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The map label for this gene is purU [H]

Identifier: 157370953

GI number: 157370953

Start: 2989603

End: 2990451

Strand: Direct

Name: purU [H]

Synonym: Spro_2713

Alternate gene names: 157370953

Gene position: 2989603-2990451 (Clockwise)

Preceding gene: 157370952

Following gene: 157370954

Centisome position: 54.87

GC content: 51.24

Gene sequence:

>849_bases
ATGCCACACCAAAATGTACAAAGAAAAGTTTTGCGCACCATTTGTCCGGATGCCAAAGGCCTGATCGCCAAGATCACCAA
TATTTGTTACAAACATGAACTCAACATTGTTCAGAACAATGAATTCGTTGATCACCGTACTGGCCGCTTCTTTATGCGTA
CCGAGCTGGAAGGCATTTTTAACGACACTACTTTGCTGGCGGATCTCGATAGCGCACTGCCGGTGGGTTCGGTCCGCGAA
CTGCATAACTCCGGCCGTCGTCGTATTGTGGTGCTGGTGACCAAAGAAGCGCATTGCCTGGGCGACTTGCTGATGAAAAG
CGCTTACGGCGGCCTGGATGTGGAAATTGCGGCGGTTATCGGTAACCACGATACGCTGCAAACGTTGGTGGAACGTTTCG
ATATCCCGTTCCACCTGGTCAGCCATGAAGGCCTGACGCGCGATCAACACGATCAGAAAATGATGGCCCAGATCGATCAG
TATCAGCCTGATTACGTGGTGTTGGCCAAATATATGCGCGTACTGACGCCAGCATTTGTACAGCACTACCCGAACCAGGT
GATTAATATTCACCACTCGTTCCTGCCGGCCTTTATCGGTGCACGGCCTTACCATCAGGCTTACGAACGTGGCGTGAAAA
TCATCGGCGCTACGGCCCACTACGTTAACGATAATCTGGACGAAGGCCCGATCATCATGCAGGACGTGATTCATGTCGAT
CACACCTATTCTGCTGAAGATATGATGCGTGCCGGACGAGACGTCGAGAAAAATGCGCTCAGCCGCGCGCTTTACCATGT
GTTGGCCCAACGGGTATTTGTCTACGGTAACCGGACGGTTATTCTGTAA

Upstream 100 bases:

>100_bases
TCAGGTCGCAGCCAATCCGGTCGCCGCTTGAAAATATAGATGATAAAAACTCTCGCCTGCGAAGACAGGACGTTCAGTAT
CAGACAGGATCCTCACTCCC

Downstream 100 bases:

>100_bases
TTGCTCAAGAAAGCGGTGCCTTTCGTTCAGGGATGCACCGCCACGAGTAAAAAATCGGCAAACGATTCATTTTCTGCATT
TCAATACTTTACACGCCCGT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPHQNVQRKVLRTICPDAKGLIAKITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDTTLLADLDSALPVGSVRE
LHNSGRRRIVVLVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDTLQTLVERFDIPFHLVSHEGLTRDQHDQKMMAQIDQ
YQPDYVVLAKYMRVLTPAFVQHYPNQVINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVD
HTYSAEDMMRAGRDVEKNALSRALYHVLAQRVFVYGNRTVIL

Sequences:

>Translated_282_residues
MPHQNVQRKVLRTICPDAKGLIAKITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDTTLLADLDSALPVGSVRE
LHNSGRRRIVVLVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDTLQTLVERFDIPFHLVSHEGLTRDQHDQKMMAQIDQ
YQPDYVVLAKYMRVLTPAFVQHYPNQVINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVD
HTYSAEDMMRAGRDVEKNALSRALYHVLAQRVFVYGNRTVIL
>Mature_281_residues
PHQNVQRKVLRTICPDAKGLIAKITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDTTLLADLDSALPVGSVREL
HNSGRRRIVVLVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDTLQTLVERFDIPFHLVSHEGLTRDQHDQKMMAQIDQY
QPDYVVLAKYMRVLTPAFVQHYPNQVINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDH
TYSAEDMMRAGRDVEKNALSRALYHVLAQRVFVYGNRTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=157, Percent_Identity=31.8471337579618, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI209869995, Length=157, Percent_Identity=31.8471337579618, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI209869993, Length=157, Percent_Identity=31.8471337579618, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=88.8489208633094, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1788846, Length=103, Percent_Identity=42.7184466019417, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=196, Percent_Identity=29.0816326530612, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=28.2608695652174, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32146; Mature: 32015

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHQNVQRKVLRTICPDAKGLIAKITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIF
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHH
NDTTLLADLDSALPVGSVRELHNSGRRRIVVLVTKEAHCLGDLLMKSAYGGLDVEIAAVI
CCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEE
GNHDTLQTLVERFDIPFHLVSHEGLTRDQHDQKMMAQIDQYQPDYVVLAKYMRVLTPAFV
CCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QHYPNQVINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHC
HTYSAEDMMRAGRDVEKNALSRALYHVLAQRVFVYGNRTVIL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
PHQNVQRKVLRTICPDAKGLIAKITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHH
NDTTLLADLDSALPVGSVRELHNSGRRRIVVLVTKEAHCLGDLLMKSAYGGLDVEIAAVI
CCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEE
GNHDTLQTLVERFDIPFHLVSHEGLTRDQHDQKMMAQIDQYQPDYVVLAKYMRVLTPAFV
CCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QHYPNQVINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHC
HTYSAEDMMRAGRDVEKNALSRALYHVLAQRVFVYGNRTVIL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]