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The map label for this gene is kefF [H]

Identifier: 157370700

GI number: 157370700

Start: 2694616

End: 2695392

Strand: Direct

Name: kefF [H]

Synonym: Spro_2460

Alternate gene names: 157370700

Gene position: 2694616-2695392 (Clockwise)

Preceding gene: 157370698

Following gene: 157370703

Centisome position: 49.45

GC content: 54.57

Gene sequence:

>777_bases
ATGAATGTTTTACTGGTCTATGCTCACCCTGAACCGCAGTCTTTAAATGGCTCCCTTAAGGATTTATCGGTCCAACGTTT
GAAAGCTGCTGGGCACCAGGTACAGGTTTCCGATCTGTATGCCATGCAATGGAAAGCCACGCTCGATGCCAATGACAGCA
GGGTTCCCCCGCTAGGATCACGCTTTGATCCCTCACTCGATTCCCAGCATGCCTTTGCCAACGGGTTACAGAGCCAGGAC
ATTGAGCTGGAGCAGGAAAAACTACGCTGGGCGGATACGGTCATTTTGCAGTTTCCCCTGTGGTGGTTTTCCATGCCGGC
AATCCTTAAAGGCTGGGTTGAACGCGTCTATGCCTACGGATTTGCCTATGGCGTCGGGGAACACTCTGACTCACGCTGGG
GTGATCGTTATGGCGAAGGCACCCTGGCGGGGAAACGGGCAATGCTGATTGTTACCACCGGTGGTTGGGAATCTCATTAC
GCTGCGAGGGGTATCAATGGCCCGATCGATGACATCCTGTTCCCTATCCAGCATGGCATCCTGCATTACCCCGGTTTCGA
GGTGCTCCCCCCCTTTGTCATCTATCGTACCGGCCGAATGGATGAAACACGTTTTACCGAGATCGGTGACGCCCTGGGGC
AACGGCTGGACGACCTGGCGCATACCGCACCAATCCCTTTCCGCCGACAAAATGCGGGCGAATACGAGATCCCGGCATTA
ACCCTGAAACCCGATATTGCCCCCGGCCGGGGTGGTTTTGGTGCACATATAGAATAA

Upstream 100 bases:

>100_bases
CTGATATTATTCATGGTAATACCTGTAATATTAGAAATTCATTTCTAGTATACCTGATAAGCGCCTACCCTGACTTCCAT
CAACTAATGGAGGTCCCGCT

Downstream 100 bases:

>100_bases
CGATAAGGCCCGGTTTACCGGGCCAGTTGCAAGATTACTTCTCTAGCGGATAAATGGTGCCGGTGTTCATAATGCCGTTG
GGATCGTAGACCTCTTTCAA

Product: ribosyldihydronicotinamide dehydrogenase (quinone)

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNVLLVYAHPEPQSLNGSLKDLSVQRLKAAGHQVQVSDLYAMQWKATLDANDSRVPPLGSRFDPSLDSQHAFANGLQSQD
IELEQEKLRWADTVILQFPLWWFSMPAILKGWVERVYAYGFAYGVGEHSDSRWGDRYGEGTLAGKRAMLIVTTGGWESHY
AARGINGPIDDILFPIQHGILHYPGFEVLPPFVIYRTGRMDETRFTEIGDALGQRLDDLAHTAPIPFRRQNAGEYEIPAL
TLKPDIAPGRGGFGAHIE

Sequences:

>Translated_258_residues
MNVLLVYAHPEPQSLNGSLKDLSVQRLKAAGHQVQVSDLYAMQWKATLDANDSRVPPLGSRFDPSLDSQHAFANGLQSQD
IELEQEKLRWADTVILQFPLWWFSMPAILKGWVERVYAYGFAYGVGEHSDSRWGDRYGEGTLAGKRAMLIVTTGGWESHY
AARGINGPIDDILFPIQHGILHYPGFEVLPPFVIYRTGRMDETRFTEIGDALGQRLDDLAHTAPIPFRRQNAGEYEIPAL
TLKPDIAPGRGGFGAHIE
>Mature_258_residues
MNVLLVYAHPEPQSLNGSLKDLSVQRLKAAGHQVQVSDLYAMQWKATLDANDSRVPPLGSRFDPSLDSQHAFANGLQSQD
IELEQEKLRWADTVILQFPLWWFSMPAILKGWVERVYAYGFAYGVGEHSDSRWGDRYGEGTLAGKRAMLIVTTGGWESHY
AARGINGPIDDILFPIQHGILHYPGFEVLPPFVIYRTGRMDETRFTEIGDALGQRLDDLAHTAPIPFRRQNAGEYEIPAL
TLKPDIAPGRGGFGAHIE

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=228, Percent_Identity=42.1052631578947, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4505415, Length=229, Percent_Identity=39.7379912663755, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI70995396, Length=229, Percent_Identity=33.6244541484716, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1786231, Length=214, Percent_Identity=28.0373831775701, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787129, Length=125, Percent_Identity=32.8, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789750, Length=222, Percent_Identity=27.9279279279279, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 28814; Mature: 28814

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLLVYAHPEPQSLNGSLKDLSVQRLKAAGHQVQVSDLYAMQWKATLDANDSRVPPLGS
CCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCCCCCCCCCC
RFDPSLDSQHAFANGLQSQDIELEQEKLRWADTVILQFPLWWFSMPAILKGWVERVYAYG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
FAYGVGEHSDSRWGDRYGEGTLAGKRAMLIVTTGGWESHYAARGINGPIDDILFPIQHGI
HHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCE
LHYPGFEVLPPFVIYRTGRMDETRFTEIGDALGQRLDDLAHTAPIPFRRQNAGEYEIPAL
EECCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCEE
TLKPDIAPGRGGFGAHIE
EECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNVLLVYAHPEPQSLNGSLKDLSVQRLKAAGHQVQVSDLYAMQWKATLDANDSRVPPLGS
CCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCCCCCCCCCC
RFDPSLDSQHAFANGLQSQDIELEQEKLRWADTVILQFPLWWFSMPAILKGWVERVYAYG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
FAYGVGEHSDSRWGDRYGEGTLAGKRAMLIVTTGGWESHYAARGINGPIDDILFPIQHGI
HHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCE
LHYPGFEVLPPFVIYRTGRMDETRFTEIGDALGQRLDDLAHTAPIPFRRQNAGEYEIPAL
EECCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCEE
TLKPDIAPGRGGFGAHIE
EECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA