDSEGYLQPPCAPGTQERNMQSQVYSLDNLNHFAEVLNDIDTLAQMQGLPADGAVAEASPGQFEVNLQHTDN
VLLACDHALGLKRLVRMVAENHHLHATFMAKPYEEYAGSGMHVHISMLDGNGNNLFAQADGEDSTLLHRALAGMITLMPA
SMALLAPNVNAFRRFQPGMYVPTQASWGHNNRTVALRIPCGEPENHRVEYRVAGADANPYLVVAAILSGILYGLDNELPL
PEPVTGNGLEQEGLPFPIRQSDALYEFEHQHELTQYLGERFAHVYHACKLDELMQFERLVTETEIDWMLKNA

Sequences:

>Translated_472_residues
MQTNIAAVENFAQHNEERRSSAFQREVAHYLERHPSTQYIDILLTDLNGSFRGKRIPVSGLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQELGEPDRVCMPVSGSLTPSAADPDNIGQVLLTMLDEDGTPFDVEPRNVLNRVWQALRQRGMFPVVAVEL
EFYLIDRQRDSEGYLQPPCAPGTQERNMQSQVYSLDNLNHFAEVLNDIDTLAQMQGLPADGAVAEASPGQFEVNLQHTDN
VLLACDHALGLKRLVRMVAENHHLHATFMAKPYEEYAGSGMHVHISMLDGNGNNLFAQADGEDSTLLHRALAGMITLMPA
SMALLAPNVNAFRRFQPGMYVPTQASWGHNNRTVALRIPCGEPENHRVEYRVAGADANPYLVVAAILSGILYGLDNELPL
PEPVTGNGLEQEGLPFPIRQSDALYEFEHQHELTQYLGERFAHVYHACKLDELMQFERLVTETEIDWMLKNA
>Mature_472_residues
MQTNIAAVENFAQHNEERRSSAFQREVAHYLERHPSTQYIDILLTDLNGSFRGKRIPVSGLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQELGEPDRVCMPVSGSLTPSAADPDNIGQVLLTMLDEDGTPFDVEPRNVLNRVWQALRQRGMFPVVAVEL
EFYLIDRQRDSEGYLQPPCAPGTQERNMQSQVYSLDNLNHFAEVLNDIDTLAQMQGLPADGAVAEASPGQFEVNLQHTDN
VLLACDHALGLKRLVRMVAENHHLHATFMAKPYEEYAGSGMHVHISMLDGNGNNLFAQADGEDSTLLHRALAGMITLMPA
SMALLAPNVNAFRRFQPGMYVPTQASWGHNNRTVALRIPCGEPENHRVEYRVAGADANPYLVVAAILSGILYGLDNELPL
PEPVTGNGLEQEGLPFPIRQSDALYEFEHQHELTQYLGERFAHVYHACKLDELMQFERLVTETEIDWMLKNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=433, Percent_Identity=25.1732101616628, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=80.2966101694915, Blast_Score=802, Evalue=0.0,
Organism=Escherichia coli, GI1790301, Length=391, Percent_Identity=27.3657289002558, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52573; Mature: 52573

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNIAAVENFAQHNEERRSSAFQREVAHYLERHPSTQYIDILLTDLNGSFRGKRIPVSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHH
LKKLEKGCYFPASVFAMDILGNVVEEAGLGQELGEPDRVCMPVSGSLTPSAADPDNIGQV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHH
LLTMLDEDGTPFDVEPRNVLNRVWQALRQRGMFPVVAVELEFYLIDRQRDSEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCCC
PGTQERNMQSQVYSLDNLNHFAEVLNDIDTLAQMQGLPADGAVAEASPGQFEVNLQHTDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCC
VLLACDHALGLKRLVRMVAENHHLHATFMAKPYEEYAGSGMHVHISMLDGNGNNLFAQAD
EEEEECHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEEEEECCCCEEEEECC
GEDSTLLHRALAGMITLMPASMALLAPNVNAFRRFQPGMYVPTQASWGHNNRTVALRIPC
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GEPENHRVEYRVAGADANPYLVVAAILSGILYGLDNELPLPEPVTGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDALYEFEHQHELTQYLGERFAHVYHACKLDELMQFERLVTETEIDWMLKNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCC
>Mature Secondary Structure
MQTNIAAVENFAQHNEERRSSAFQREVAHYLERHPSTQYIDILLTDLNGSFRGKRIPVSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHH
LKKLEKGCYFPASVFAMDILGNVVEEAGLGQELGEPDRVCMPVSGSLTPSAADPDNIGQV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHH
LLTMLDEDGTPFDVEPRNVLNRVWQALRQRGMFPVVAVELEFYLIDRQRDSEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCCC
PGTQERNMQSQVYSLDNLNHFAEVLNDIDTLAQMQGLPADGAVAEASPGQFEVNLQHTDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCC
VLLACDHALGLKRLVRMVAENHHLHATFMAKPYEEYAGSGMHVHISMLDGNGNNLFAQAD
EEEEECHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEEEEECCCCEEEEECC
GEDSTLLHRALAGMITLMPASMALLAPNVNAFRRFQPGMYVPTQASWGHNNRTVALRIPC
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GEPENHRVEYRVAGADANPYLVVAAILSGILYGLDNELPLPEPVTGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDALYEFEHQHELTQYLGERFAHVYHACKLDELMQFERLVTETEIDWMLKNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]