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The map label for this gene is flgJ [H]

Identifier: 157371202

GI number: 157371202

Start: 3261795

End: 3262751

Strand: Reverse

Name: flgJ [H]

Synonym: Spro_2962

Alternate gene names: 157371202

Gene position: 3262751-3261795 (Counterclockwise)

Preceding gene: 157371203

Following gene: 157371201

Centisome position: 59.88

GC content: 59.56

Gene sequence:

>957_bases
ATGGCCAGCGATCTGATGGCGATGTCGGGTGCCGCTTATGATGCGCAGGCGCTAAACGGGCTTAAGCGTGACGCGGCGAG
CGATCCGCAGGGCAATCTGAAACAGGTGGCGCAACAGGTTGAAGGCATGTTCGTGCAGATGATGCTGAAAAGTATGCGTG
CCGCGCTACCCAAGGACGGGGTGCTGAGCAGCGATCAGTCGCGGCTCTATACCTCAATGTATGACCAGCAGATTGCCCAG
CAGATGTCGCAAAAGGGGCTGGGGATGGCCGACATGATGGTTAAGCAGATGAGCAATGCCAACGCGGTGCCGAGTGAGAC
CGCAGGCATGTCGCCAATGGCGCTGGACCTTCTTTATCAGGACAGTGAGGTGCTGCAAACGCTGCCAAATCAGGCGCTGG
AGCAAATGATCCGTCGTGCGGTACCGAAAGCGCCTGCCGCGTCGCCGCTGTCGCTCAATAACGGCAACTTTGTCGCCAGG
TTGTCTGTTCCGGCGCGCGTTGCCAGCCAGCAGAGCGGCATTCCGCATCAGTTGATTGTGGCGCAGGCCGCGCTGGAGTC
GGGTTGGGGCCAGCGTGAGATCCCGACCGCCAACGGTTCGCCGAGCTACAACCTGTTTGGCATCAAGGCCGGCAGCAGCT
GGAGCGGGCCGGTTACCGAGGTCACCACCACCGAGTTTGAACAGGGGGCCGCGAAGAAGATCAAGGCGCAGTTCCGGGTG
TACGGCTCTTACGTTGAAGCCATTGCCGACTACGTCAAGCTGTTGACCAATAACCCACGCTACGCCGAAGTGGCCAATGC
GCGCAGCCCCGAGCAGGCGGCACATGCGTTGCAGAAGGCCGGTTACGCTACCGATCCGCAATACGCCAACAAGCTGGTCA
GCGTGATCCAGCAGATGAAAAACACCGGCGAGCAGGTGGTTAAGGCTTATACCCATGACCTGAAGGATCTGTTCTAA

Upstream 100 bases:

>100_bases
GCGCGCTGAATGCTCTGGGGGCGACACCGATCGATCTGATGTCGATCCTGCAGGCAATGCAAAGTGCCGGCTGTCTGCGT
GCCAAGTTGGAAATCATCTG

Downstream 100 bases:

>100_bases
ATCCCCGTCATTCTTGACGCTGCAGCTGTGTTGGCTGCACAAGCTCACCCCGGTCACTTGGCAGACCAAGCTTCCGGGGA
CTCCCTTGCTGGCCGCCGTG

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MASDLMAMSGAAYDAQALNGLKRDAASDPQGNLKQVAQQVEGMFVQMMLKSMRAALPKDGVLSSDQSRLYTSMYDQQIAQ
QMSQKGLGMADMMVKQMSNANAVPSETAGMSPMALDLLYQDSEVLQTLPNQALEQMIRRAVPKAPAASPLSLNNGNFVAR
LSVPARVASQQSGIPHQLIVAQAALESGWGQREIPTANGSPSYNLFGIKAGSSWSGPVTEVTTTEFEQGAAKKIKAQFRV
YGSYVEAIADYVKLLTNNPRYAEVANARSPEQAAHALQKAGYATDPQYANKLVSVIQQMKNTGEQVVKAYTHDLKDLF

Sequences:

>Translated_318_residues
MASDLMAMSGAAYDAQALNGLKRDAASDPQGNLKQVAQQVEGMFVQMMLKSMRAALPKDGVLSSDQSRLYTSMYDQQIAQ
QMSQKGLGMADMMVKQMSNANAVPSETAGMSPMALDLLYQDSEVLQTLPNQALEQMIRRAVPKAPAASPLSLNNGNFVAR
LSVPARVASQQSGIPHQLIVAQAALESGWGQREIPTANGSPSYNLFGIKAGSSWSGPVTEVTTTEFEQGAAKKIKAQFRV
YGSYVEAIADYVKLLTNNPRYAEVANARSPEQAAHALQKAGYATDPQYANKLVSVIQQMKNTGEQVVKAYTHDLKDLF
>Mature_317_residues
ASDLMAMSGAAYDAQALNGLKRDAASDPQGNLKQVAQQVEGMFVQMMLKSMRAALPKDGVLSSDQSRLYTSMYDQQIAQQ
MSQKGLGMADMMVKQMSNANAVPSETAGMSPMALDLLYQDSEVLQTLPNQALEQMIRRAVPKAPAASPLSLNNGNFVARL
SVPARVASQQSGIPHQLIVAQAALESGWGQREIPTANGSPSYNLFGIKAGSSWSGPVTEVTTTEFEQGAAKKIKAQFRVY
GSYVEAIADYVKLLTNNPRYAEVANARSPEQAAHALQKAGYATDPQYANKLVSVIQQMKNTGEQVVKAYTHDLKDLF

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=319, Percent_Identity=59.2476489028213, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34328; Mature: 34197

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDLMAMSGAAYDAQALNGLKRDAASDPQGNLKQVAQQVEGMFVQMMLKSMRAALPKDG
CCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLSSDQSRLYTSMYDQQIAQQMSQKGLGMADMMVKQMSNANAVPSETAGMSPMALDLLYQ
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHC
DSEVLQTLPNQALEQMIRRAVPKAPAASPLSLNNGNFVARLSVPARVASQQSGIPHQLIV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEECCCHHHHHHCCCCCHHHHH
AQAALESGWGQREIPTANGSPSYNLFGIKAGSSWSGPVTEVTTTEFEQGAAKKIKAQFRV
HHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YGSYVEAIADYVKLLTNNPRYAEVANARSPEQAAHALQKAGYATDPQYANKLVSVIQQMK
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NTGEQVVKAYTHDLKDLF
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASDLMAMSGAAYDAQALNGLKRDAASDPQGNLKQVAQQVEGMFVQMMLKSMRAALPKDG
CCCHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLSSDQSRLYTSMYDQQIAQQMSQKGLGMADMMVKQMSNANAVPSETAGMSPMALDLLYQ
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHC
DSEVLQTLPNQALEQMIRRAVPKAPAASPLSLNNGNFVARLSVPARVASQQSGIPHQLIV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEECCCHHHHHHCCCCCHHHHH
AQAALESGWGQREIPTANGSPSYNLFGIKAGSSWSGPVTEVTTTEFEQGAAKKIKAQFRV
HHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YGSYVEAIADYVKLLTNNPRYAEVANARSPEQAAHALQKAGYATDPQYANKLVSVIQQMK
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NTGEQVVKAYTHDLKDLF
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388 [H]