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The map label for this gene is insQ [H]

Identifier: 157371306

GI number: 157371306

Start: 3375170

End: 3376378

Strand: Reverse

Name: insQ [H]

Synonym: Spro_3066

Alternate gene names: 157371306

Gene position: 3376378-3375170 (Counterclockwise)

Preceding gene: 157371310

Following gene: 157371305

Centisome position: 61.96

GC content: 48.22

Gene sequence:

>1209_bases
ATGAAACGACTTCAAGCCTTCAAATTCCAGTTAAGACCCAATGGTCAGCAGGTCCGCGATATGCGGCGCTTCGCTGGGGC
ATGTCGTTTTGTATTCAATAAATCACTCGCCTTGCAGAACGAAAATCATGAGACAGGTAATAAATACCTTCCCTATGTGA
AAATGGCGGCATGGTTGGTTGAGTGGAAAAAAATGCCTGACACAGCATGGTTGAAAGAAGCACCTTCCCAGCCTTTGCAG
CAGGCGTTAAAAGACCTCGAACGCGCCTACAAAAATTTTTTCCAGAAACGGGCATCATTCCCCCGGTTTAAAAAACGCGG
TCAAAGTGACACATTGCGCTACCCGCAGGGTGTGAAACTGGATCAAGAAAACAACCGCATATCGCTGCCCAAACTTGGCT
GGATACACTATCGCAATAGTCGTCAAATTGTGGGCGAGGTTAAAAATGTCACGGTTAGCCAGTCATGTGGCAAGTGGTAT
ATCAGTGTCCAGACTGAATATGAAGCTGATGAATCGGTTCATACGTCAACTTCTATGGTTGGGCTTGATGCTGGCGTAGC
GAAGCTCGCCACGCTATCAGATGGCACTATTTTTGAGCCAGTAAACAGCTTTAAAATCAACCAAAATAAACTCGCCAGGC
TCCAACGTGAAATGAACCGTAAGGTGAAATTCAGCAACAACTGGAAGAAAGCAAAACGTAAAGTACAAAATCTGCATTCC
CGTATCGGTAATATCCGCCGCGACTACCTCCATAAAGTCAGCACGACAATCAGCAAAAACCACGCGATGATCGTCATTGA
GGACTTGAAGGTATCAAATATGTCAAAGTCAGCAGTGGGTACTGAAAGCCAGCCAGGGGGCAATGTCCGGGCAAAATCAG
GCTTAAACCGTTCGATACTGGATCAGGGTTGGTACGAACTCCGTCGGCAGCTTGAGTACAAGCAGTTCTGGCGAGGTGGT
CAGGTACTGGCAATCAATCCAGCCTACACCAGTCAAAAATGCGCCTGCTGTGGTCATACAGCGAAAGAAAACCGGCAATC
ACAAAGTCAGTTCGAGTGTCTGGTATGTGGGTACACCGCGAACGCCGATATAAATGGCGCCCGCAATATTTTAGCGGCAG
GGCATGTCGCGTTAGCCTGTGGAGAGATGGCAGCTTTAGGCCGCTCTATGAAGCAGGAACCCACCGAGGCGAGTCAGACT
TCGGTCTGA

Upstream 100 bases:

>100_bases
TTGGCAACAAAGACCAAGTGAACCTGCATCAGGAAAGTACAGTGTCTTCCTCGGCGAATATCAATTTCTTTTATCATAGG
CCAAGAGTAAAATTTGAAGT

Downstream 100 bases:

>100_bases
ACGCTGTAGGGATCCTCGGCATTTAGGCCGGGGAGGATGTCAATCGTTAATCACCTGGACCACTACATCTTAAACGGTGA
AAATGAATAAGTTGTTGAGG

Product: IS605 family transposase OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKRLQAFKFQLRPNGQQVRDMRRFAGACRFVFNKSLALQNENHETGNKYLPYVKMAAWLVEWKKMPDTAWLKEAPSQPLQ
QALKDLERAYKNFFQKRASFPRFKKRGQSDTLRYPQGVKLDQENNRISLPKLGWIHYRNSRQIVGEVKNVTVSQSCGKWY
ISVQTEYEADESVHTSTSMVGLDAGVAKLATLSDGTIFEPVNSFKINQNKLARLQREMNRKVKFSNNWKKAKRKVQNLHS
RIGNIRRDYLHKVSTTISKNHAMIVIEDLKVSNMSKSAVGTESQPGGNVRAKSGLNRSILDQGWYELRRQLEYKQFWRGG
QVLAINPAYTSQKCACCGHTAKENRQSQSQFECLVCGYTANADINGARNILAAGHVALACGEMAALGRSMKQEPTEASQT
SV

Sequences:

>Translated_402_residues
MKRLQAFKFQLRPNGQQVRDMRRFAGACRFVFNKSLALQNENHETGNKYLPYVKMAAWLVEWKKMPDTAWLKEAPSQPLQ
QALKDLERAYKNFFQKRASFPRFKKRGQSDTLRYPQGVKLDQENNRISLPKLGWIHYRNSRQIVGEVKNVTVSQSCGKWY
ISVQTEYEADESVHTSTSMVGLDAGVAKLATLSDGTIFEPVNSFKINQNKLARLQREMNRKVKFSNNWKKAKRKVQNLHS
RIGNIRRDYLHKVSTTISKNHAMIVIEDLKVSNMSKSAVGTESQPGGNVRAKSGLNRSILDQGWYELRRQLEYKQFWRGG
QVLAINPAYTSQKCACCGHTAKENRQSQSQFECLVCGYTANADINGARNILAAGHVALACGEMAALGRSMKQEPTEASQT
SV
>Mature_402_residues
MKRLQAFKFQLRPNGQQVRDMRRFAGACRFVFNKSLALQNENHETGNKYLPYVKMAAWLVEWKKMPDTAWLKEAPSQPLQ
QALKDLERAYKNFFQKRASFPRFKKRGQSDTLRYPQGVKLDQENNRISLPKLGWIHYRNSRQIVGEVKNVTVSQSCGKWY
ISVQTEYEADESVHTSTSMVGLDAGVAKLATLSDGTIFEPVNSFKINQNKLARLQREMNRKVKFSNNWKKAKRKVQNLHS
RIGNIRRDYLHKVSTTISKNHAMIVIEDLKVSNMSKSAVGTESQPGGNVRAKSGLNRSILDQGWYELRRQLEYKQFWRGG
QVLAINPAYTSQKCACCGHTAKENRQSQSQFECLVCGYTANADINGARNILAAGHVALACGEMAALGRSMKQEPTEASQT
SV

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=381, Percent_Identity=79.7900262467192, Blast_Score=652, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 45689; Mature: 45689

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLQAFKFQLRPNGQQVRDMRRFAGACRFVFNKSLALQNENHETGNKYLPYVKMAAWLV
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHH
EWKKMPDTAWLKEAPSQPLQQALKDLERAYKNFFQKRASFPRFKKRGQSDTLRYPQGVKL
HHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
DQENNRISLPKLGWIHYRNSRQIVGEVKNVTVSQSCGKWYISVQTEYEADESVHTSTSMV
CCCCCEECCCCCEEEEECCCHHHHHHHHCEEEECCCCEEEEEEEECCCCCCCHHCCHHHE
GLDAGVAKLATLSDGTIFEPVNSFKINQNKLARLQREMNRKVKFSNNWKKAKRKVQNLHS
ECCCCHHHHHCCCCCCEECCCHHCEECHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
RIGNIRRDYLHKVSTTISKNHAMIVIEDLKVSNMSKSAVGTESQPGGNVRAKSGLNRSIL
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCEECCCCCCHHHH
DQGWYELRRQLEYKQFWRGGQVLAINPAYTSQKCACCGHTAKENRQSQSQFECLVCGYTA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCHHHHCCCCCCCEEEEEEEEC
NADINGARNILAAGHVALACGEMAALGRSMKQEPTEASQTSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MKRLQAFKFQLRPNGQQVRDMRRFAGACRFVFNKSLALQNENHETGNKYLPYVKMAAWLV
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHH
EWKKMPDTAWLKEAPSQPLQQALKDLERAYKNFFQKRASFPRFKKRGQSDTLRYPQGVKL
HHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
DQENNRISLPKLGWIHYRNSRQIVGEVKNVTVSQSCGKWYISVQTEYEADESVHTSTSMV
CCCCCEECCCCCEEEEECCCHHHHHHHHCEEEECCCCEEEEEEEECCCCCCCHHCCHHHE
GLDAGVAKLATLSDGTIFEPVNSFKINQNKLARLQREMNRKVKFSNNWKKAKRKVQNLHS
ECCCCHHHHHCCCCCCEECCCHHCEECHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
RIGNIRRDYLHKVSTTISKNHAMIVIEDLKVSNMSKSAVGTESQPGGNVRAKSGLNRSIL
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCCCCCCEECCCCCCHHHH
DQGWYELRRQLEYKQFWRGGQVLAINPAYTSQKCACCGHTAKENRQSQSQFECLVCGYTA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCHHHHCCCCCCCEEEEEEEEC
NADINGARNILAAGHVALACGEMAALGRSMKQEPTEASQTSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]