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The map label for this gene is cynT [H]

Identifier: 157369777

GI number: 157369777

Start: 1671188

End: 1671841

Strand: Reverse

Name: cynT [H]

Synonym: Spro_1534

Alternate gene names: 157369777

Gene position: 1671841-1671188 (Counterclockwise)

Preceding gene: 157369782

Following gene: 157369776

Centisome position: 30.68

GC content: 57.34

Gene sequence:

>654_bases
ATGAAAGAGGTTATTGAAGGCTTTTTGAAGTTCCAGCGTGAGGCGTTTGTCGAACGCACGGCACTGTTCCAGCGGCTGGC
GACCCAGCAGAGCCCACGCACCCTGTTTATCTCCTGTTCCGACAGCCGTCTGGTGCCGGAGCTGATTACCCAGCGCGAAC
CGGGCGACCTGTTTGTGATCCGCAATGCCGGCAATATCGTGCCTTCGTTCGGCCCGGAGCCGGGTGGCGTCTCCGCATCA
GTAGAATATGCCGTCTCTGCACTCGGCGTTGAAGATATCGTGATCTGCGGACATTCCGACTGTGGCGCAATGACTGCCAT
CGCCACCTGCCAGTGTCTGCAACATATGCCGACCGTGGCTAACTGGCTGCGCTATGCCGACTCCGCCAAGGTGGTCAATC
AGGCTTATCAGCATGCATCCGAAAACGAGAAAGTCAGTTCGATGGTGCGTGAGAACGTCATCGCACAGCTGAACAATATC
AAGACCCACCCGTCGGTGGCACTGGCGCTTGAACAAGGCCGGTTGAAGCTGCACGGTTGGGTGTATGACATCGCCAGCGG
CGGTATTGAAGCGCTGGATGGCGAAACCCGCCGTTTTATTCCGCTGGCAACCAATCCTGAGGTCACGGCAACCCCTGCCG
TATCTCGCTTTTAA

Upstream 100 bases:

>100_bases
TAGTAATGCCTATAGCTATCATTATAAATGGGTCTTAGACCCTATAGCCAGAGCGGCGCAAGATACGTCCATCGCTTAAC
AGCACCCAGATGGAGGCAGT

Downstream 100 bases:

>100_bases
TTTCATTTATAAGGAAACAATATGACTCAGTCACTGCACTACTCATCCCCGCGCGAAACCCTGACTGACACCATTATGAT
GGCCAAGATCCGCAAAAACC

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKEVIEGFLKFQREAFVERTALFQRLATQQSPRTLFISCSDSRLVPELITQREPGDLFVIRNAGNIVPSFGPEPGGVSAS
VEYAVSALGVEDIVICGHSDCGAMTAIATCQCLQHMPTVANWLRYADSAKVVNQAYQHASENEKVSSMVRENVIAQLNNI
KTHPSVALALEQGRLKLHGWVYDIASGGIEALDGETRRFIPLATNPEVTATPAVSRF

Sequences:

>Translated_217_residues
MKEVIEGFLKFQREAFVERTALFQRLATQQSPRTLFISCSDSRLVPELITQREPGDLFVIRNAGNIVPSFGPEPGGVSAS
VEYAVSALGVEDIVICGHSDCGAMTAIATCQCLQHMPTVANWLRYADSAKVVNQAYQHASENEKVSSMVRENVIAQLNNI
KTHPSVALALEQGRLKLHGWVYDIASGGIEALDGETRRFIPLATNPEVTATPAVSRF
>Mature_217_residues
MKEVIEGFLKFQREAFVERTALFQRLATQQSPRTLFISCSDSRLVPELITQREPGDLFVIRNAGNIVPSFGPEPGGVSAS
VEYAVSALGVEDIVICGHSDCGAMTAIATCQCLQHMPTVANWLRYADSAKVVNQAYQHASENEKVSSMVRENVIAQLNNI
KTHPSVALALEQGRLKLHGWVYDIASGGIEALDGETRRFIPLATNPEVTATPAVSRF

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=212, Percent_Identity=76.8867924528302, Blast_Score=353, Evalue=7e-99,
Organism=Escherichia coli, GI1786318, Length=183, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25147564, Length=237, Percent_Identity=29.535864978903, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115532990, Length=240, Percent_Identity=25.4166666666667, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324292, Length=181, Percent_Identity=32.0441988950276, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24645213, Length=257, Percent_Identity=23.7354085603113, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23687; Mature: 23687

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVIEGFLKFQREAFVERTALFQRLATQQSPRTLFISCSDSRLVPELITQREPGDLFVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEE
RNAGNIVPSFGPEPGGVSASVEYAVSALGVEDIVICGHSDCGAMTAIATCQCLQHMPTVA
ECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHH
NWLRYADSAKVVNQAYQHASENEKVSSMVRENVIAQLNNIKTHPSVALALEQGRLKLHGW
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEHE
VYDIASGGIEALDGETRRFIPLATNPEVTATPAVSRF
EEEHHCCCEECCCCCCEEEEEECCCCCCEECCCCCCC
>Mature Secondary Structure
MKEVIEGFLKFQREAFVERTALFQRLATQQSPRTLFISCSDSRLVPELITQREPGDLFVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEE
RNAGNIVPSFGPEPGGVSASVEYAVSALGVEDIVICGHSDCGAMTAIATCQCLQHMPTVA
ECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHH
NWLRYADSAKVVNQAYQHASENEKVSSMVRENVIAQLNNIKTHPSVALALEQGRLKLHGW
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEHE
VYDIASGGIEALDGETRRFIPLATNPEVTATPAVSRF
EEEHHCCCEECCCCCCEEEEEECCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]