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The map label for this gene is leuD

Identifier: 157368987

GI number: 157368987

Start: 825749

End: 826351

Strand: Reverse

Name: leuD

Synonym: Spro_0742

Alternate gene names: 157368987

Gene position: 826351-825749 (Counterclockwise)

Preceding gene: 157368988

Following gene: 157368983

Centisome position: 15.17

GC content: 53.9

Gene sequence:

>603_bases
GTGGCTAAATTTACTCAACATACCGGCTTAGTGGTGCCTTTGGATGCGGCGAACGTCGATACCGATGCCATTATTCCAAA
ACAGTTTTTGCAGAAGGTGACGCGCACCGGTTTTGGTAAGCACCTGTTTAACGACTGGCGTTTTCTGGACGATGCCGGCC
AGCAGCCAAACCCGGAGTTTGTCCTGAATAAACCGCGCTACAAAGGTGCCAGCATTTTGCTGGCCCGGGAAAACTTCGGC
TGCGGCTCATCACGCGAACACGCGCCCTGGGCGCTGACCGACTATGGTTTTAGAGTGGTGATTGCCCCCAGCTTTGCCGA
TATCTTCTACGGCAACTCGCTCAATAACCAGCTGCTGCCGGTTAAGCTGAGCGAACAGGACGTGGAAACGCTGTTCCAGC
TGGTGGCTGCCAACGAAGGCATTGAATTTGAAGTTGATTTGGAAAACCAGACGGTCAAAGCCGGTGGCAAAAGCTATCCG
TTCGATATTGATAGCTTCCGCCGTCACTGCATGATTAACGGGCTGGACAGTATCGGCCTGACGCTGCAGCACGAAGCGGA
TATTTCCCGCTACGAGGCGCAGCAACCCGCCTTCCTGAACTGA

Upstream 100 bases:

>100_bases
GCGGTGGGCGTACTCACCTGGTCAGCCCGGCAATGGCCGCTGCGGCCGCTATTGCCGGCCATTTCGCCGATATCCGTGAT
ATTCACTAAGGAAGGCTACC

Downstream 100 bases:

>100_bases
TATTAATCACCCGCCACCTGGCGGGTGTTTTTTTATTATTAATAACCGCTTACCAGTAGCCCAGCCACTTCCACCACGCT
CCACCAATCAATATCCAAAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MAKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGKHLFNDWRFLDDAGQQPNPEFVLNKPRYKGASILLARENFG
CGSSREHAPWALTDYGFRVVIAPSFADIFYGNSLNNQLLPVKLSEQDVETLFQLVAANEGIEFEVDLENQTVKAGGKSYP
FDIDSFRRHCMINGLDSIGLTLQHEADISRYEAQQPAFLN

Sequences:

>Translated_200_residues
MAKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGKHLFNDWRFLDDAGQQPNPEFVLNKPRYKGASILLARENFG
CGSSREHAPWALTDYGFRVVIAPSFADIFYGNSLNNQLLPVKLSEQDVETLFQLVAANEGIEFEVDLENQTVKAGGKSYP
FDIDSFRRHCMINGLDSIGLTLQHEADISRYEAQQPAFLN
>Mature_199_residues
AKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGKHLFNDWRFLDDAGQQPNPEFVLNKPRYKGASILLARENFGC
GSSREHAPWALTDYGFRVVIAPSFADIFYGNSLNNQLLPVKLSEQDVETLFQLVAANEGIEFEVDLENQTVKAGGKSYPF
DIDSFRRHCMINGLDSIGLTLQHEADISRYEAQQPAFLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=81.8181818181818, Blast_Score=343, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=51.7587939698493, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SERP5 (A8G9Q8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476976.1
- STRING:   A8G9Q8
- GeneID:   5604903
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0742
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   SPRO399741:SPRO_0742-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22409; Mature: 22278

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGKHLFNDWRFLDDAGQQPNPEF
CCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VLNKPRYKGASILLARENFGCGSSREHAPWALTDYGFRVVIAPSFADIFYGNSLNNQLLP
EEECCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCEEE
VKLSEQDVETLFQLVAANEGIEFEVDLENQTVKAGGKSYPFDIDSFRRHCMINGLDSIGL
EEECHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCE
TLQHEADISRYEAQQPAFLN
EEECCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
AKFTQHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGKHLFNDWRFLDDAGQQPNPEF
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VLNKPRYKGASILLARENFGCGSSREHAPWALTDYGFRVVIAPSFADIFYGNSLNNQLLP
EEECCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCEEE
VKLSEQDVETLFQLVAANEGIEFEVDLENQTVKAGGKSYPFDIDSFRRHCMINGLDSIGL
EEECHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCE
TLQHEADISRYEAQQPAFLN
EEECCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA