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The map label for this gene is btuF [H]

Identifier: 157369031

GI number: 157369031

Start: 874856

End: 875662

Strand: Reverse

Name: btuF [H]

Synonym: Spro_0786

Alternate gene names: 157369031

Gene position: 875662-874856 (Counterclockwise)

Preceding gene: 157369032

Following gene: 157369030

Centisome position: 16.07

GC content: 62.33

Gene sequence:

>807_bases
GTGACGCGACGCTTTTCAGCCCTGCTGTGGCTGCTGGCATTATGGATGGCGCTGCCCGTCGGCGCCGCCCAACGGGTGAT
CAGCCTGGCACCCAACACCACCGAGCTTGCCTATGCTGCCGGCATGGGCGATACCCTGCTGGCAGTAAGCGCTTATTCCG
ACTATCCCCCGGCGGCCAGCAAGCTGGAGCAAGTCGCTTCCTGGCAAGGCATCAACCTGGAGCGGGTGCTGGCGCTAAAG
CCGGATCTGATCCTGGCCTGGCGCGGCGGCAATCCACAACGGGTGCTGGATCAGATCGCCTCTTTTGGCATCCCGATCTT
TTATGCCGATGCCACCACCATCGATGACATCGCGCAGCGGCTGGATGACCTGGCCCAATACAGCCCACACCCAGAACAAG
CGCATCAGGCCGCGAACGCGATCCGTCAGCAGATTGCCGCGCTGAAGGCAAAATATCCGCACAGTCAGCCGCGGCGGGTT
CTGCTGCAATTCGGCACCCAGCCGCTGTTCACCAGCTCTGGCGCGACGTTGCAAAGTCAGGTGTTGTCGTTGTGCGGCGC
AGAGAACATCTTTGCCGACAGCCGCATGCCGTGGCCGCAAATCAGCCGCGAGCAGGTACTGGCCCGCCAACCGCAGGCCA
TCGTCATTACCGGCGACCAAAAAGAAATCGCCAGCGTGAAAGCCTTCTGGGCGCCGCAACTGCAGGTACCGGTGCTTGCG
GTGAATGGCGACTGGTTCAACCGCGCCGGCCCACGCATCATGCTGGCGGCCCAGCAACTGTGCAGCCAACTGGCGGAGAT
TCATTAA

Upstream 100 bases:

>100_bases
GAATCGCACATGAGTTTTGAGGAGTTCCTTGTCGTGGCGGCCAAGCAATCTACGCTGATGGTCAACGCCATGCTGCAGAC
TCTGGCTAAGCGCGGTTGAT

Downstream 100 bases:

>100_bases
TGCTGGTGTTCTGGCTGGATATTCTCGGCACTGCGGTTTTCGCCATCTCCGGCGTGTTGTTGGCAGGCAAGTTGCGGATG
GATCCGTTCGGCGTACTGGT

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTRRFSALLWLLALWMALPVGAAQRVISLAPNTTELAYAAGMGDTLLAVSAYSDYPPAASKLEQVASWQGINLERVLALK
PDLILAWRGGNPQRVLDQIASFGIPIFYADATTIDDIAQRLDDLAQYSPHPEQAHQAANAIRQQIAALKAKYPHSQPRRV
LLQFGTQPLFTSSGATLQSQVLSLCGAENIFADSRMPWPQISREQVLARQPQAIVITGDQKEIASVKAFWAPQLQVPVLA
VNGDWFNRAGPRIMLAAQQLCSQLAEIH

Sequences:

>Translated_268_residues
MTRRFSALLWLLALWMALPVGAAQRVISLAPNTTELAYAAGMGDTLLAVSAYSDYPPAASKLEQVASWQGINLERVLALK
PDLILAWRGGNPQRVLDQIASFGIPIFYADATTIDDIAQRLDDLAQYSPHPEQAHQAANAIRQQIAALKAKYPHSQPRRV
LLQFGTQPLFTSSGATLQSQVLSLCGAENIFADSRMPWPQISREQVLARQPQAIVITGDQKEIASVKAFWAPQLQVPVLA
VNGDWFNRAGPRIMLAAQQLCSQLAEIH
>Mature_267_residues
TRRFSALLWLLALWMALPVGAAQRVISLAPNTTELAYAAGMGDTLLAVSAYSDYPPAASKLEQVASWQGINLERVLALKP
DLILAWRGGNPQRVLDQIASFGIPIFYADATTIDDIAQRLDDLAQYSPHPEQAHQAANAIRQQIAALKAKYPHSQPRRVL
LQFGTQPLFTSSGATLQSQVLSLCGAENIFADSRMPWPQISREQVLARQPQAIVITGDQKEIASVKAFWAPQLQVPVLAV
NGDWFNRAGPRIMLAAQQLCSQLAEIH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=268, Percent_Identity=55.2238805970149, Blast_Score=304, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29373; Mature: 29242

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRFSALLWLLALWMALPVGAAQRVISLAPNTTELAYAAGMGDTLLAVSAYSDYPPAAS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHH
KLEQVASWQGINLERVLALKPDLILAWRGGNPQRVLDQIASFGIPIFYADATTIDDIAQR
HHHHHHHCCCCCHHHHEEECCCEEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LDDLAQYSPHPEQAHQAANAIRQQIAALKAKYPHSQPRRVLLQFGTQPLFTSSGATLQSQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
VLSLCGAENIFADSRMPWPQISREQVLARQPQAIVITGDQKEIASVKAFWAPQLQVPVLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEE
VNGDWFNRAGPRIMLAAQQLCSQLAEIH
ECCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRRFSALLWLLALWMALPVGAAQRVISLAPNTTELAYAAGMGDTLLAVSAYSDYPPAAS
CHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHH
KLEQVASWQGINLERVLALKPDLILAWRGGNPQRVLDQIASFGIPIFYADATTIDDIAQR
HHHHHHHCCCCCHHHHEEECCCEEEEECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LDDLAQYSPHPEQAHQAANAIRQQIAALKAKYPHSQPRRVLLQFGTQPLFTSSGATLQSQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
VLSLCGAENIFADSRMPWPQISREQVLARQPQAIVITGDQKEIASVKAFWAPQLQVPVLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEE
VNGDWFNRAGPRIMLAAQQLCSQLAEIH
ECCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA