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The map label for this gene is miaB

Identifier: 157369463

GI number: 157369463

Start: 1336426

End: 1337850

Strand: Reverse

Name: miaB

Synonym: Spro_1220

Alternate gene names: 157369463

Gene position: 1337850-1336426 (Counterclockwise)

Preceding gene: 157369465

Following gene: 157369462

Centisome position: 24.55

GC content: 56.91

Gene sequence:

>1425_bases
ATGACCAAAAAACTACATATCAAAACCTGGGGCTGCCAGATGAATGAATATGATTCATCAAAAATGGCGGACTTACTGGG
AAGCACGCACGGTTATCAATGGACCGACAACGCTGAAGAAGCGGACGTGCTGCTGCTCAATACCTGCTCGATCCGCGAGA
AAGCGCAGGAAAAAGTTTTCGCCATGTTGGGCCGCTGGAGGCTGCTGAAAGAAAAGAACCCGGCGCTGATTATCGGCGTG
GGGGGCTGCGTGGCCTCGCAGGAAGGTGAACACATCCGTAGCCGTGCGCCCTGTGTCGACGTGGTGTTTGGGCCGCAAAC
CCTGCACCGCCTGCCGGAAATGCTCAATCACGTGCAGGGCACCCGCAGCCCAATCGTCGATATCAGCTTCCCGGAGATCG
AAAAGTTCGACCGCTTGCCGGAACCCCGTGCTGAAGGCCCGACCGCCTTTGTCTCCATCATGGAAGGCTGCAACAAATAC
TGTACCTTCTGCGTGGTGCCTTATACCCGCGGAGAAGAAGTCAGCCGCCCAAGCGACGACGTACTGTTTGAAGTCGCCCA
ACTGGCGGCGCAGGGCGTGCGTGAAGTCAACCTGCTCGGCCAGAACGTCAATGCCTACCGCGGGGCAACCTACGACGGCG
GCATCTGCTCCTTTGCCGAACTGTTGCGCCTGGTGGCGGCCATTGACGGCATCGACCGCATCCGCTACACCACCAGCCAT
CCGATTGAATTCACCGACGATATTATTGCGGTATACGAAGACACGCCGGAGTTGGTCAGCTTCCTGCATCTGCCGGTACA
GAGCGGTTCAGACCGCATACTGACCATGATGAAGCGCGCGCACACCGCACTGGAATACAAAGCGATCATTCGCAAACTGC
GCAAGGCGCGGCCGGACATTCAGCTCAGCTCTGATTTTATTATCGGCTTCCCAGGGGAAACCCAGGCCGACTTCGAACAG
ACCATGAACCTGATTGCCGAGATCAACTTCGACACCAGCTTCAGCTTTATCTACTCGTCACGCCCAGGTACCCCGGCGGC
CGATATGGTGGACGACGTCAGCGAAGACGAAAAGAAACAGCGCCTGTATATCCTGCAGGATCGCATCAACCAGCAGGTGC
TGCAGTTCAGCCGTCGAATGCTCGGTACCGTTCAGCGCATTTTGGTGGAAGGCACCTCACGTAAAAGCGTGATGGAGCTG
GCGGGGCGTACCGCCTGCAACCGCATGGTAAACTTTGAAGGCACGCCGGATATGATTGGCCAGTTCGTGGATGTGGAAAT
CACCGAAGTTTTGACCAACTCACTGCGTGGCACAGTGGTGCGTACCGAACAGCAGATGGATCTGCGCGTACATGAATCCC
CGCAGTCGGTGATCGCCCGTACCCGCAAAGAGAATGCGCTGGGTGTCGGCATTTACCAGCCCTGA

Upstream 100 bases:

>100_bases
CGTTTTCCCCGCGCTGGTCACAACGGCGTCAAATGTTTACAATAGCGCCCTGCATGTCGCGTATCGCAACTTTCGCTCCG
CACTGAGTAATGGCAAGTCA

Downstream 100 bases:

>100_bases
CGCTGTTTTAGTCTGGCGCTAACCGTGGCGGCCGCGCTTCCCGCCGCCACGATAACGATGATCCTTATTATTTCTCTGTC
GGGCCATGCGCCCGGCAATT

Product: tRNA-i(6)A37 thiotransferase protein MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLGSTHGYQWTDNAEEADVLLLNTCSIREKAQEKVFAMLGRWRLLKEKNPALIIGV
GGCVASQEGEHIRSRAPCVDVVFGPQTLHRLPEMLNHVQGTRSPIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPSDDVLFEVAQLAAQGVREVNLLGQNVNAYRGATYDGGICSFAELLRLVAAIDGIDRIRYTTSH
PIEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRKARPDIQLSSDFIIGFPGETQADFEQ
TMNLIAEINFDTSFSFIYSSRPGTPAADMVDDVSEDEKKQRLYILQDRINQQVLQFSRRMLGTVQRILVEGTSRKSVMEL
AGRTACNRMVNFEGTPDMIGQFVDVEITEVLTNSLRGTVVRTEQQMDLRVHESPQSVIARTRKENALGVGIYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLGSTHGYQWTDNAEEADVLLLNTCSIREKAQEKVFAMLGRWRLLKEKNPALIIGV
GGCVASQEGEHIRSRAPCVDVVFGPQTLHRLPEMLNHVQGTRSPIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPSDDVLFEVAQLAAQGVREVNLLGQNVNAYRGATYDGGICSFAELLRLVAAIDGIDRIRYTTSH
PIEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRKARPDIQLSSDFIIGFPGETQADFEQ
TMNLIAEINFDTSFSFIYSSRPGTPAADMVDDVSEDEKKQRLYILQDRINQQVLQFSRRMLGTVQRILVEGTSRKSVMEL
AGRTACNRMVNFEGTPDMIGQFVDVEITEVLTNSLRGTVVRTEQQMDLRVHESPQSVIARTRKENALGVGIYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKMADLLGSTHGYQWTDNAEEADVLLLNTCSIREKAQEKVFAMLGRWRLLKEKNPALIIGVG
GCVASQEGEHIRSRAPCVDVVFGPQTLHRLPEMLNHVQGTRSPIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TFCVVPYTRGEEVSRPSDDVLFEVAQLAAQGVREVNLLGQNVNAYRGATYDGGICSFAELLRLVAAIDGIDRIRYTTSHP
IEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRKARPDIQLSSDFIIGFPGETQADFEQT
MNLIAEINFDTSFSFIYSSRPGTPAADMVDDVSEDEKKQRLYILQDRINQQVLQFSRRMLGTVQRILVEGTSRKSVMELA
GRTACNRMVNFEGTPDMIGQFVDVEITEVLTNSLRGTVVRTEQQMDLRVHESPQSVIARTRKENALGVGIYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=36.5145228215768, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=36.5344467640919, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=28.6652078774617, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=84.3881856540084, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=428, Percent_Identity=28.9719626168224, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=33.0453563714903, Blast_Score=241, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=329, Percent_Identity=27.9635258358663, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=35.0840336134454, Blast_Score=274, Evalue=8e-74,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=29.3103448275862, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SERP5 (A8GB34)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477452.1
- ProteinModelPortal:   A8GB34
- SMR:   A8GB34
- STRING:   A8GB34
- GeneID:   5603498
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1220
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- BioCyc:   SPRO399741:SPRO_1220-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53280; Mature: 53148

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKMADLLGSTHGYQWTDNAEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
AMLGRWRLLKEKNPALIIGVGGCVASQEGEHIRSRAPCVDVVFGPQTLHRLPEMLNHVQG
HHHHHHHHHCCCCCEEEEECCCEECCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHCC
TRSPIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
VLFEVAQLAAQGVREVNLLGQNVNAYRGATYDGGICSFAELLRLVAAIDGIDRIRYTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PIEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QLSSDFIIGFPGETQADFEQTMNLIAEINFDTSFSFIYSSRPGTPAADMVDDVSEDEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCHHHH
RLYILQDRINQQVLQFSRRMLGTVQRILVEGTSRKSVMELAGRTACNRMVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
QFVDVEITEVLTNSLRGTVVRTEQQMDLRVHESPQSVIARTRKENALGVGIYQP
HHHCHHHHHHHHHHHCCCEEECCHHCCEEECCCHHHHHHHHHCCCCEEECCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKMADLLGSTHGYQWTDNAEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
AMLGRWRLLKEKNPALIIGVGGCVASQEGEHIRSRAPCVDVVFGPQTLHRLPEMLNHVQG
HHHHHHHHHCCCCCEEEEECCCEECCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHCC
TRSPIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
VLFEVAQLAAQGVREVNLLGQNVNAYRGATYDGGICSFAELLRLVAAIDGIDRIRYTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PIEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QLSSDFIIGFPGETQADFEQTMNLIAEINFDTSFSFIYSSRPGTPAADMVDDVSEDEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCHHHH
RLYILQDRINQQVLQFSRRMLGTVQRILVEGTSRKSVMELAGRTACNRMVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
QFVDVEITEVLTNSLRGTVVRTEQQMDLRVHESPQSVIARTRKENALGVGIYQP
HHHCHHHHHHHHHHHCCCEEECCHHCCEEECCCHHHHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA