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The map label for this gene is pykA [H]

Identifier: 157371010

GI number: 157371010

Start: 3050023

End: 3051465

Strand: Direct

Name: pykA [H]

Synonym: Spro_2770

Alternate gene names: 157371010

Gene position: 3050023-3051465 (Clockwise)

Preceding gene: 157371009

Following gene: 157371014

Centisome position: 55.98

GC content: 56.69

Gene sequence:

>1443_bases
ATGTCCAGACGGCTCAGAAGAACCAAAATTGTTACCACCCTGGGTCCGGCTACAGACCGCGACAATAATCTGGAAAAGAT
CATTGCCGCCGGCGCTAACGTAGTTCGGCTTAACTTTTCCCACGGCACCGCAGAAGACCACCAGATCCGCGCTGACAAAG
TGCGCGAAATTGCTGCCAAGTTGGGACGCCACGTCGCTATTCTGGGTGACCTGCAAGGGCCGAAAATTCGTGTTTCCACC
TTTAAGGAAGGCAAAATCTTCCTTAACATCGGTGATAAATTCCTGCTTGATGCCAACATGTCCAAGGGCGAAGGCGATAA
AGAAAAAGTCGGTATCGACTATAAAGGCCTGCCTGCCGACGTGGTACCCGGCGACGTACTGCTGCTCGATGACGGCCGCG
TACAGCTGAAAGTGCTCGAAGTTCAGGGCATGAAAGTGTTTACCGAAGTTACCGTTGGCGGCCCACTGTCCAACAACAAA
GGTATCAACAAACTGGGTGGCGGCCTGTCCGCTGAAGCCCTGACCGAGAAAGACAAGGCCGATATCGTCACCGCGGCTAA
AATCGGCGTCGACTACCTGGCGGTTTCCTTCCCGCGTACCGGCGAAGATTTGAACTACGCACGTCGACTGGCGCGCGATG
CCGGCTGCAACGCCAAGATCGTGTCCAAGGTTGAACGTGCCGAAGCCGTTTGCAGCGACGAAGCCATGGATGACATCATC
CTGGCCTCCGACGTCGTGATGGTAGCCCGTGGCGACCTGGGCGTCGAAATCGGCGATCCGGAACTGGTCGGCATCCAGAA
AAAACTGATCCGCCGTGCCCGTACGCTCAACCGCGCCGTCATTACCGCGACCCAGATGATGGAGTCGATGATCACCAACC
CGATGCCTACCCGTGCCGAAGTCATGGACGTGGCAAACGCCGTGCTGGATGGTACCGACGCCGTGATGCTGTCTGCTGAA
ACCGCGGCAGGCCAGTACCCGGCAGAAACCGTGGCGGCTATGGCGCGTGTTTGTCTGGGTGCAGAGAAAATCCCAAGCAT
CAACGTCTCCAAACACCGTCTGGACGTGCAGTTCGACAACATTGAAGAAGCCATTGCCATGTCTTCCATGTACGCGGCTA
ACCATCTGAAAGGGGTTACCGCACTGATCGCCATGACCGAGTCAGGCCGCACCGCGCTGATGATGTCACGTATCAGTTCC
GGTCTGCCGATCTTCGCCATGTCACGCCATGAGCACACGTTGAACCTGACTGCGCTGTACCGCGGCGTTACGCCAGTGTA
CTTCGACAGCCATAATGACGGTGTTATCGCGGCCAATGATGCGGTTAACCGCCTGCGTGATAAAGGCTTCCTGGTCTCCG
GCGATCTGGTGATCGTCACCCAGGGCGACGTGATGGAAACCGTGGGTACCACCAATACCAGCCGTATTCTGCGCGTCGAG
TAA

Upstream 100 bases:

>100_bases
GTGAAATATCAGTGTAGTAAAATGACATTTCACTCCTGGTTTCGTTATCCGACGTTATCGCAACACCAATAATTTGCTTC
ACCAGTCAACGGAGTAATAC

Downstream 100 bases:

>100_bases
AATATACTGCTGCCGGCCGTTAACACGGTCGGCATTTTTTACGCTCCCCCCTTCCCCCTGAGAAACTCCCCTCTAATCGC
CGTTACGGCAAATGGAGAGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGTAEDHQIRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKIFLNIGDKFLLDANMSKGEGDKEKVGIDYKGLPADVVPGDVLLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIVTAAKIGVDYLAVSFPRTGEDLNYARRLARDAGCNAKIVSKVERAEAVCSDEAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNIEEAIAMSSMYAANHLKGVTALIAMTESGRTALMMSRISS
GLPIFAMSRHEHTLNLTALYRGVTPVYFDSHNDGVIAANDAVNRLRDKGFLVSGDLVIVTQGDVMETVGTTNTSRILRVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGTAEDHQIRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKIFLNIGDKFLLDANMSKGEGDKEKVGIDYKGLPADVVPGDVLLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIVTAAKIGVDYLAVSFPRTGEDLNYARRLARDAGCNAKIVSKVERAEAVCSDEAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNIEEAIAMSSMYAANHLKGVTALIAMTESGRTALMMSRISS
GLPIFAMSRHEHTLNLTALYRGVTPVYFDSHNDGVIAANDAVNRLRDKGFLVSGDLVIVTQGDVMETVGTTNTSRILRVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGTAEDHQIRADKVREIAAKLGRHVAILGDLQGPKIRVSTF
KEGKIFLNIGDKFLLDANMSKGEGDKEKVGIDYKGLPADVVPGDVLLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKG
INKLGGGLSAEALTEKDKADIVTAAKIGVDYLAVSFPRTGEDLNYARRLARDAGCNAKIVSKVERAEAVCSDEAMDDIIL
ASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNIEEAIAMSSMYAANHLKGVTALIAMTESGRTALMMSRISSG
LPIFAMSRHEHTLNLTALYRGVTPVYFDSHNDGVIAANDAVNRLRDKGFLVSGDLVIVTQGDVMETVGTTNTSRILRVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=38.0478087649402, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=38.0478087649402, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=37.3737373737374, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=35.5555555555556, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=35.5555555555556, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=36.2068965517241, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=36.2068965517241, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.4430379746835, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.4430379746835, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=92.0833333333333, Blast_Score=901, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=483, Percent_Identity=38.0952380952381, Blast_Score=288, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=492, Percent_Identity=36.3821138211382, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17506831, Length=495, Percent_Identity=37.3737373737374, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=495, Percent_Identity=37.3737373737374, Blast_Score=241, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=495, Percent_Identity=37.3737373737374, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=495, Percent_Identity=37.3737373737374, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=473, Percent_Identity=35.7293868921776, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=496, Percent_Identity=34.6774193548387, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.246963562753, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.246963562753, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24648966, Length=454, Percent_Identity=33.920704845815, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24581235, Length=436, Percent_Identity=32.5688073394495, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=35.1851851851852, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51636; Mature: 51505

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGTAEDHQIRADKVREIAAK
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKIFLNIGDKFLLDANMSKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCHHCCCCCCCCCCC
VVPGDVLLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCCHH
DIVTAAKIGVDYLAVSFPRTGEDLNYARRLARDAGCNAKIVSKVERAEAVCSDEAMDDII
CEEEEHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHCCH
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
IEEAIAMSSMYAANHLKGVTALIAMTESGRTALMMSRISSGLPIFAMSRHEHTLNLTALY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEHC
RGVTPVYFDSHNDGVIAANDAVNRLRDKGFLVSGDLVIVTQGDVMETVGTTNTSRILRVE
CCCCEEEEECCCCCEEEECHHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGTAEDHQIRADKVREIAAK
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKIFLNIGDKFLLDANMSKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCHHCCCCCCCCCCC
VVPGDVLLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCCHH
DIVTAAKIGVDYLAVSFPRTGEDLNYARRLARDAGCNAKIVSKVERAEAVCSDEAMDDII
CEEEEHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHCCH
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARTLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
IEEAIAMSSMYAANHLKGVTALIAMTESGRTALMMSRISSGLPIFAMSRHEHTLNLTALY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEHC
RGVTPVYFDSHNDGVIAANDAVNRLRDKGFLVSGDLVIVTQGDVMETVGTTNTSRILRVE
CCCCEEEEECCCCCEEEECHHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]