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The map label for this gene is cof

Identifier: 157369347

GI number: 157369347

Start: 1215520

End: 1216344

Strand: Direct

Name: cof

Synonym: Spro_1104

Alternate gene names: 157369347

Gene position: 1215520-1216344 (Clockwise)

Preceding gene: 157369344

Following gene: 157369349

Centisome position: 22.31

GC content: 58.42

Gene sequence:

>825_bases
ATGTATCGCCTGGCGGCTTTTGATATGGATGGCACTTTGTTGACACCGGATCACCGGGTCGGGCCGGAGACTCTGGCGGT
GCTAAAGCAACTTGTTGAACGGGAGATGGTGGTGACTTTCGCCACCGGGCGCCACTACCTTGATGCACAGCCGATCATGG
CGCAGCTGGGCCTGCAGGGTTATCTGATTACCGGCAACGGTACGCGGGTCTATGACGACCGGGGGCAGCAACTGCAGGCT
ACCGATTTGCCGGCCGATATTGCCGAAGAGGTGCTGCATACTCACTGGCATACCGACGCCAGCATGCACGTCTTCCGTGA
TGAAGGCTGGATGACCGAGTTTGCGGTGCCGGAGGAAATGTTGCGGGCCCACCACCTGAGCGGCTTCCATTATCAGCTTA
CCGAACTGCGGCGTTTACCGGCGTTCGGCAACAGCAAGGTTTGTTTTGCCGGGCCGCATGAGGAACTGCTGAAACTTCAG
GTGCAGCTGCGCCGGCACTTTGCTGCGCGGGTTGATCTCTGTTTCTCGGCCTATGAATGCCTGGAAGTCTTGCCGCTGGG
CTGCAACAAGGGCTCGGCGCTGGATATGCTGAGCCGCCATCTGGGGCTGAAGATGGCGGAGTGTATGGCGTTTGGTGATG
CGATGAACGACAAGGAAATGCTGGCGACGGTAGGGCACGGGGTGGTAATGGGCAACGCCCTGCCACAGCTCAAGTCGCTA
TTGCCACAGCTACAGGTTATCGGCCACTGCGAACAGCAGGCGGTGGCCCACTATTTGCAACATTGGCTGCGTTCACCTTA
CCTCACCTATTCCCCCGAATTATGA

Upstream 100 bases:

>100_bases
GGAAATCCGGAACAGTTTTCCAGAATTGTTCACTATTACTTCCGTAAATACCATCCCATACTGCACGGACTGTAATCGCG
TTCACATTCACGAGGTTTTT

Downstream 100 bases:

>100_bases
GGCTTTATTGGCAGCCAGCCGGGTATGACTTACCCGGTTTTTTTTCGTCTGAACCGGCTGATTACAAATCTGCTAACTGC
GCCAGATAAGGCGTCAGATC

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MYRLAAFDMDGTLLTPDHRVGPETLAVLKQLVEREMVVTFATGRHYLDAQPIMAQLGLQGYLITGNGTRVYDDRGQQLQA
TDLPADIAEEVLHTHWHTDASMHVFRDEGWMTEFAVPEEMLRAHHLSGFHYQLTELRRLPAFGNSKVCFAGPHEELLKLQ
VQLRRHFAARVDLCFSAYECLEVLPLGCNKGSALDMLSRHLGLKMAECMAFGDAMNDKEMLATVGHGVVMGNALPQLKSL
LPQLQVIGHCEQQAVAHYLQHWLRSPYLTYSPEL

Sequences:

>Translated_274_residues
MYRLAAFDMDGTLLTPDHRVGPETLAVLKQLVEREMVVTFATGRHYLDAQPIMAQLGLQGYLITGNGTRVYDDRGQQLQA
TDLPADIAEEVLHTHWHTDASMHVFRDEGWMTEFAVPEEMLRAHHLSGFHYQLTELRRLPAFGNSKVCFAGPHEELLKLQ
VQLRRHFAARVDLCFSAYECLEVLPLGCNKGSALDMLSRHLGLKMAECMAFGDAMNDKEMLATVGHGVVMGNALPQLKSL
LPQLQVIGHCEQQAVAHYLQHWLRSPYLTYSPEL
>Mature_274_residues
MYRLAAFDMDGTLLTPDHRVGPETLAVLKQLVEREMVVTFATGRHYLDAQPIMAQLGLQGYLITGNGTRVYDDRGQQLQA
TDLPADIAEEVLHTHWHTDASMHVFRDEGWMTEFAVPEEMLRAHHLSGFHYQLTELRRLPAFGNSKVCFAGPHEELLKLQ
VQLRRHFAARVDLCFSAYECLEVLPLGCNKGSALDMLSRHLGLKMAECMAFGDAMNDKEMLATVGHGVVMGNALPQLKSL
LPQLQVIGHCEQQAVAHYLQHWLRSPYLTYSPEL

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P)

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family

Homologues:

Organism=Escherichia coli, GI87081741, Length=273, Percent_Identity=57.1428571428571, Blast_Score=330, Evalue=4e-92,
Organism=Escherichia coli, GI48994981, Length=262, Percent_Identity=41.6030534351145, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786982, Length=279, Percent_Identity=28.673835125448, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COF_SERP5 (A8GAR8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477336.1
- ProteinModelPortal:   A8GAR8
- SMR:   A8GAR8
- STRING:   A8GAR8
- GeneID:   5605474
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1104
- eggNOG:   COG0561
- HOGENOM:   HBG728500
- OMA:   NECIPEV
- ProtClustDB:   PRK15126
- BioCyc:   SPRO399741:SPRO_1104-MONOMER
- HAMAP:   MF_01847
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR00099
- TIGRFAMs:   TIGR01484

Pfam domain/function: PF08282 Hydrolase_3; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 30878; Mature: 30878

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01228 COF_1; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLAAFDMDGTLLTPDHRVGPETLAVLKQLVEREMVVTFATGRHYLDAQPIMAQLGLQG
CCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCEECCCCHHHHHCCCCE
YLITGNGTRVYDDRGQQLQATDLPADIAEEVLHTHWHTDASMHVFRDEGWMTEFAVPEEM
EEEECCCCEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEHHCCHHHH
LRAHHLSGFHYQLTELRRLPAFGNSKVCFAGPHEELLKLQVQLRRHFAARVDLCFSAYEC
HHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEVLPLGCNKGSALDMLSRHLGLKMAECMAFGDAMNDKEMLATVGHGVVMGNALPQLKSL
HHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCCEECCHHHHHHHH
LPQLQVIGHCEQQAVAHYLQHWLRSPYLTYSPEL
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MYRLAAFDMDGTLLTPDHRVGPETLAVLKQLVEREMVVTFATGRHYLDAQPIMAQLGLQG
CCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCEECCCCHHHHHCCCCE
YLITGNGTRVYDDRGQQLQATDLPADIAEEVLHTHWHTDASMHVFRDEGWMTEFAVPEEM
EEEECCCCEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEHHCCHHHH
LRAHHLSGFHYQLTELRRLPAFGNSKVCFAGPHEELLKLQVQLRRHFAARVDLCFSAYEC
HHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEVLPLGCNKGSALDMLSRHLGLKMAECMAFGDAMNDKEMLATVGHGVVMGNALPQLKSL
HHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCCEECCHHHHHHHH
LPQLQVIGHCEQQAVAHYLQHWLRSPYLTYSPEL
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA