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The map label for this gene is braE [H]

Identifier: 157370790

GI number: 157370790

Start: 2806023

End: 2807135

Strand: Direct

Name: braE [H]

Synonym: Spro_2550

Alternate gene names: 157370790

Gene position: 2806023-2807135 (Clockwise)

Preceding gene: 157370789

Following gene: 157370791

Centisome position: 51.5

GC content: 59.12

Gene sequence:

>1113_bases
ATGACTATTCTGACCGCTACTCTACACACCGCTTCCCCGCATCGCGCCAGACTTGGTGTGGTTCTGTTTATGCTCGGTCT
GCTGCTGGCCCCCTGGGTGGCCGGATCCGTCGGCGGTAACTATTGGGTGCGGGTGATCGACTTCGCGCTGCTGTATCTGA
TGCTGGCGCTGGGTCTGAACATCGTGGTCGGCCTCACCGGCCTGCTGGACATGGGATTTATCGCTTTTTACGCCGTCGGC
GCCTACCTGACCGCACTGCTCTCGTCACCGCACCTTACTCAACAATTTCCGCTGCTGTTGCAGTGGTTCCCTGAGGGGCT
GCATCTGTCGATCCTGCTGTTGATCCCACTGGCAGCGGTGCTGGCAGCGCTGTGCGGCATTCTGCTGGGAGCACCTACGC
TGCGGTTGCGTGGCGACTATCTGGCGATCGTCACGCTGGGTTTCGGTGAGATAGTGCGTATTTTGATGCGTAATCTGGAC
CGGCCACTCAATATCACCAATGGTCCTAAAGGGATTTCCGCTATCGATCCGGTCACGCTGTTCGGCCTGAAGTTTTCCGG
TATGCACTCGTGGTTTGGCATCCGTTTTTCATCGCTGTATCTCTATTATTACCTGTTCGCTGCTCTGCTGTTGCTGATCC
TGTTTGTCTGCCTGCGCCTGCAGCATTCGCGGGTTGGCCGCGCCTGGACCGCCATTCGCGAAGATGAAGACGCGGCGCGG
GCGATGGGCATCAACACACGCAACTTCAAGCTGCTGGCTTTCGCCATCGGAGCCACTTTTGGCGGTGTCGCCGGCGCACT
GTTTGCGGCGTTTCAGGGCTTTGTCTCACCGGAATCCTTCACTCTGCAAGAGTCGATCGCCGTATTGGCAATGGTGGTGT
TGGGCGGCATGGGCCACATACCCGGCGTGATCCTCGGCGCATTGCTGCTGGCCGCCCTGCCAGAACTGCTGCGCAGCACC
ATGGGCCCTCTGCAACAGGCGCTATTCGGCCAGGTGTGGATAGATCCAGAGATTATTCGCCAACTGTTCTACGGGCTGGC
CCTAATTCTGGTGATGCTGTATCGCCCTGCGGGCCTGTGGCCGGCACGCCATCAACCGGGAGCCGCTTCATGA

Upstream 100 bases:

>100_bases
TCAGGACGTGTTTGCCTTCCTGATCTTGATTGCCGTACTGGTATTGCGCCCATCCGGGCTGTTGGGCGAACGCGTCGCCA
CCCGCGCCTGAGGACACGAG

Downstream 100 bases:

>100_bases
CGCCACTTCTCACACTCAGTCACGTCAGTAAGCGCTTTGGCGGGCTGACCGCGGTGGATGACGTCAGCATCAGCATTCAC
CAGGGCGAGATTTACGGGTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTILTATLHTASPHRARLGVVLFMLGLLLAPWVAGSVGGNYWVRVIDFALLYLMLALGLNIVVGLTGLLDMGFIAFYAVG
AYLTALLSSPHLTQQFPLLLQWFPEGLHLSILLLIPLAAVLAALCGILLGAPTLRLRGDYLAIVTLGFGEIVRILMRNLD
RPLNITNGPKGISAIDPVTLFGLKFSGMHSWFGIRFSSLYLYYYLFAALLLLILFVCLRLQHSRVGRAWTAIREDEDAAR
AMGINTRNFKLLAFAIGATFGGVAGALFAAFQGFVSPESFTLQESIAVLAMVVLGGMGHIPGVILGALLLAALPELLRST
MGPLQQALFGQVWIDPEIIRQLFYGLALILVMLYRPAGLWPARHQPGAAS

Sequences:

>Translated_370_residues
MTILTATLHTASPHRARLGVVLFMLGLLLAPWVAGSVGGNYWVRVIDFALLYLMLALGLNIVVGLTGLLDMGFIAFYAVG
AYLTALLSSPHLTQQFPLLLQWFPEGLHLSILLLIPLAAVLAALCGILLGAPTLRLRGDYLAIVTLGFGEIVRILMRNLD
RPLNITNGPKGISAIDPVTLFGLKFSGMHSWFGIRFSSLYLYYYLFAALLLLILFVCLRLQHSRVGRAWTAIREDEDAAR
AMGINTRNFKLLAFAIGATFGGVAGALFAAFQGFVSPESFTLQESIAVLAMVVLGGMGHIPGVILGALLLAALPELLRST
MGPLQQALFGQVWIDPEIIRQLFYGLALILVMLYRPAGLWPARHQPGAAS
>Mature_369_residues
TILTATLHTASPHRARLGVVLFMLGLLLAPWVAGSVGGNYWVRVIDFALLYLMLALGLNIVVGLTGLLDMGFIAFYAVGA
YLTALLSSPHLTQQFPLLLQWFPEGLHLSILLLIPLAAVLAALCGILLGAPTLRLRGDYLAIVTLGFGEIVRILMRNLDR
PLNITNGPKGISAIDPVTLFGLKFSGMHSWFGIRFSSLYLYYYLFAALLLLILFVCLRLQHSRVGRAWTAIREDEDAARA
MGINTRNFKLLAFAIGATFGGVAGALFAAFQGFVSPESFTLQESIAVLAMVVLGGMGHIPGVILGALLLAALPELLRSTM
GPLQQALFGQVWIDPEIIRQLFYGLALILVMLYRPAGLWPARHQPGAAS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=331, Percent_Identity=45.3172205438066, Blast_Score=214, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 40082; Mature: 39950

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILTATLHTASPHRARLGVVLFMLGLLLAPWVAGSVGGNYWVRVIDFALLYLMLALGLN
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IVVGLTGLLDMGFIAFYAVGAYLTALLSSPHLTQQFPLLLQWFPEGLHLSILLLIPLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LAALCGILLGAPTLRLRGDYLAIVTLGFGEIVRILMRNLDRPLNITNGPKGISAIDPVTL
HHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
FGLKFSGMHSWFGIRFSSLYLYYYLFAALLLLILFVCLRLQHSRVGRAWTAIREDEDAAR
HHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AMGINTRNFKLLAFAIGATFGGVAGALFAAFQGFVSPESFTLQESIAVLAMVVLGGMGHI
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCH
PGVILGALLLAALPELLRSTMGPLQQALFGQVWIDPEIIRQLFYGLALILVMLYRPAGLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PARHQPGAAS
CCCCCCCCCC
>Mature Secondary Structure 
TILTATLHTASPHRARLGVVLFMLGLLLAPWVAGSVGGNYWVRVIDFALLYLMLALGLN
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IVVGLTGLLDMGFIAFYAVGAYLTALLSSPHLTQQFPLLLQWFPEGLHLSILLLIPLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LAALCGILLGAPTLRLRGDYLAIVTLGFGEIVRILMRNLDRPLNITNGPKGISAIDPVTL
HHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
FGLKFSGMHSWFGIRFSSLYLYYYLFAALLLLILFVCLRLQHSRVGRAWTAIREDEDAAR
HHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AMGINTRNFKLLAFAIGATFGGVAGALFAAFQGFVSPESFTLQESIAVLAMVVLGGMGHI
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCH
PGVILGALLLAALPELLRSTMGPLQQALFGQVWIDPEIIRQLFYGLALILVMLYRPAGLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PARHQPGAAS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]