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The map label for this gene is rbsB [H]

Identifier: 157369274

GI number: 157369274

Start: 1133759

End: 1134703

Strand: Direct

Name: rbsB [H]

Synonym: Spro_1031

Alternate gene names: 157369274

Gene position: 1133759-1134703 (Clockwise)

Preceding gene: 157369273

Following gene: 157369275

Centisome position: 20.81

GC content: 58.1

Gene sequence:

>945_bases
ATGCGTTTCAAGCCATTGATTACCGCTTTGCTGGTTACTGCCGCGCTCGGCAGTGCCTCGTTGGTGCAGGCAAAAGAGTT
GAAATCGATTGGGGTGACGGTGGGCGATTTGGCCAACCCGTTCTTCGTACAGATCACCAAGGGTGCAGAAATGAAGGCGC
GCGAGTTGGCCGGCGATAAGGTCAACGTGACGCTGGTGTCGAGCGGTTACGATCTCGGCCAGCAGGTGGCGCAGATCGAT
AACTTTATCGCTGCCAAGGTTGACATGATCATCCTGAACGCCGCGGATTCCAAGGGTATCGGCCCGGCGGTAAAACGCGC
CAAGGATGCCGGTATCGTGGTAGTGGCGGTTGACGTGGCGGCCGAAGGTGCCGATGCCACCATCACCTCAGATAACACCC
AGGCCGGCGCGATGGCCTGTAAATACATCAGCGACCGCCTGAAAGAGAAAGGCAACGTGGTGATCATCAACGGGCCACCG
GTGTCCGCGGTACAAAACCGGGTCGAAGGCTGCATGACCGAGTTGAAGAAACACCCGGAGATCAAACTGCTGTCTTATAA
CCAGAATGCCAAGGGCAGCCGTGAAGGTGGGCTGGAGATCATGACCGCGCTGTTGGCGGCCAATCCGAAGATCGACGGCG
TGTTTGCGATTAACGATCCTACGGCGATCGGCGCCGATCTGGCTGCCAAACAGGCGCAGCGCAGCGAGTTCTTTATCGTT
GGCGTGGATGGATCGCCGGACGGGGAAGAGGCGCTCAAGCGTGGCGGGAAATCGCTGTTTGTCGCGACGCCGGCGCAGGA
TCCTCAGGTGATGGCGGCCAAGGCGGTCGAGATCGGTTACGACATTCTGCAGGGTAAACCTGCGCCAACCGCGCCGGTGC
TGATCCCGGTAACCATGATCGATAAAAACAATATCGGCAGCTACAAAGGCTGGACGGTGAAATAA

Upstream 100 bases:

>100_bases
ACTTACCCGGTGTCTTTCAAGCTGCGGGGTGAGTGATAAAGAAAGGTCGGCGCCCACCGGGGCAGGTTTTCGTAGATCGG
AAAATAATGAGGAAAACACT

Downstream 100 bases:

>100_bases
CCTGTTATCGGCGGGGCCCGGCCAGACTGGGCCCCTCATTCCCCTACCGCTTGCGGAGGTACCATGAACCGTTCCGCCGT
GAATCAAATTCTGCAGCAAA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRFKPLITALLVTAALGSASLVQAKELKSIGVTVGDLANPFFVQITKGAEMKARELAGDKVNVTLVSSGYDLGQQVAQID
NFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPP
VSAVQNRVEGCMTELKKHPEIKLLSYNQNAKGSREGGLEIMTALLAANPKIDGVFAINDPTAIGADLAAKQAQRSEFFIV
GVDGSPDGEEALKRGGKSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPTAPVLIPVTMIDKNNIGSYKGWTVK

Sequences:

>Translated_314_residues
MRFKPLITALLVTAALGSASLVQAKELKSIGVTVGDLANPFFVQITKGAEMKARELAGDKVNVTLVSSGYDLGQQVAQID
NFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPP
VSAVQNRVEGCMTELKKHPEIKLLSYNQNAKGSREGGLEIMTALLAANPKIDGVFAINDPTAIGADLAAKQAQRSEFFIV
GVDGSPDGEEALKRGGKSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPTAPVLIPVTMIDKNNIGSYKGWTVK
>Mature_314_residues
MRFKPLITALLVTAALGSASLVQAKELKSIGVTVGDLANPFFVQITKGAEMKARELAGDKVNVTLVSSGYDLGQQVAQID
NFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPP
VSAVQNRVEGCMTELKKHPEIKLLSYNQNAKGSREGGLEIMTALLAANPKIDGVFAINDPTAIGADLAAKQAQRSEFFIV
GVDGSPDGEEALKRGGKSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPTAPVLIPVTMIDKNNIGSYKGWTVK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=289, Percent_Identity=33.5640138408304, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790526, Length=288, Percent_Identity=28.4722222222222, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790715, Length=276, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789990, Length=304, Percent_Identity=30.2631578947368, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32784; Mature: 32784

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKPLITALLVTAALGSASLVQAKELKSIGVTVGDLANPFFVQITKGAEMKARELAGDK
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCHHHHHHCCCE
VNVTLVSSGYDLGQQVAQIDNFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVA
EEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEEE
AEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPPVSAVQNRVEGCMTELKKHPE
CCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
IKLLSYNQNAKGSREGGLEIMTALLAANPKIDGVFAINDPTAIGADLAAKQAQRSEFFIV
EEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
GVDGSPDGEEALKRGGKSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPTAPVLIPVTMI
EECCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
DKNNIGSYKGWTVK
ECCCCCCCCCCEEC
>Mature Secondary Structure
MRFKPLITALLVTAALGSASLVQAKELKSIGVTVGDLANPFFVQITKGAEMKARELAGDK
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCHHHHHHCCCE
VNVTLVSSGYDLGQQVAQIDNFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVA
EEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEEE
AEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPPVSAVQNRVEGCMTELKKHPE
CCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
IKLLSYNQNAKGSREGGLEIMTALLAANPKIDGVFAINDPTAIGADLAAKQAQRSEFFIV
EEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
GVDGSPDGEEALKRGGKSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPTAPVLIPVTMI
EECCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
DKNNIGSYKGWTVK
ECCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]