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The map label for this gene is nac [H]

Identifier: 157371368

GI number: 157371368

Start: 3445776

End: 3446699

Strand: Reverse

Name: nac [H]

Synonym: Spro_3129

Alternate gene names: 157371368

Gene position: 3446699-3445776 (Counterclockwise)

Preceding gene: 157371369

Following gene: 157371360

Centisome position: 63.26

GC content: 58.44

Gene sequence:

>924_bases
ATGAATTTCAGACGTCTTAAGTATTTCGTGAAAATCGTCGACATCGGCAGCCTGACCCAGGCGGCAGAAGTGCTGCATAT
CGCCCAACCGGCACTCAGCCAACAATTGGCGACACTGGAAGGAGAGCTGAAACAGCAGCTGCTGATCCGCACCAAACGCG
GGGTACAGCCTACTGAGGCCGGTAATATTCTTTATGCCCATGCCCAGACTATTTTGCGCCAGTGCGAACAGGCGCAAAGC
GCGGTCAATAGTGCCGGGCAGGCGATGAGCGGGCAGGTCTCTGTCGGGCTGGCCCCGGGCAGTCTGGCATCACAGTTGGC
GTTGCCGTTGTTGCAGGCGGTGCGCGATCAGCATCCGGGGATTTTGCTCAGTCTGAATGAGAATATTGGCTCGACGCTGG
CCGGCCAGGTCGCTAACCAAAACCTGGATATGGCGGTGATCTACGGCGCTAAAATGCCCGCAGGTTTGCAGGCCACGGCC
TTAATGCGCGAAGATCTCTATCTGGTGGCGACGCGTGCGGTGCCAAACCCTGGCAACAGCGTTGAGCTGCTGGACGTTGC
GCGGCTGAATTTATTTTTGCCGCGTGAGGGAGACGTGGTACGCAGTCAGGTGGACGAAGCCATGGCGATACGCAAGTTAA
CGGCCAACGTGATTGGTGAAATTGAATCGCCTGGCACCTTGAGTGCGGCTATCGCCAGTGGTTTGGGGGCCACCATTTTG
CCAGAGTCGGTGGCGCGCGCGATGATTGGCCCGGCCAAGGCATGGATGGCGCGTATCAATGCTCCGTCGCTGAGCGTGCC
GCTTTCGCTGTGCATTTCCGGCCAGCAATCCTTGTCGGCCCCGGCGCTGGTGGTGAAAGAGATCCTGCTGTCAATTGCCG
CCAGCCGCAGTCAGGAAAAACGCGTGCTGGCGTTGGTGCAATAA

Upstream 100 bases:

>100_bases
TTTTCCGTCGATTAGTTGACTCTTTCTCTTATTCCGTCTGTCTGGCCCGGATCTTGCTGTGGTTTATCTTAACCCAGCCA
AGTGGCATGACGGAGGCAGT

Downstream 100 bases:

>100_bases
ATCAGGTCGAACTCGATTAGCTGAGTTGGTAACGGCTGTCCGGCTGGGTAGCGGCGGGTAAATCCGTCTCGTCATTCATT
TCGCGCAGGTTGATCTCAAT

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQLATLEGELKQQLLIRTKRGVQPTEAGNILYAHAQTILRQCEQAQS
AVNSAGQAMSGQVSVGLAPGSLASQLALPLLQAVRDQHPGILLSLNENIGSTLAGQVANQNLDMAVIYGAKMPAGLQATA
LMREDLYLVATRAVPNPGNSVELLDVARLNLFLPREGDVVRSQVDEAMAIRKLTANVIGEIESPGTLSAAIASGLGATIL
PESVARAMIGPAKAWMARINAPSLSVPLSLCISGQQSLSAPALVVKEILLSIAASRSQEKRVLALVQ

Sequences:

>Translated_307_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQLATLEGELKQQLLIRTKRGVQPTEAGNILYAHAQTILRQCEQAQS
AVNSAGQAMSGQVSVGLAPGSLASQLALPLLQAVRDQHPGILLSLNENIGSTLAGQVANQNLDMAVIYGAKMPAGLQATA
LMREDLYLVATRAVPNPGNSVELLDVARLNLFLPREGDVVRSQVDEAMAIRKLTANVIGEIESPGTLSAAIASGLGATIL
PESVARAMIGPAKAWMARINAPSLSVPLSLCISGQQSLSAPALVVKEILLSIAASRSQEKRVLALVQ
>Mature_307_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQLATLEGELKQQLLIRTKRGVQPTEAGNILYAHAQTILRQCEQAQS
AVNSAGQAMSGQVSVGLAPGSLASQLALPLLQAVRDQHPGILLSLNENIGSTLAGQVANQNLDMAVIYGAKMPAGLQATA
LMREDLYLVATRAVPNPGNSVELLDVARLNLFLPREGDVVRSQVDEAMAIRKLTANVIGEIESPGTLSAAIASGLGATIL
PESVARAMIGPAKAWMARINAPSLSVPLSLCISGQQSLSAPALVVKEILLSIAASRSQEKRVLALVQ

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=306, Percent_Identity=60.4575163398693, Blast_Score=351, Evalue=3e-98,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=27.8884462151394, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=27.3469387755102, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787879, Length=259, Percent_Identity=25.4826254826255, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789204, Length=248, Percent_Identity=25.4032258064516, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=281, Percent_Identity=23.8434163701068, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=27.972027972028, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788887, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=264, Percent_Identity=26.1363636363636, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787589, Length=197, Percent_Identity=29.4416243654822, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693105, Length=237, Percent_Identity=22.3628691983122, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32385; Mature: 32385

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQLATLEGELKQQLLIRTKRGVQPTEA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GNILYAHAQTILRQCEQAQSAVNSAGQAMSGQVSVGLAPGSLASQLALPLLQAVRDQHPG
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCC
ILLSLNENIGSTLAGQVANQNLDMAVIYGAKMPAGLQATALMREDLYLVATRAVPNPGNS
EEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCEEEEEEECCCCCCCC
VELLDVARLNLFLPREGDVVRSQVDEAMAIRKLTANVIGEIESPGTLSAAIASGLGATIL
EEEEEEEEHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHH
PESVARAMIGPAKAWMARINAPSLSVPLSLCISGQQSLSAPALVVKEILLSIAASRSQEK
HHHHHHHHHCHHHHHHHHCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
RVLALVQ
CEEEECC
>Mature Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQLATLEGELKQQLLIRTKRGVQPTEA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GNILYAHAQTILRQCEQAQSAVNSAGQAMSGQVSVGLAPGSLASQLALPLLQAVRDQHPG
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCC
ILLSLNENIGSTLAGQVANQNLDMAVIYGAKMPAGLQATALMREDLYLVATRAVPNPGNS
EEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCEEEEEEECCCCCCCC
VELLDVARLNLFLPREGDVVRSQVDEAMAIRKLTANVIGEIESPGTLSAAIASGLGATIL
EEEEEEEEHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHH
PESVARAMIGPAKAWMARINAPSLSVPLSLCISGQQSLSAPALVVKEILLSIAASRSQEK
HHHHHHHHHCHHHHHHHHCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCC
RVLALVQ
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]