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The map label for this gene is citT [H]

Identifier: 157371404

GI number: 157371404

Start: 3483402

End: 3484865

Strand: Reverse

Name: citT [H]

Synonym: Spro_3165

Alternate gene names: 157371404

Gene position: 3484865-3483402 (Counterclockwise)

Preceding gene: 157371405

Following gene: 157371403

Centisome position: 63.96

GC content: 59.36

Gene sequence:

>1464_bases
ATGTCCGAAACCAAGGAAAAAGTCTGGAAAACGCTGGCACCGCTGCTGGTGTTGGCGATCCTGCTCTGGATACCCGCCCC
GGAAGGGATGCCGCCGCAAGCCTGGCGTTACTTTGCCATTTTTGTGGCAATGATTGTCGGCATGATCCTGGAGCCGATCC
CGGCGACGGCGATCAGTTTTATCGCCGTGACCCTCAGCGTGTTGAGCGCCAACTGGGTGCTGTTTGGTGCGCAGGAACTG
GCAGAACCGGGTTTTAAGGCCGGTAAAGAGGCCCTGAAATGGGGGCTGGCGGGGTTCTCCAGCACTACCGTGTGGCTGGT
GTTCGGTGCCTTTATCTTCGCGCTTGGCTACGAGGCTACCGGGCTGGGGCGGCGTATCGCGCTATTTATGGTGAAGTTTA
TGGGTAAGCGTACCCTGACGCTCGGCTATGCGGTGGTGATCATCGATATCCTGCTGGCGCCGTTTACCCCGTCCAATACC
GCGCGTACCGGCGGTACCGTGTTCCCGGTGGTGAAAAACCTGCCGCCGTTGTTCGACTCGTTCCCCAATGATCCTTCCTC
ACGCCGTATTGGCGGCTACCTGATGTGGATGATGGTAGTGGGGACCAGCATCAGCTCCTCAATGTTCGTCACCGGTGCCG
CGCCCAACGTGCTGGGGATCGAGTTCGTCAGCAAGATTGCCGGCGTGCATATCAGTTGGATGCAGTGGTTCCTGGCCTTC
CTGCCGGTTGGGCTGTTGCTGTTGATTATCGCGCCGTTGATATCCTATTACCTGTACAAACCGGGCGTGACCCACAGCAG
TGAAGTGGCGACCTGGGCCAATACCGCGCTGAACGAGATGGGCAAGCTGACGCGTCAGGAAATCAAGCTGATTGGCCTGG
TGCTGCTGAGCCTGTGCCTGTGGGTGTTTGGCGGCAAGGTGGTGAATGCCACCACGGTCTGCCTGCTGGCGGTTTCCCTG
ATGCTGGCGTTGAATGTGGTGTCGTGGAAGGCGATCACCCAATATTCCAGCGCCTGGAACACGCTGGTTAACCTGGCGAC
GCTGGTGGTGATGGCCAACGGCCTGACGCGCTCCGGCTTTATCGACTGGTTCGCCGAGACCATGAGCACCCATCTGGACG
GTTTCTCGCCGAATATGACGGTGGCGGCGCTGGTGCTGGTGTTCTACTTTGCCCACTATCTGTTCGCCAGCCTGTCGGCG
CACACCGCTACCATGCTGCCGGTGATCCTGGCGGTCGGCAAGGGGCTGCCGGGGGTGCCGATGGAGGAACTCTCGATGTT
ATTGGTGCTGTCGATCGGCATCATGGGCGTGCTGACGCCTTACGCCACTGGTCCGGGGGTGATCATCTACGGCTGCGGCT
ATGTGAAATCGAAGGATTACTGGCGCATGGGCGGCATTCTCGGCGTGGTGTATATCGCCGCGCTGCTGATGATCGGTTGG
CCAATCATGCGTTTGTGGCTGTAA

Upstream 100 bases:

>100_bases
GCAATCTCAGCCCCGGCGGCAGCGCCGACCTGTTAATCGTCACCTGGCTGCTGGCGCAGATAACGAACTAAAAACAATAA
CCCGTCTTCAGGAGAATTTT

Downstream 100 bases:

>100_bases
GTTTTTGAGTTAATGGCGGCCATCTGCGGATGGCCGCTGGGCAGACCTTTCCTTGTGATATCCATCACACACTTTCCCCG
TTAACCTCTCCATCATAGCG

Product: anion transporter

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSETKEKVWKTLAPLLVLAILLWIPAPEGMPPQAWRYFAIFVAMIVGMILEPIPATAISFIAVTLSVLSANWVLFGAQEL
AEPGFKAGKEALKWGLAGFSSTTVWLVFGAFIFALGYEATGLGRRIALFMVKFMGKRTLTLGYAVVIIDILLAPFTPSNT
ARTGGTVFPVVKNLPPLFDSFPNDPSSRRIGGYLMWMMVVGTSISSSMFVTGAAPNVLGIEFVSKIAGVHISWMQWFLAF
LPVGLLLLIIAPLISYYLYKPGVTHSSEVATWANTALNEMGKLTRQEIKLIGLVLLSLCLWVFGGKVVNATTVCLLAVSL
MLALNVVSWKAITQYSSAWNTLVNLATLVVMANGLTRSGFIDWFAETMSTHLDGFSPNMTVAALVLVFYFAHYLFASLSA
HTATMLPVILAVGKGLPGVPMEELSMLLVLSIGIMGVLTPYATGPGVIIYGCGYVKSKDYWRMGGILGVVYIAALLMIGW
PIMRLWL

Sequences:

>Translated_487_residues
MSETKEKVWKTLAPLLVLAILLWIPAPEGMPPQAWRYFAIFVAMIVGMILEPIPATAISFIAVTLSVLSANWVLFGAQEL
AEPGFKAGKEALKWGLAGFSSTTVWLVFGAFIFALGYEATGLGRRIALFMVKFMGKRTLTLGYAVVIIDILLAPFTPSNT
ARTGGTVFPVVKNLPPLFDSFPNDPSSRRIGGYLMWMMVVGTSISSSMFVTGAAPNVLGIEFVSKIAGVHISWMQWFLAF
LPVGLLLLIIAPLISYYLYKPGVTHSSEVATWANTALNEMGKLTRQEIKLIGLVLLSLCLWVFGGKVVNATTVCLLAVSL
MLALNVVSWKAITQYSSAWNTLVNLATLVVMANGLTRSGFIDWFAETMSTHLDGFSPNMTVAALVLVFYFAHYLFASLSA
HTATMLPVILAVGKGLPGVPMEELSMLLVLSIGIMGVLTPYATGPGVIIYGCGYVKSKDYWRMGGILGVVYIAALLMIGW
PIMRLWL
>Mature_486_residues
SETKEKVWKTLAPLLVLAILLWIPAPEGMPPQAWRYFAIFVAMIVGMILEPIPATAISFIAVTLSVLSANWVLFGAQELA
EPGFKAGKEALKWGLAGFSSTTVWLVFGAFIFALGYEATGLGRRIALFMVKFMGKRTLTLGYAVVIIDILLAPFTPSNTA
RTGGTVFPVVKNLPPLFDSFPNDPSSRRIGGYLMWMMVVGTSISSSMFVTGAAPNVLGIEFVSKIAGVHISWMQWFLAFL
PVGLLLLIIAPLISYYLYKPGVTHSSEVATWANTALNEMGKLTRQEIKLIGLVLLSLCLWVFGGKVVNATTVCLLAVSLM
LALNVVSWKAITQYSSAWNTLVNLATLVVMANGLTRSGFIDWFAETMSTHLDGFSPNMTVAALVLVFYFAHYLFASLSAH
TATMLPVILAVGKGLPGVPMEELSMLLVLSIGIMGVLTPYATGPGVIIYGCGYVKSKDYWRMGGILGVVYIAALLMIGWP
IMRLWL

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=486, Percent_Identity=80.4526748971193, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI1789444, Length=471, Percent_Identity=43.5244161358811, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786986, Length=470, Percent_Identity=34.2553191489362, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 52861; Mature: 52730

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKEKVWKTLAPLLVLAILLWIPAPEGMPPQAWRYFAIFVAMIVGMILEPIPATAISF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTLSVLSANWVLFGAQELAEPGFKAGKEALKWGLAGFSSTTVWLVFGAFIFALGYEAT
HHHHHHHHHHCHHEECHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
GLGRRIALFMVKFMGKRTLTLGYAVVIIDILLAPFTPSNTARTGGTVFPVVKNLPPLFDS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHC
FPNDPSSRRIGGYLMWMMVVGTSISSSMFVTGAAPNVLGIEFVSKIAGVHISWMQWFLAF
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LPVGLLLLIIAPLISYYLYKPGVTHSSEVATWANTALNEMGKLTRQEIKLIGLVLLSLCL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGGKVVNATTVCLLAVSLMLALNVVSWKAITQYSSAWNTLVNLATLVVMANGLTRSGF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFAETMSTHLDGFSPNMTVAALVLVFYFAHYLFASLSAHTATMLPVILAVGKGLPGVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEELSMLLVLSIGIMGVLTPYATGPGVIIYGCGYVKSKDYWRMGGILGVVYIAALLMIGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PIMRLWL
HHHHHCC
>Mature Secondary Structure 
SETKEKVWKTLAPLLVLAILLWIPAPEGMPPQAWRYFAIFVAMIVGMILEPIPATAISF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTLSVLSANWVLFGAQELAEPGFKAGKEALKWGLAGFSSTTVWLVFGAFIFALGYEAT
HHHHHHHHHHCHHEECHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
GLGRRIALFMVKFMGKRTLTLGYAVVIIDILLAPFTPSNTARTGGTVFPVVKNLPPLFDS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHC
FPNDPSSRRIGGYLMWMMVVGTSISSSMFVTGAAPNVLGIEFVSKIAGVHISWMQWFLAF
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LPVGLLLLIIAPLISYYLYKPGVTHSSEVATWANTALNEMGKLTRQEIKLIGLVLLSLCL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGGKVVNATTVCLLAVSLMLALNVVSWKAITQYSSAWNTLVNLATLVVMANGLTRSGF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFAETMSTHLDGFSPNMTVAALVLVFYFAHYLFASLSAHTATMLPVILAVGKGLPGVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEELSMLLVLSIGIMGVLTPYATGPGVIIYGCGYVKSKDYWRMGGILGVVYIAALLMIGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PIMRLWL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]