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The map label for this gene is ramA [H]

Identifier: 157369728

GI number: 157369728

Start: 1619786

End: 1620769

Strand: Reverse

Name: ramA [H]

Synonym: Spro_1485

Alternate gene names: 157369728

Gene position: 1620769-1619786 (Counterclockwise)

Preceding gene: 157369730

Following gene: 157369726

Centisome position: 29.75

GC content: 59.76

Gene sequence:

>984_bases
ATGACAAACTTTCTGCGTGCCGCGACGGTGCAGTTCCAGCATCGCGCCAACGATAAAAATTACAACCTGTCGGTGATCGA
GGGCTTTATCACTCGCGCCGCCGCCGAACAAATCCAGGTGCTGACCTTCCCGGAGATGTGTATTACCGGCTATTGGCACG
TGCGACACCTCAGCGCCGGCGAAATTGGCGCGCTGGCGGAACCCATTGCCACCAGCCCTTCGCTGGCGCGTATTCGTCCG
CTGGCGGAGCGCTACAACATGGCGATCGGTGTTGGGCTCATCGAACAGGGTGAAGACGGCAAATATTACAATGCCTACGC
GGTTTGCCTGCCCGACGGCAAGCTTCACGTACACCGAAAACTGCATGCCTTTGAGCACCCGGCGATAGCCAGCGGCGACA
GCTTCACCGTGTTTGACACCCCATGGGGGGTTCGGATGGGAGTGTTGATCTGCTGGGACAATAACCTGGTAGAGAATGTA
CGTGCGACCGCTTTGCTGGGGGCTGAAGTCCTGCTGGCTCCGCATCAAACGGGCGGCACCCATTCCCGTAGCCCGCATGG
TATGAAGCCAATACCGTTGGAAGTCTGGCAGCAGCGGCAGACAAATCCGGCGGCGATAGAGGCAGCATTTCGTGGCGAAC
ATGGCCGCGGCTGGCTGTTGCGCTGGTTACCGGCTCGCGCTCATGACAATGGCCTTTTTGTTCTGTTCAGCAATGGTGTG
GGGCGCGATGACGACGAGGTCCGCACCGGCAATGCGATGATCCTCGACCCGTATGGCCGTATTTTGGCGGAAACCTGGGC
GGCGGAAAATCAGATGGTCACGGCGGAACTGGATCTGTCGCTGATCCCGCTCAGTACCGGACGCCGCTGGATCCACGGCC
GTCGGCCCGCGCTGTACGGTATTCTCACCCAGCCACAGGGCTATGAGCGAGATGCCCGCAGCGCACGCTTTTCGTCAGAG
CCGGCACAGAAACTGGGTAAGTAG

Upstream 100 bases:

>100_bases
TATCCATTCCGTTTGGTTATCAAATTAAGAATTTAATTGATTATACAGTAATCAATAGCTTTGCTGTAATCAACGCCAGA
CAACGACCAAAGGTAACCTT

Downstream 100 bases:

>100_bases
CCACCCGCGCCGAATGACAGGCAAAAAAAATCCGCCCGAGGGCGGATTTTTATCTGATGCGGCCAATTGCGACACACGCT
CACACCCGTAGGCGTGGAGA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTNFLRAATVQFQHRANDKNYNLSVIEGFITRAAAEQIQVLTFPEMCITGYWHVRHLSAGEIGALAEPIATSPSLARIRP
LAERYNMAIGVGLIEQGEDGKYYNAYAVCLPDGKLHVHRKLHAFEHPAIASGDSFTVFDTPWGVRMGVLICWDNNLVENV
RATALLGAEVLLAPHQTGGTHSRSPHGMKPIPLEVWQQRQTNPAAIEAAFRGEHGRGWLLRWLPARAHDNGLFVLFSNGV
GRDDDEVRTGNAMILDPYGRILAETWAAENQMVTAELDLSLIPLSTGRRWIHGRRPALYGILTQPQGYERDARSARFSSE
PAQKLGK

Sequences:

>Translated_327_residues
MTNFLRAATVQFQHRANDKNYNLSVIEGFITRAAAEQIQVLTFPEMCITGYWHVRHLSAGEIGALAEPIATSPSLARIRP
LAERYNMAIGVGLIEQGEDGKYYNAYAVCLPDGKLHVHRKLHAFEHPAIASGDSFTVFDTPWGVRMGVLICWDNNLVENV
RATALLGAEVLLAPHQTGGTHSRSPHGMKPIPLEVWQQRQTNPAAIEAAFRGEHGRGWLLRWLPARAHDNGLFVLFSNGV
GRDDDEVRTGNAMILDPYGRILAETWAAENQMVTAELDLSLIPLSTGRRWIHGRRPALYGILTQPQGYERDARSARFSSE
PAQKLGK
>Mature_326_residues
TNFLRAATVQFQHRANDKNYNLSVIEGFITRAAAEQIQVLTFPEMCITGYWHVRHLSAGEIGALAEPIATSPSLARIRPL
AERYNMAIGVGLIEQGEDGKYYNAYAVCLPDGKLHVHRKLHAFEHPAIASGDSFTVFDTPWGVRMGVLICWDNNLVENVR
ATALLGAEVLLAPHQTGGTHSRSPHGMKPIPLEVWQQRQTNPAAIEAAFRGEHGRGWLLRWLPARAHDNGLFVLFSNGVG
RDDDEVRTGNAMILDPYGRILAETWAAENQMVTAELDLSLIPLSTGRRWIHGRRPALYGILTQPQGYERDARSARFSSEP
AQKLGK

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 36319; Mature: 36188

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFLRAATVQFQHRANDKNYNLSVIEGFITRAAAEQIQVLTFPEMCITGYWHVRHLSAG
CCCCHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHCCEEEEEEECCC
EIGALAEPIATSPSLARIRPLAERYNMAIGVGLIEQGEDGKYYNAYAVCLPDGKLHVHRK
CCCHHHCCCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEEEEEEECCCCEEEEEH
LHAFEHPAIASGDSFTVFDTPWGVRMGVLICWDNNLVENVRATALLGAEVLLAPHQTGGT
HHHHCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHEECCEEEEECCCCCCC
HSRSPHGMKPIPLEVWQQRQTNPAAIEAAFRGEHGRGWLLRWLPARAHDNGLFVLFSNGV
CCCCCCCCCCCCHHHHHCCCCCHHEEEEEECCCCCCCEEEEEEECEECCCCEEEEEECCC
GRDDDEVRTGNAMILDPYGRILAETWAAENQMVTAELDLSLIPLSTGRRWIHGRRPALYG
CCCCCCEECCCEEEECCHHHHHHHHHCCCCCEEEEEECEEEEEECCCCCEECCCCCEEEE
ILTQPQGYERDARSARFSSEPAQKLGK
EECCCCCCCCHHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
TNFLRAATVQFQHRANDKNYNLSVIEGFITRAAAEQIQVLTFPEMCITGYWHVRHLSAG
CCCHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHCCEEEEEEECCC
EIGALAEPIATSPSLARIRPLAERYNMAIGVGLIEQGEDGKYYNAYAVCLPDGKLHVHRK
CCCHHHCCCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEEEEEEECCCCEEEEEH
LHAFEHPAIASGDSFTVFDTPWGVRMGVLICWDNNLVENVRATALLGAEVLLAPHQTGGT
HHHHCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHEECCEEEEECCCCCCC
HSRSPHGMKPIPLEVWQQRQTNPAAIEAAFRGEHGRGWLLRWLPARAHDNGLFVLFSNGV
CCCCCCCCCCCCHHHHHCCCCCHHEEEEEECCCCCCCEEEEEEECEECCCCEEEEEECCC
GRDDDEVRTGNAMILDPYGRILAETWAAENQMVTAELDLSLIPLSTGRRWIHGRRPALYG
CCCCCCEECCCEEEECCHHHHHHHHHCCCCCEEEEEECEEEEEECCCCCEECCCCCEEEE
ILTQPQGYERDARSARFSSEPAQKLGK
EECCCCCCCCHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA