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The map label for this gene is ushA [H]

Identifier: 157369390

GI number: 157369390

Start: 1264387

End: 1266042

Strand: Direct

Name: ushA [H]

Synonym: Spro_1147

Alternate gene names: 157369390

Gene position: 1264387-1266042 (Clockwise)

Preceding gene: 157369387

Following gene: 157369394

Centisome position: 23.2

GC content: 56.58

Gene sequence:

>1656_bases
ATGCGCTTTTCGTTAAAGACCACCGCATGCGCTTTGGCCGTGTCCCTGACTTTCCTGTCGGGAGCCGCCAGCGCCTGGGA
AAAGGATAAGACCTACGACATCACCATTTTGCATACCAACGATCACCACGGCCATTTCTGGCAGAACGACCACGGTGAAT
ATGGTCTGGGCGCACAAAAAACGCTGGTGGACGGCATTCGCCAGGAGGTCGCGGCCAAGGGCGGCAGCCTGTTGCTGCTG
TCGGGCGGTGATATTAATACCGGCGTACCGGAATCCGATCTGCAGGATGCCGAGCCGGACTTCCGCGGCATGAATCTGGT
GGGCTATGACGCGATGGCGATCGGCAACCATGAATTCGACAATCCGCTCAGCGTGTTGCGCCAGCAGGAGAAGTGGGCCA
CTTTCCCGCTGCTGTCGGCCAACATCTATCAGAAAAGCACCCAGCAGCGCCTGTTCAAACCCTATGCCTTGTTCGATAAG
CAGGGCATCAAGATCGCGGTGATCGGCCTGACTACCGATGACACCTCCAAGATTGGCAACCCGGAATATTTCACCGACAT
TGAGTTCCACGTACCGGCGCAGGAAGCCAAACAGGTGGTGGAGCAACTGCGCAAGGAAGAGAAACCGGACGTGATCATCG
CCGCGACCCACATGGGCCATTACGATGACGGCAACCACGGCTCCAATGCGCCCGGTGATGTAGAAATGGCGCGCAGCCTG
CCTGCCGGCTATCTGGATATGATCGTCGGTGGCCACTCGCAGGATCCGGTTTGTATGGCCGGTGAAAACCACAAGCAGGT
GGATTATGTGCCGGGCACCCCGTGCTCGCCGGATCGTCAGAACGGCACCTGGATCGTGCAGGCGCATGAGTGGGGCAAGT
ACGTGGGCCGTGCCGACTTCCAGTTCCGTAACGGTGAACTGAAGCTGGTGCATTATCAGCTGATCCCGGTCAATCTCAAG
AAGAAGGTAGAGAAAGCCGACGGTACCAGCGAGCGCGTCTATTACACCCAGCAGATCGCGGAAGATGCGTCGATGATGAA
ACTGCTGACGCCGTTCCAGGAAAAGGGCAAGGCGCAGCTGGACGTTAAAATTGGCAGCGTCAACGGCAAGCTGGAAGGCG
ATCGCAGTAAGGTGCGCTTTGTTCAGACCAACCTGTCACGCGTGCTGCTGGCAGCGCAGATGGAACGTGCCGAGGCCGAC
TTTGCCGTAATGAGCGGGGGCGGGGTACGTGATTCGATTGAAGCCGGTGACATTACCTATAAAAACGTGTTGAAGGTACA
GCCGTTTGGTAACACGTTGGTCTACGTCGAAATGAAAGGCAGCGAGGTGGAAAAATACCTGGCGGTGGTGGCTAATATGA
AAGTGGATTCCGGCGCCTACGCGCAGTTTGCCAACGTCAGCCTGACGGCGGACGGCAAGCAGGGCGTCAGCAATGTCAAA
ATCAAAGGCGAGCCGCTGCAGGCGGATAAAACTTACCGTATGGCCACGCTAAACTTCAATGCGCTGGGCGGTGACGGTTA
TCCGAAGCTGGATACTTTGCCAAGCTACGTCAACACCGGCTTTATCGATGCCGAAGTACTGAAACAATACATTGAGAAGC
ACTCACCGCTGGATGCGGCGGCCTATGAGCCGAAGGGCGAGATTGTTTATCAGTAA

Upstream 100 bases:

>100_bases
TTTGACGCACATCGCAAAAATTTGAAATTAGTTGCCTTTGTTTCGCGCTACAATTTCGGCCATCTCAAAAAAGCAGTTAA
ATATCATATGGAGTTCCGCC

Downstream 100 bases:

>100_bases
GTCGTCAGCGGGGGAGGCGTAAGTCTCCCCCTATCGATTCAGTCGCGCTTGGCGATATCGGCGAAGCTGCCGGCCAGCAG
CCGACAAAGATCCTCTGCCG

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MRFSLKTTACALAVSLTFLSGAASAWEKDKTYDITILHTNDHHGHFWQNDHGEYGLGAQKTLVDGIRQEVAAKGGSLLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLSVLRQQEKWATFPLLSANIYQKSTQQRLFKPYALFDK
QGIKIAVIGLTTDDTSKIGNPEYFTDIEFHVPAQEAKQVVEQLRKEEKPDVIIAATHMGHYDDGNHGSNAPGDVEMARSL
PAGYLDMIVGGHSQDPVCMAGENHKQVDYVPGTPCSPDRQNGTWIVQAHEWGKYVGRADFQFRNGELKLVHYQLIPVNLK
KKVEKADGTSERVYYTQQIAEDASMMKLLTPFQEKGKAQLDVKIGSVNGKLEGDRSKVRFVQTNLSRVLLAAQMERAEAD
FAVMSGGGVRDSIEAGDITYKNVLKVQPFGNTLVYVEMKGSEVEKYLAVVANMKVDSGAYAQFANVSLTADGKQGVSNVK
IKGEPLQADKTYRMATLNFNALGGDGYPKLDTLPSYVNTGFIDAEVLKQYIEKHSPLDAAAYEPKGEIVYQ

Sequences:

>Translated_551_residues
MRFSLKTTACALAVSLTFLSGAASAWEKDKTYDITILHTNDHHGHFWQNDHGEYGLGAQKTLVDGIRQEVAAKGGSLLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLSVLRQQEKWATFPLLSANIYQKSTQQRLFKPYALFDK
QGIKIAVIGLTTDDTSKIGNPEYFTDIEFHVPAQEAKQVVEQLRKEEKPDVIIAATHMGHYDDGNHGSNAPGDVEMARSL
PAGYLDMIVGGHSQDPVCMAGENHKQVDYVPGTPCSPDRQNGTWIVQAHEWGKYVGRADFQFRNGELKLVHYQLIPVNLK
KKVEKADGTSERVYYTQQIAEDASMMKLLTPFQEKGKAQLDVKIGSVNGKLEGDRSKVRFVQTNLSRVLLAAQMERAEAD
FAVMSGGGVRDSIEAGDITYKNVLKVQPFGNTLVYVEMKGSEVEKYLAVVANMKVDSGAYAQFANVSLTADGKQGVSNVK
IKGEPLQADKTYRMATLNFNALGGDGYPKLDTLPSYVNTGFIDAEVLKQYIEKHSPLDAAAYEPKGEIVYQ
>Mature_551_residues
MRFSLKTTACALAVSLTFLSGAASAWEKDKTYDITILHTNDHHGHFWQNDHGEYGLGAQKTLVDGIRQEVAAKGGSLLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLSVLRQQEKWATFPLLSANIYQKSTQQRLFKPYALFDK
QGIKIAVIGLTTDDTSKIGNPEYFTDIEFHVPAQEAKQVVEQLRKEEKPDVIIAATHMGHYDDGNHGSNAPGDVEMARSL
PAGYLDMIVGGHSQDPVCMAGENHKQVDYVPGTPCSPDRQNGTWIVQAHEWGKYVGRADFQFRNGELKLVHYQLIPVNLK
KKVEKADGTSERVYYTQQIAEDASMMKLLTPFQEKGKAQLDVKIGSVNGKLEGDRSKVRFVQTNLSRVLLAAQMERAEAD
FAVMSGGGVRDSIEAGDITYKNVLKVQPFGNTLVYVEMKGSEVEKYLAVVANMKVDSGAYAQFANVSLTADGKQGVSNVK
IKGEPLQADKTYRMATLNFNALGGDGYPKLDTLPSYVNTGFIDAEVLKQYIEKHSPLDAAAYEPKGEIVYQ

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=581, Percent_Identity=24.4406196213425, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1786687, Length=552, Percent_Identity=76.268115942029, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=575, Percent_Identity=22.2608695652174, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922446, Length=569, Percent_Identity=25.1318101933216, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24654424, Length=569, Percent_Identity=25.1318101933216, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28573524, Length=558, Percent_Identity=24.3727598566308, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24641187, Length=569, Percent_Identity=24.780316344464, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI221329836, Length=572, Percent_Identity=23.7762237762238, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922444, Length=567, Percent_Identity=23.1040564373898, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60641; Mature: 60641

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSLKTTACALAVSLTFLSGAASAWEKDKTYDITILHTNDHHGHFWQNDHGEYGLGAQK
CEEECHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHH
TLVDGIRQEVAAKGGSLLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLSVLRQQEKWATFPLLSANIYQKSTQQRLFKPYALFDKQGIKIAVIGLTTDDTSKIGN
CHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEECCCCCCCCC
PEYFTDIEFHVPAQEAKQVVEQLRKEEKPDVIIAATHMGHYDDGNHGSNAPGDVEMARSL
CCEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGYLDMIVGGHSQDPVCMAGENHKQVDYVPGTPCSPDRQNGTWIVQAHEWGKYVGRADF
CCCHHHHHCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
QFRNGELKLVHYQLIPVNLKKKVEKADGTSERVYYTQQIAEDASMMKLLTPFQEKGKAQL
EEECCCEEEEEEEEEECCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCHHHCCCEEE
DVKIGSVNGKLEGDRSKVRFVQTNLSRVLLAAQMERAEADFAVMSGGGVRDSIEAGDITY
EEEEECCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEE
KNVLKVQPFGNTLVYVEMKGSEVEKYLAVVANMKVDSGAYAQFANVSLTADGKQGVSNVK
HHEEEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCEEE
IKGEPLQADKTYRMATLNFNALGGDGYPKLDTLPSYVNTGFIDAEVLKQYIEKHSPLDAA
EECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCC
AYEPKGEIVYQ
CCCCCCCEEEC
>Mature Secondary Structure
MRFSLKTTACALAVSLTFLSGAASAWEKDKTYDITILHTNDHHGHFWQNDHGEYGLGAQK
CEEECHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHH
TLVDGIRQEVAAKGGSLLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLSVLRQQEKWATFPLLSANIYQKSTQQRLFKPYALFDKQGIKIAVIGLTTDDTSKIGN
CHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEECCCCCCCCC
PEYFTDIEFHVPAQEAKQVVEQLRKEEKPDVIIAATHMGHYDDGNHGSNAPGDVEMARSL
CCEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGYLDMIVGGHSQDPVCMAGENHKQVDYVPGTPCSPDRQNGTWIVQAHEWGKYVGRADF
CCCHHHHHCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
QFRNGELKLVHYQLIPVNLKKKVEKADGTSERVYYTQQIAEDASMMKLLTPFQEKGKAQL
EEECCCEEEEEEEEEECCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCHHHCCCEEE
DVKIGSVNGKLEGDRSKVRFVQTNLSRVLLAAQMERAEADFAVMSGGGVRDSIEAGDITY
EEEEECCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEE
KNVLKVQPFGNTLVYVEMKGSEVEKYLAVVANMKVDSGAYAQFANVSLTADGKQGVSNVK
HHEEEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCEEE
IKGEPLQADKTYRMATLNFNALGGDGYPKLDTLPSYVNTGFIDAEVLKQYIEKHSPLDAA
EECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCC
AYEPKGEIVYQ
CCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA