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The map label for this gene is rob [H]

Identifier: 157369766

GI number: 157369766

Start: 1661567

End: 1662025

Strand: Reverse

Name: rob [H]

Synonym: Spro_1523

Alternate gene names: 157369766

Gene position: 1662025-1661567 (Counterclockwise)

Preceding gene: 157369767

Following gene: 157369761

Centisome position: 30.5

GC content: 56.64

Gene sequence:

>459_bases
ATGGATCGCGTCAATATCATTCACGATTTGCTGGACTGGATTGAAAATCATCTGGATCAACCCTTGCTGTTAGACAACGT
GGCAGCCAAGTCAGGCTACTCCAAGTGGCACTTGCAAAGGATGTTCCGCAGCACCACCGGCCACGCGCTGGGCAGCTATA
TCCGCGAGCGTCGTCTTTCGCAGGCGGCACAGGCGCTGCGTTCAAGCCCTCGTCCTATTCTCGACATCGCCCTGCAGTTC
CATTTTGATTCCCAACCGTCATTTTCACGCGCGTTCAAAAAACAGTTCGGCAAAACGCCGGCGGTGTATCGCCGCACGAC
TCGCTGGGATGTGGCAGAAATGCGACCGCAGGCTATCGAACCGCTGAACGGACATCGCCATGAGTCGCCGGGCGTGCATC
TGTGGTATGCGGGTAAATCTCTGGACGGGGTCTGCACCAACTGGGTTGGCCAGCACTGA

Upstream 100 bases:

>100_bases
CTTGCGTAACCCGGTGTTGCGGCCTATTATCAAAGCACAAGCTTGGATAATTACTCTTACCTACACTGACCTGAATGTCG
TACTGCGGGAGAACTCAAAC

Downstream 100 bases:

>100_bases
GATAAATAGGCATTAAAAAAGGGGTTCAGCCTGCGCTGAACCCCTTTTTTTTACTGCCAGCTCTTACTTAAAGGTGACGT
CGATATTTGCCGATGCGGTG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MDRVNIIHDLLDWIENHLDQPLLLDNVAAKSGYSKWHLQRMFRSTTGHALGSYIRERRLSQAAQALRSSPRPILDIALQF
HFDSQPSFSRAFKKQFGKTPAVYRRTTRWDVAEMRPQAIEPLNGHRHESPGVHLWYAGKSLDGVCTNWVGQH

Sequences:

>Translated_152_residues
MDRVNIIHDLLDWIENHLDQPLLLDNVAAKSGYSKWHLQRMFRSTTGHALGSYIRERRLSQAAQALRSSPRPILDIALQF
HFDSQPSFSRAFKKQFGKTPAVYRRTTRWDVAEMRPQAIEPLNGHRHESPGVHLWYAGKSLDGVCTNWVGQH
>Mature_152_residues
MDRVNIIHDLLDWIENHLDQPLLLDNVAAKSGYSKWHLQRMFRSTTGHALGSYIRERRLSQAAQALRSSPRPILDIALQF
HFDSQPSFSRAFKKQFGKTPAVYRRTTRWDVAEMRPQAIEPLNGHRHESPGVHLWYAGKSLDGVCTNWVGQH

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=116, Percent_Identity=68.1034482758621, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=56.7307692307692, Blast_Score=126, Evalue=6e-31,
Organism=Escherichia coli, GI87081928, Length=100, Percent_Identity=52, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 17555; Mature: 17555

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVNIIHDLLDWIENHLDQPLLLDNVAAKSGYSKWHLQRMFRSTTGHALGSYIRERRLS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAAQALRSSPRPILDIALQFHFDSQPSFSRAFKKQFGKTPAVYRRTTRWDVAEMRPQAIE
HHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCHHHHCHHHCC
PLNGHRHESPGVHLWYAGKSLDGVCTNWVGQH
CCCCCCCCCCCEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MDRVNIIHDLLDWIENHLDQPLLLDNVAAKSGYSKWHLQRMFRSTTGHALGSYIRERRLS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAAQALRSSPRPILDIALQFHFDSQPSFSRAFKKQFGKTPAVYRRTTRWDVAEMRPQAIE
HHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCHHHHCHHHCC
PLNGHRHESPGVHLWYAGKSLDGVCTNWVGQH
CCCCCCCCCCCEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]