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The map label for this gene is tbpA [H]

Identifier: 157368982

GI number: 157368982

Start: 819616

End: 820602

Strand: Reverse

Name: tbpA [H]

Synonym: Spro_0737

Alternate gene names: 157368982

Gene position: 820602-819616 (Counterclockwise)

Preceding gene: 157368983

Following gene: 157368981

Centisome position: 15.06

GC content: 56.53

Gene sequence:

>987_bases
GTGGTCAAAAAAAGCCTGCCCTTGTTACTGTTGTTGTGCGCCGCACCGGTGTTCGCCAAACCGGTACTGACGGTCTACAC
CTATGATTCTTTCGCTGCCGACTGGGGCCCTGGCCCGGCGGTCAAAAAAGCCTTTGAAGCCCAGTGCGACTGTGAGCTGA
AATTTGTCGCGCTGGAAGACGGCGTTTCGCTGCTTAACCGCCTGCGCATGGAAGGAAAAAACAGCGCCGCCGACGTGGTG
TTGGGGCTGGACAACAATTTGCTGCAGGCCGCGCAACAAACCGGTTTATTTGCGCCGAGCAACGTCGACAGCACCAAACT
GAGCGTTCCCGGCGGCTGGCAGGATAAAACCTTCGTGCCTTATGACTACGGCTACTTTGCCTTCGTTTACAACAAAGAAA
AACTGAAGAATCCGCCCAAAAGCCTGCAAGAGTTGGTCGACAGTGACCAGAACTGGAAGGTGATTTACGAAGATCCGCGT
ACCAGCACGCCGGGCCTCGGCCTGCTGTTGTGGATGCAGAAAGTCTATGGCGACAAGGCCCCCGAAGCCTGGCAAAAGCT
GGCAAAGAAAACCGTCACCGTGACCAAGGGCTGGAGTGAGGCTTACGGCCTGTTCCTGAAAGGCGAAGGCGACCTGGTAC
TCAGCTACACCACCTCTCCGGCCTACCATCTGATTGAAGAAAAGAAAGACAGCTACGCCGCCGCTAACTTCAGCGAAGGC
CACTACCTGCAGGTCGAGGTGGCAGGCAAACTCAAGGCCGGCAAACAGCCGGAGTTGGCAGAGCGCTTTATGCAATTTAT
GGTGACCCCGGCGTTCCAGAACAGCATTCCCACCGGCAACTGGATGTATCCGGTGATCAAAACCCAGCTGCCGGCCGGGT
TTGAGCAAATGACCGTACCGCAGACCGCGTTGCAATACAGCGCTGAAGAAGTGGCCAAACAACGCGGCAACTGGATCCGG
GCATGGCAAACCGCCGTCAGCCACTGA

Upstream 100 bases:

>100_bases
ATCCGGTCAATACCGGCGGAGGGATTTGAGCCCAGCGAACCGGCAGCCGCCGCTCGCCCCCTCAGATACCCTTGCCCCCC
TAAATTTTCAGGAGTGCAAA

Downstream 100 bases:

>100_bases
TCCCACGCTGGCTATGGCCGGGGCTGCTGGCTTCCGGTCTGATCCTGACCATCGCCGTACTGGCGATGGGTTCGCTGTGG
CGACACGCGCCCGAAAGCGA

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MVKKSLPLLLLLCAAPVFAKPVLTVYTYDSFAADWGPGPAVKKAFEAQCDCELKFVALEDGVSLLNRLRMEGKNSAADVV
LGLDNNLLQAAQQTGLFAPSNVDSTKLSVPGGWQDKTFVPYDYGYFAFVYNKEKLKNPPKSLQELVDSDQNWKVIYEDPR
TSTPGLGLLLWMQKVYGDKAPEAWQKLAKKTVTVTKGWSEAYGLFLKGEGDLVLSYTTSPAYHLIEEKKDSYAAANFSEG
HYLQVEVAGKLKAGKQPELAERFMQFMVTPAFQNSIPTGNWMYPVIKTQLPAGFEQMTVPQTALQYSAEEVAKQRGNWIR
AWQTAVSH

Sequences:

>Translated_328_residues
MVKKSLPLLLLLCAAPVFAKPVLTVYTYDSFAADWGPGPAVKKAFEAQCDCELKFVALEDGVSLLNRLRMEGKNSAADVV
LGLDNNLLQAAQQTGLFAPSNVDSTKLSVPGGWQDKTFVPYDYGYFAFVYNKEKLKNPPKSLQELVDSDQNWKVIYEDPR
TSTPGLGLLLWMQKVYGDKAPEAWQKLAKKTVTVTKGWSEAYGLFLKGEGDLVLSYTTSPAYHLIEEKKDSYAAANFSEG
HYLQVEVAGKLKAGKQPELAERFMQFMVTPAFQNSIPTGNWMYPVIKTQLPAGFEQMTVPQTALQYSAEEVAKQRGNWIR
AWQTAVSH
>Mature_328_residues
MVKKSLPLLLLLCAAPVFAKPVLTVYTYDSFAADWGPGPAVKKAFEAQCDCELKFVALEDGVSLLNRLRMEGKNSAADVV
LGLDNNLLQAAQQTGLFAPSNVDSTKLSVPGGWQDKTFVPYDYGYFAFVYNKEKLKNPPKSLQELVDSDQNWKVIYEDPR
TSTPGLGLLLWMQKVYGDKAPEAWQKLAKKTVTVTKGWSEAYGLFLKGEGDLVLSYTTSPAYHLIEEKKDSYAAANFSEG
HYLQVEVAGKLKAGKQPELAERFMQFMVTPAFQNSIPTGNWMYPVIKTQLPAGFEQMTVPQTALQYSAEEVAKQRGNWIR
AWQTAVSH

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=314, Percent_Identity=79.6178343949045, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36498; Mature: 36498

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKSLPLLLLLCAAPVFAKPVLTVYTYDSFAADWGPGPAVKKAFEAQCDCELKFVALED
CCCCHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEEHH
GVSLLNRLRMEGKNSAADVVLGLDNNLLQAAQQTGLFAPSNVDSTKLSVPGGWQDKTFVP
HHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEECE
YDYGYFAFVYNKEKLKNPPKSLQELVDSDQNWKVIYEDPRTSTPGLGLLLWMQKVYGDKA
ECCCEEEEEEEHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCC
PEAWQKLAKKTVTVTKGWSEAYGLFLKGEGDLVLSYTTSPAYHLIEEKKDSYAAANFSEG
HHHHHHHHHHEEEEECCCHHHCEEEEECCCCEEEEEECCCHHHHHHHHCCCCEECCCCCC
HYLQVEVAGKLKAGKQPELAERFMQFMVTPAFQNSIPTGNWMYPVIKTQLPAGFEQMTVP
CEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCEECHHHHCCCCCCHHHHCCC
QTALQYSAEEVAKQRGNWIRAWQTAVSH
HHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MVKKSLPLLLLLCAAPVFAKPVLTVYTYDSFAADWGPGPAVKKAFEAQCDCELKFVALED
CCCCHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEEHH
GVSLLNRLRMEGKNSAADVVLGLDNNLLQAAQQTGLFAPSNVDSTKLSVPGGWQDKTFVP
HHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEECE
YDYGYFAFVYNKEKLKNPPKSLQELVDSDQNWKVIYEDPRTSTPGLGLLLWMQKVYGDKA
ECCCEEEEEEEHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCC
PEAWQKLAKKTVTVTKGWSEAYGLFLKGEGDLVLSYTTSPAYHLIEEKKDSYAAANFSEG
HHHHHHHHHHEEEEECCCHHHCEEEEECCCCEEEEEECCCHHHHHHHHCCCCEECCCCCC
HYLQVEVAGKLKAGKQPELAERFMQFMVTPAFQNSIPTGNWMYPVIKTQLPAGFEQMTVP
CEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCEECHHHHCCCCCCHHHHCCC
QTALQYSAEEVAKQRGNWIRAWQTAVSH
HHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]