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The map label for this gene is rfbX [H]

Identifier: 157369849

GI number: 157369849

Start: 1760070

End: 1761308

Strand: Reverse

Name: rfbX [H]

Synonym: Spro_1606

Alternate gene names: 157369849

Gene position: 1761308-1760070 (Counterclockwise)

Preceding gene: 157369850

Following gene: 157369848

Centisome position: 32.32

GC content: 46.17

Gene sequence:

>1239_bases
ATGTCTCGCGTCGCCACTAATACCGCCTGGTTAATGATCGCTCAAATCGGGGGATTGGTTATCCCATTGATTGAGCTTCC
GGTCTTGGCACATGCGCTAGGTCAGCAGGGGTACGGGCAGGTTTTGTATGCGCTGGGCATTGCGTTAACAGCATCAGTGT
TCGTTGAGTTTGGTTTTAATTTCAGCGCAGCACGCAGTGTGGTGAAAGCACAAGGTAATCAGAAACAATTGGCGCAGCTG
CTTACCAATGTCTTGCTAGCGAAAATACTATTGAGCTTGGCTGTTGGTATCGTTGTCGCTATCTTGATTTTTTCAGGTAT
CGGGGCCACGACAATCCCGAACTACTGGTTCATCTGGATATCGTTGTTCATACTGGCATTTGGCTTTACCCCGCTTTGGT
ACTATATCGGTCTCGAGAAGCTTATTTTTCCCGCGTTGCTGGATATAGGATTACGCAGTATTGGCTTGTTGGCGACCATA
GTTTTGGTTTCCACTCCCGCCCATGCACAACGTGTGTTGGCTATCCAGGCTACGGTTGGAATAATCAATACATTGCTCCC
AACGTTGCTTATCGTTCGTACCACCGGTTTTGGTCGCATAAGCTTCACGGGAGCAACCACCGTTTTACGCGAAAGCTGGG
AACTATTTCTATATAAAGGGGCCCAAAGTATCATGGGCTCAATTGCATCTACACTCTTAGGTTTACTCGGGGGCGCGCGC
GCAGTAGGCGCTTTTGTCCCAGCAGAAAAACTGGTCAAAGCTGCGAGTGGATTTGCCGGACCGGTGTTAAATGCCGCATT
TCCTCATTTAGTACGTCTGCAACACAATTCGGCTCGTGATGCGAAAAAAATGGTTGTGCTGATTTTAATTGGCTTATTCA
TCGCAACTACACTGTTTGCACTAGTTACCCTTTGGCTCGCACCATGGATCGTCAACATGGTATTCGGGCCAGGTTATGAG
GATGCCATCGAATTATTAAGAATTTTAGTATGGGTTGTTCCTTTGCGTATCAATAATATGGCACTAGCCATACTATGGTT
TATTCCTAGTGGAAAGGAAAGAATTGCCAGCAGAGCTATGATGCTGAATATCGCTATTATTTGCCTGCTCTCTTTGTTGC
TGGTTCCTGTGTATGGCGGTTTAGGCATGACCATTGCGTTCCTAAGTGCCGAAATCGTAATGTTTACTCTATTTCTACTG
TTATTTTGTAGAAAAGCCACCAAAGCAAGCGATAAATGA

Upstream 100 bases:

>100_bases
ACTCTGACTCTGTTCCTGTTTATGGGATTATTCCACCCATTAAGTAACGAACTTACTGAATGGGGTATTATTTTGGCAGC
GCTAGCTATTGTGGTTACCC

Downstream 100 bases:

>100_bases
TACTGATTTACTTTATCGTTAAGCATGGCAAACTATTATTAACTGCTTTGCTTTATTAGTATAATGAAGGAAATGAGTTT
GAATAACTTCACATTAAGCG

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSRVATNTAWLMIAQIGGLVIPLIELPVLAHALGQQGYGQVLYALGIALTASVFVEFGFNFSAARSVVKAQGNQKQLAQL
LTNVLLAKILLSLAVGIVVAILIFSGIGATTIPNYWFIWISLFILAFGFTPLWYYIGLEKLIFPALLDIGLRSIGLLATI
VLVSTPAHAQRVLAIQATVGIINTLLPTLLIVRTTGFGRISFTGATTVLRESWELFLYKGAQSIMGSIASTLLGLLGGAR
AVGAFVPAEKLVKAASGFAGPVLNAAFPHLVRLQHNSARDAKKMVVLILIGLFIATTLFALVTLWLAPWIVNMVFGPGYE
DAIELLRILVWVVPLRINNMALAILWFIPSGKERIASRAMMLNIAIICLLSLLLVPVYGGLGMTIAFLSAEIVMFTLFLL
LFCRKATKASDK

Sequences:

>Translated_412_residues
MSRVATNTAWLMIAQIGGLVIPLIELPVLAHALGQQGYGQVLYALGIALTASVFVEFGFNFSAARSVVKAQGNQKQLAQL
LTNVLLAKILLSLAVGIVVAILIFSGIGATTIPNYWFIWISLFILAFGFTPLWYYIGLEKLIFPALLDIGLRSIGLLATI
VLVSTPAHAQRVLAIQATVGIINTLLPTLLIVRTTGFGRISFTGATTVLRESWELFLYKGAQSIMGSIASTLLGLLGGAR
AVGAFVPAEKLVKAASGFAGPVLNAAFPHLVRLQHNSARDAKKMVVLILIGLFIATTLFALVTLWLAPWIVNMVFGPGYE
DAIELLRILVWVVPLRINNMALAILWFIPSGKERIASRAMMLNIAIICLLSLLLVPVYGGLGMTIAFLSAEIVMFTLFLL
LFCRKATKASDK
>Mature_411_residues
SRVATNTAWLMIAQIGGLVIPLIELPVLAHALGQQGYGQVLYALGIALTASVFVEFGFNFSAARSVVKAQGNQKQLAQLL
TNVLLAKILLSLAVGIVVAILIFSGIGATTIPNYWFIWISLFILAFGFTPLWYYIGLEKLIFPALLDIGLRSIGLLATIV
LVSTPAHAQRVLAIQATVGIINTLLPTLLIVRTTGFGRISFTGATTVLRESWELFLYKGAQSIMGSIASTLLGLLGGARA
VGAFVPAEKLVKAASGFAGPVLNAAFPHLVRLQHNSARDAKKMVVLILIGLFIATTLFALVTLWLAPWIVNMVFGPGYED
AIELLRILVWVVPLRINNMALAILWFIPSGKERIASRAMMLNIAIICLLSLLLVPVYGGLGMTIAFLSAEIVMFTLFLLL
FCRKATKASDK

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=333, Percent_Identity=25.5255255255255, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 44403; Mature: 44272

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVATNTAWLMIAQIGGLVIPLIELPVLAHALGQQGYGQVLYALGIALTASVFVEFGFN
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
FSAARSVVKAQGNQKQLAQLLTNVLLAKILLSLAVGIVVAILIFSGIGATTIPNYWFIWI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SLFILAFGFTPLWYYIGLEKLIFPALLDIGLRSIGLLATIVLVSTPAHAQRVLAIQATVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IINTLLPTLLIVRTTGFGRISFTGATTVLRESWELFLYKGAQSIMGSIASTLLGLLGGAR
HHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVGAFVPAEKLVKAASGFAGPVLNAAFPHLVRLQHNSARDAKKMVVLILIGLFIATTLFA
HHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LVTLWLAPWIVNMVFGPGYEDAIELLRILVWVVPLRINNMALAILWFIPSGKERIASRAM
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHH
MLNIAIICLLSLLLVPVYGGLGMTIAFLSAEIVMFTLFLLLFCRKATKASDK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRVATNTAWLMIAQIGGLVIPLIELPVLAHALGQQGYGQVLYALGIALTASVFVEFGFN
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
FSAARSVVKAQGNQKQLAQLLTNVLLAKILLSLAVGIVVAILIFSGIGATTIPNYWFIWI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SLFILAFGFTPLWYYIGLEKLIFPALLDIGLRSIGLLATIVLVSTPAHAQRVLAIQATVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IINTLLPTLLIVRTTGFGRISFTGATTVLRESWELFLYKGAQSIMGSIASTLLGLLGGAR
HHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVGAFVPAEKLVKAASGFAGPVLNAAFPHLVRLQHNSARDAKKMVVLILIGLFIATTLFA
HHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LVTLWLAPWIVNMVFGPGYEDAIELLRILVWVVPLRINNMALAILWFIPSGKERIASRAM
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHH
MLNIAIICLLSLLLVPVYGGLGMTIAFLSAEIVMFTLFLLLFCRKATKASDK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7692219 [H]