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The map label for this gene is cls

Identifier: 157370929

GI number: 157370929

Start: 2960099

End: 2961559

Strand: Direct

Name: cls

Synonym: Spro_2689

Alternate gene names: 157370929

Gene position: 2960099-2961559 (Clockwise)

Preceding gene: 157370928

Following gene: 157370930

Centisome position: 54.33

GC content: 54.28

Gene sequence:

>1461_bases
ATGACAACATTTTATACCGTAATCAGTTGGCTCATGGTGTTCGGTTACTGGCTGCTTATCGCTGGTGTGACACTGCGTAT
TCTGATGAAACGCCGCGCCGTACCTTCCGCTATGGCCTGGCTGCTGGTGATCTATATTCTGCCGCTGTTCGGTATTGTGG
CTTATTTATCTTTTGGTGAATTACATCTGGGCAAACGGCGTGCCGAACGCGCCAAGGCAATGTGGCCTTCTACCGCACGC
TGGCTGAGCGAGTTAAAAGAGAGCCAGCGCATCTTCGCTACCGATTACAGCGAGGTCGCCAGACCCCTGTTCCAACTCTG
CGATCGCCGTCAGGGCATCGATGGCGTTAAGGGCAACCAACTCCAGTTACTCACCACTACCGATGCAACGCTGAAGGCGC
TGATCCGTGATATTGAGCTGGCACGCCATAATATAGAGATGGTGTTTTACATTTGGCAGCCCGGCGGGTTGGTCGATCAG
GTAGCCGAATCGCTGATGGCCGCTGCACGCCGTGGCGTACATTGCCGCCTGATGCTGGACTCTGCCGGTAGTTTGCAGTT
CTTCCGCAGCCCCTATCCTGGCATGATGCGCAATGCCGGTATCGAGGTGGTGGAGGCACTCAAGGTAAACCTGTTCCGCG
TTTTCCTGCGCCGAATGGATTTGCGTCAGCACCGTAAAGTGGTGCTGATTGACAACTATATTGCCTACACCGGCAGTATG
AACATGGTCGACCCACGCTATTTCAAACAGGATGCCGGGGTCGGTCAGTGGATCGATTTGATGGCCCGTATGGAAGGCCC
GGTCGCCAGTACCATGGGCATCGTTTATGCCTGTGACTGGGAGATTGAAACCGGTAAACGGATCCTGCCGCCGCCGCCGG
ACGTCAATATCATGCCGTTCGAACAGGAAAGCGGCCACACTATTCAGGTTATCGCCTCTGGCCCCGGGTTCCCGGAAGAA
ATGATCCACCAGGCGTTGCTTACTGCGGTTTACTCAGCACGTGAACAGTTGATCATGACCACCCCCTACTTCGTACCCAG
CGACGATTTACTGCATGCGATTTGCACCGCCGCACTGCGCGGGGTTGAAGTCAGCATCATTGTCCCACGCGATAACGATT
CAACGATGGTCCGTTGGGCCAGTCGCTCTTTCTTCTCCGAACTTCTGGAGGCTGGCGTACGCATCTATCAGTTTGAAGAC
GGCCTGCTGCATACCAAAAGCGTACTGGTTGATGGCCAGCTCAGCCTGGTCGGCACGGTGAATTTGGATATGCGCAGCCT
GTGGTTGAACTTTGAAATTACCCTGGTGATTGACGATGACGGTTTCGGTAGCGATCTGGCCTGCGTGCAGGACGACTATA
TTGCTCGTTCGCAGTTATTGAATGCCGCAGAGTGGCAGAAACGGCCTTTTTGGCACCGTATTGTTGAGCGCCTGTTCTAC
TTTTTCAGCCCGCTGTTATAA

Upstream 100 bases:

>100_bases
ATCATATTTTTGCAACATAAGCGCCGAGAAAAGGTTTCCCCTTTAGCGTTCTGTGCGTACACTCATCGTTGGTGGTTTGC
GAACGGGATTTTTCGCCTTT

Downstream 100 bases:

>100_bases
ACGCCGTAACGGGCATGCTTCATACCCGTCGCCTTTCAAGTTGCAGCGTTGTTACCTGCACTCGGTTATGTGGCCCGTCC
ATGGGCCACCCCCCTTCGGG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTFYTVISWLMVFGYWLLIAGVTLRILMKRRAVPSAMAWLLVIYILPLFGIVAYLSFGELHLGKRRAERAKAMWPSTAR
WLSELKESQRIFATDYSEVARPLFQLCDRRQGIDGVKGNQLQLLTTTDATLKALIRDIELARHNIEMVFYIWQPGGLVDQ
VAESLMAAARRGVHCRLMLDSAGSLQFFRSPYPGMMRNAGIEVVEALKVNLFRVFLRRMDLRQHRKVVLIDNYIAYTGSM
NMVDPRYFKQDAGVGQWIDLMARMEGPVASTMGIVYACDWEIETGKRILPPPPDVNIMPFEQESGHTIQVIASGPGFPEE
MIHQALLTAVYSAREQLIMTTPYFVPSDDLLHAICTAALRGVEVSIIVPRDNDSTMVRWASRSFFSELLEAGVRIYQFED
GLLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGSDLACVQDDYIARSQLLNAAEWQKRPFWHRIVERLFY
FFSPLL

Sequences:

>Translated_486_residues
MTTFYTVISWLMVFGYWLLIAGVTLRILMKRRAVPSAMAWLLVIYILPLFGIVAYLSFGELHLGKRRAERAKAMWPSTAR
WLSELKESQRIFATDYSEVARPLFQLCDRRQGIDGVKGNQLQLLTTTDATLKALIRDIELARHNIEMVFYIWQPGGLVDQ
VAESLMAAARRGVHCRLMLDSAGSLQFFRSPYPGMMRNAGIEVVEALKVNLFRVFLRRMDLRQHRKVVLIDNYIAYTGSM
NMVDPRYFKQDAGVGQWIDLMARMEGPVASTMGIVYACDWEIETGKRILPPPPDVNIMPFEQESGHTIQVIASGPGFPEE
MIHQALLTAVYSAREQLIMTTPYFVPSDDLLHAICTAALRGVEVSIIVPRDNDSTMVRWASRSFFSELLEAGVRIYQFED
GLLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGSDLACVQDDYIARSQLLNAAEWQKRPFWHRIVERLFY
FFSPLL
>Mature_485_residues
TTFYTVISWLMVFGYWLLIAGVTLRILMKRRAVPSAMAWLLVIYILPLFGIVAYLSFGELHLGKRRAERAKAMWPSTARW
LSELKESQRIFATDYSEVARPLFQLCDRRQGIDGVKGNQLQLLTTTDATLKALIRDIELARHNIEMVFYIWQPGGLVDQV
AESLMAAARRGVHCRLMLDSAGSLQFFRSPYPGMMRNAGIEVVEALKVNLFRVFLRRMDLRQHRKVVLIDNYIAYTGSMN
MVDPRYFKQDAGVGQWIDLMARMEGPVASTMGIVYACDWEIETGKRILPPPPDVNIMPFEQESGHTIQVIASGPGFPEEM
IHQALLTAVYSAREQLIMTTPYFVPSDDLLHAICTAALRGVEVSIIVPRDNDSTMVRWASRSFFSELLEAGVRIYQFEDG
LLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGSDLACVQDDYIARSQLLNAAEWQKRPFWHRIVERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=82.3045267489712, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=384, Percent_Identity=26.8229166666667, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS_SERP5 (A8GFA0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478918.1
- ProteinModelPortal:   A8GFA0
- STRING:   A8GFA0
- GeneID:   5606471
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2689
- eggNOG:   COG1502
- HOGENOM:   HBG694312
- OMA:   SKKVKMA
- ProtClustDB:   PRK01642
- BioCyc:   SPRO399741:SPRO_2689-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 55395; Mature: 55264

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 224-224 ACT_SITE 226-226 ACT_SITE 231-231 ACT_SITE 404-404 ACT_SITE 406-406 ACT_SITE 411-411

Signals:

None

Transmembrane regions:

HASH(0x2557e8fc)-; HASH(0x241f86d4)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFYTVISWLMVFGYWLLIAGVTLRILMKRRAVPSAMAWLLVIYILPLFGIVAYLSFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHLGKRRAERAKAMWPSTARWLSELKESQRIFATDYSEVARPLFQLCDRRQGIDGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCE
LQLLTTTDATLKALIRDIELARHNIEMVFYIWQPGGLVDQVAESLMAAARRGVHCRLMLD
EEEEEECHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEC
SAGSLQFFRSPYPGMMRNAGIEVVEALKVNLFRVFLRRMDLRQHRKVVLIDNYIAYTGSM
CCCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRYFKQDAGVGQWIDLMARMEGPVASTMGIVYACDWEIETGKRILPPPPDVNIMPF
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHCCEEEEEEEEECCCCCCCCCCCCCEEEEC
EQESGHTIQVIASGPGFPEEMIHQALLTAVYSAREQLIMTTPYFVPSDDLLHAICTAALR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHC
GVEVSIIVPRDNDSTMVRWASRSFFSELLEAGVRIYQFEDGLLHTKSVLVDGQLSLVGTV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEHHEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDGFGSDLACVQDDYIARSQLLNAAEWQKRPFWHRIVERLFY
CCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FFSPLL
HHHCCH
>Mature Secondary Structure 
TTFYTVISWLMVFGYWLLIAGVTLRILMKRRAVPSAMAWLLVIYILPLFGIVAYLSFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHLGKRRAERAKAMWPSTARWLSELKESQRIFATDYSEVARPLFQLCDRRQGIDGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCE
LQLLTTTDATLKALIRDIELARHNIEMVFYIWQPGGLVDQVAESLMAAARRGVHCRLMLD
EEEEEECHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEC
SAGSLQFFRSPYPGMMRNAGIEVVEALKVNLFRVFLRRMDLRQHRKVVLIDNYIAYTGSM
CCCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRYFKQDAGVGQWIDLMARMEGPVASTMGIVYACDWEIETGKRILPPPPDVNIMPF
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHCCEEEEEEEEECCCCCCCCCCCCCEEEEC
EQESGHTIQVIASGPGFPEEMIHQALLTAVYSAREQLIMTTPYFVPSDDLLHAICTAALR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHC
GVEVSIIVPRDNDSTMVRWASRSFFSELLEAGVRIYQFEDGLLHTKSVLVDGQLSLVGTV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEHHEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDGFGSDLACVQDDYIARSQLLNAAEWQKRPFWHRIVERLFY
CCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FFSPLL
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA