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The map label for this gene is betT [H]

Identifier: 157369755

GI number: 157369755

Start: 1646501

End: 1648540

Strand: Reverse

Name: betT [H]

Synonym: Spro_1512

Alternate gene names: 157369755

Gene position: 1648540-1646501 (Counterclockwise)

Preceding gene: 157369759

Following gene: 157369751

Centisome position: 30.25

GC content: 55.98

Gene sequence:

>2040_bases
ATGACAACACGCGAAACCTCCTCAAAACCGCAAAAGGATGGCCTTAATCCTGTGGTTTTCTTTACCTCGGCAGGGCTGAT
TTTGGCCTTCTCGCTGATGACCATCTTTTTTACCGATTTCTCCGGGCGATGGATCACCCGCACCCTGAATTGGGTTTCAA
CCACCTTCGGCTGGTACTATCTGCTGGCCGCCACCCTGTACATCGTATTTGTGGTGTTTATCGCCGCTTCGCGCTTTGGT
TCTATCAAGCTCGGGCCTGAACAATCAAAACCCGAATTCAGCCTGATGAGCTGGGCGGCGATGCTGTTCGCCGCCGGTAT
CGGCATCGATCTGATGTTCTTCTCGGTCGCCGAACCGGTGACACAATATATGATGCCGCCGGAAGGCCAGGGCCAGACGC
TGGAAGCCGCACGCCAGGCAATGGTCTGGACGCTGTTCCACTACGGCCTGACCGGCTGGTCGATGTATGCGTTGATGGGT
ATCGCCCTCGGCTATTTCAGCTACCGCTATAACCTGCCGCTGACCATCCGCTCTGCCCTGTACCCGATTTTCGGTAAACG
CATCAATGGCCCGATTGGCCACAGCGTAGATATCGCCGCGGTGCTGGGTACCATCTTCGGTATCGCCACCACGCTCGGTA
TTGGCGTAGTGCAGTTGAACTACGGGCTGAAAGTGCTGTTCCACATACCGGAAAACCTGACGGTTCAGGCCGCGTTGATC
CTGCTTTCGGTGATCATGGCCACCATTTCGGTGACTTCCGGCGTCAATAAGGGCATCCGTATTCTGTCCGAGCTCAACGT
GCTGTTGGCACTGGGGCTGATTTTATTCGTGCTGTTCTTCGGCGATACCGAGTTCCTGCTCAATGCGCTGGTGCTTAACG
TCGGTGATTACGTCAACCGCTTTATGGGCATGACGCTCAACAGCTTCGCCTTCGATCGCCCGGTGGAATGGATGAACAAC
TGGACCCTGTTCTTCTGGGCATGGTGGGTGGCCTGGGCGCCGTTTGTCGGCCTGTTCCTGGCGCGAATTTCACGTGGGCG
CACCATTCGCCAGTTTGTGGTCGGCACGCTGATTATTCCGTTCGTGTTTACCCTGCTGTGGTTGTCAATCTTCGGTAACA
GCGCGCTGTACCAGATTATTCACGGCAACGCCGCCTTCGCGCAGGAAGTGATGCAGTACCCGGAACGGGGGTTCTACAGC
CTGCTGGCGCAGTATCCGGGCTTTACCTTCAGCGCTTCGGTGGCCACTATCACTGGGCTGCTGTTCTACGTCACCTCAGC
GGATTCAGGCTCGCTGGTGCTGGGTAACTTCACCTCACGCCTGAGTGATATCAATAATGACGCCCCCAACTGGCTGCGTA
TTTTCTGGTCGGTGGCGATTGGTCTGCTGACCATAGGTATGTTGATGACCGACGGCGTGCCTGCCCTGCAGAAAACCACG
GTGATCATGGGCCTGCCGTTCAGTTTTGTGATCTTCTTCGTGATGGCCGGGTTGTATAAATCGCTACGGGTGGAGGACTA
CCGCAAGGCCAGCGCGCTGAACACCAATGCGCCCATGCCGGTGTCCAGCAATGACGTGCTGAACTGGAAACAGCGCCTGT
CGCGGGTAATGAATTATCCGGGCAGCCAATATACACAGAAAATGATGGATACCCGCTGTCGGCCGGCGATGCAGGAAGTG
GCGCGTGAGCTGGAATTACGCGGCGCCAAAGTGGAGTTCAGTGAAGTGCCGCCAACCGAAGATGAGCGTTTAAACCATCT
GGAGTTGCTGGTGCATTTAGGCGAGGAGCAGAACTTTATCTATCAGATCTGGCCGCAGCGTTACTCGGTGCCGGGCTTTA
CCTATCGCGCCCGCTCCGGCAAGTCGCACTACTACCGGCTGGAAACCTTCCTGATGGAAGGCACCCAGGGCAATGACCTG
ATGGACTACAGCAAGGAACAGGTGATCGGCGATATCCTCGATCAGTACGAAAAACACCTGAACTTTGTGCACATCCACCG
CGAGGCACCGGGTAACACCCTGACCTTCCCGGATATGTAA

Upstream 100 bases:

>100_bases
CGGCGTTCTTATCCGCACTGTTTCCGGGCGACACCCGCGTGTCGTTCAGGCTGGTGTGTGGGCACGATTTTACTCAGTTG
TTAAAAAACAGAGGAAGACG

Downstream 100 bases:

>100_bases
GCGCTAAACCGCTGTTAAACACCAAAGGCCACATCGCGTGGCCTTTGGTTTTCAGGGTAGCCGGAGCCTGCGCCACTTAT
TTCCCCATGGCGTCCTTTTT

Product: choline transport protein BetT

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MTTRETSSKPQKDGLNPVVFFTSAGLILAFSLMTIFFTDFSGRWITRTLNWVSTTFGWYYLLAATLYIVFVVFIAASRFG
SIKLGPEQSKPEFSLMSWAAMLFAAGIGIDLMFFSVAEPVTQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMG
IALGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVLGTIFGIATTLGIGVVQLNYGLKVLFHIPENLTVQAALI
LLSVIMATISVTSGVNKGIRILSELNVLLALGLILFVLFFGDTEFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNN
WTLFFWAWWVAWAPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLSIFGNSALYQIIHGNAAFAQEVMQYPERGFYS
LLAQYPGFTFSASVATITGLLFYVTSADSGSLVLGNFTSRLSDINNDAPNWLRIFWSVAIGLLTIGMLMTDGVPALQKTT
VIMGLPFSFVIFFVMAGLYKSLRVEDYRKASALNTNAPMPVSSNDVLNWKQRLSRVMNYPGSQYTQKMMDTRCRPAMQEV
ARELELRGAKVEFSEVPPTEDERLNHLELLVHLGEEQNFIYQIWPQRYSVPGFTYRARSGKSHYYRLETFLMEGTQGNDL
MDYSKEQVIGDILDQYEKHLNFVHIHREAPGNTLTFPDM

Sequences:

>Translated_679_residues
MTTRETSSKPQKDGLNPVVFFTSAGLILAFSLMTIFFTDFSGRWITRTLNWVSTTFGWYYLLAATLYIVFVVFIAASRFG
SIKLGPEQSKPEFSLMSWAAMLFAAGIGIDLMFFSVAEPVTQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMG
IALGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVLGTIFGIATTLGIGVVQLNYGLKVLFHIPENLTVQAALI
LLSVIMATISVTSGVNKGIRILSELNVLLALGLILFVLFFGDTEFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNN
WTLFFWAWWVAWAPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLSIFGNSALYQIIHGNAAFAQEVMQYPERGFYS
LLAQYPGFTFSASVATITGLLFYVTSADSGSLVLGNFTSRLSDINNDAPNWLRIFWSVAIGLLTIGMLMTDGVPALQKTT
VIMGLPFSFVIFFVMAGLYKSLRVEDYRKASALNTNAPMPVSSNDVLNWKQRLSRVMNYPGSQYTQKMMDTRCRPAMQEV
ARELELRGAKVEFSEVPPTEDERLNHLELLVHLGEEQNFIYQIWPQRYSVPGFTYRARSGKSHYYRLETFLMEGTQGNDL
MDYSKEQVIGDILDQYEKHLNFVHIHREAPGNTLTFPDM
>Mature_678_residues
TTRETSSKPQKDGLNPVVFFTSAGLILAFSLMTIFFTDFSGRWITRTLNWVSTTFGWYYLLAATLYIVFVVFIAASRFGS
IKLGPEQSKPEFSLMSWAAMLFAAGIGIDLMFFSVAEPVTQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMGI
ALGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVLGTIFGIATTLGIGVVQLNYGLKVLFHIPENLTVQAALIL
LSVIMATISVTSGVNKGIRILSELNVLLALGLILFVLFFGDTEFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNW
TLFFWAWWVAWAPFVGLFLARISRGRTIRQFVVGTLIIPFVFTLLWLSIFGNSALYQIIHGNAAFAQEVMQYPERGFYSL
LAQYPGFTFSASVATITGLLFYVTSADSGSLVLGNFTSRLSDINNDAPNWLRIFWSVAIGLLTIGMLMTDGVPALQKTTV
IMGLPFSFVIFFVMAGLYKSLRVEDYRKASALNTNAPMPVSSNDVLNWKQRLSRVMNYPGSQYTQKMMDTRCRPAMQEVA
RELELRGAKVEFSEVPPTEDERLNHLELLVHLGEEQNFIYQIWPQRYSVPGFTYRARSGKSHYYRLETFLMEGTQGNDLM
DYSKEQVIGDILDQYEKHLNFVHIHREAPGNTLTFPDM

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=674, Percent_Identity=82.9376854599407, Blast_Score=1169, Evalue=0.0,
Organism=Escherichia coli, GI1788102, Length=482, Percent_Identity=28.6307053941909, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1786224, Length=509, Percent_Identity=27.3084479371316, Blast_Score=161, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 76536; Mature: 76405

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRETSSKPQKDGLNPVVFFTSAGLILAFSLMTIFFTDFSGRWITRTLNWVSTTFGWYY
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LLAATLYIVFVVFIAASRFGSIKLGPEQSKPEFSLMSWAAMLFAAGIGIDLMFFSVAEPV
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMGIALGYFSYRYNLPLTIRSAL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCEEHHHHH
YPIFGKRINGPIGHSVDIAAVLGTIFGIATTLGIGVVQLNYGLKVLFHIPENLTVQAALI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHHHHH
LLSVIMATISVTSGVNKGIRILSELNVLLALGLILFVLFFGDTEFLLNALVLNVGDYVNR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWAPFVGLFLARISRGRTIRQFVVGTLIIP
HHCCHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVFTLLWLSIFGNSALYQIIHGNAAFAQEVMQYPERGFYSLLAQYPGFTFSASVATITGL
HHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEHHHHHHHHH
LFYVTSADSGSLVLGNFTSRLSDINNDAPNWLRIFWSVAIGLLTIGMLMTDGVPALQKTT
HHHEECCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCE
VIMGLPFSFVIFFVMAGLYKSLRVEDYRKASALNTNAPMPVSSNDVLNWKQRLSRVMNYP
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
GSQYTQKMMDTRCRPAMQEVARELELRGAKVEFSEVPPTEDERLNHLELLVHLGEEQNFI
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEE
YQIWPQRYSVPGFTYRARSGKSHYYRLETFLMEGTQGNDLMDYSKEQVIGDILDQYEKHL
EEECCCCCCCCCCEEECCCCCCHHEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NFVHIHREAPGNTLTFPDM
CEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TTRETSSKPQKDGLNPVVFFTSAGLILAFSLMTIFFTDFSGRWITRTLNWVSTTFGWYY
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LLAATLYIVFVVFIAASRFGSIKLGPEQSKPEFSLMSWAAMLFAAGIGIDLMFFSVAEPV
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMGIALGYFSYRYNLPLTIRSAL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCEEHHHHH
YPIFGKRINGPIGHSVDIAAVLGTIFGIATTLGIGVVQLNYGLKVLFHIPENLTVQAALI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHHHHH
LLSVIMATISVTSGVNKGIRILSELNVLLALGLILFVLFFGDTEFLLNALVLNVGDYVNR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWAPFVGLFLARISRGRTIRQFVVGTLIIP
HHCCHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVFTLLWLSIFGNSALYQIIHGNAAFAQEVMQYPERGFYSLLAQYPGFTFSASVATITGL
HHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEHHHHHHHHH
LFYVTSADSGSLVLGNFTSRLSDINNDAPNWLRIFWSVAIGLLTIGMLMTDGVPALQKTT
HHHEECCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCE
VIMGLPFSFVIFFVMAGLYKSLRVEDYRKASALNTNAPMPVSSNDVLNWKQRLSRVMNYP
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
GSQYTQKMMDTRCRPAMQEVARELELRGAKVEFSEVPPTEDERLNHLELLVHLGEEQNFI
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEE
YQIWPQRYSVPGFTYRARSGKSHYYRLETFLMEGTQGNDLMDYSKEQVIGDILDQYEKHL
EEECCCCCCCCCCEEECCCCCCHHEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NFVHIHREAPGNTLTFPDM
CEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]