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The map label for this gene is thiP [H]

Identifier: 157368981

GI number: 157368981

Start: 818033

End: 819640

Strand: Reverse

Name: thiP [H]

Synonym: Spro_0736

Alternate gene names: 157368981

Gene position: 819640-818033 (Counterclockwise)

Preceding gene: 157368982

Following gene: 157368980

Centisome position: 15.04

GC content: 61.57

Gene sequence:

>1608_bases
ATGGCAAACCGCCGTCAGCCACTGATCCCACGCTGGCTATGGCCGGGGCTGCTGGCTTCCGGTCTGATCCTGACCATCGC
CGTACTGGCGATGGGTTCGCTGTGGCGACACGCGCCCGAAAGCGACTGGCAGGGTCTGTGGCAGGACAGCTACCTGTGGC
ACGTGGTGCGTTTCACCTTCTGGCAGGCGTTGCTGTCGGCACTGATCTCGGTGCTGCCCGCTATCTTGCTGGCGCGAGCG
CTGTTCCGTCGCCGTTTCCCTGGCCGCCAATTGCTGCTACGGCTGTGCGCCATGACGCTGGTGCTGCCGGTACTGGTGGC
GGTGTTCGGTCTGCTCAGTGTTTATGGCCGACAAGGCTGGCTGGCCGCAATATGCGGTTGGCTCGGCATTAACTACAGTT
TCTCCCCCTATGGCCTGCAGGGCATTCTGTTGGCCCATATGTTCTTCAACCTGCCGCTGGCGACCCGCCTGCTGTTACAG
GCGCTGGAAAACATTCCGGTCGAACAGCGTCAACTGGCTGCGCAGCTTGGCATGAGCGGGCGGCAGCAGTTCCGCTTTGT
CGAGTGGCCGGCACTACGCCGCCAGATCCTGCCGAGCGGTGCGCTGATCTTTATGCTGTGCTTTGCCAGTTTCGCCACCG
TATTGTCACTGGGCGGCGGGCCGCAGGCCACCACCATCGAACTGGCGATCTACCAGGCACTCAGCTACGACTACGATCTC
AGCCGCGCCGCGCTATTGGCGCTGATCCAACTGGCCTGCTGCCTGGGGCTGGTGGTGCTCAGCCAGCGGTTAAGCCAGGC
ACTCCCCGTTGGTAACACGCACGCCCACATCTGGCGTAATCCGGAAGACAGCCTGTGGCGCCGCGTTAGCGATTTTCTGC
TGATCGCTGCCGCCCTGCTACTGCTGTTACCCCCACTGCTGGCGGTGATTGCCGACGGTGCCAACCAAAGCCTGGTCAGC
GTGCTGCAACAGCCTGTGCTGTGGCAGGCGTTATTCACCTCATTACGCATTGCTCTCGGTGCCGGCGTACTGTGCGTGCT
ACTGACCATGATGCTGCTGTGGAGCAGTCGTGAGCTGAAGCTGCAACAGCGGCTGCGCTGGGGCCAACGACTTGAACTAA
GTGGCATGGTGATCCTGGCAATGCCAGGCATCGTGCTGGCGACCGGTTTCTTCCTGCTGTTGAGTGACACCGTCGGGCTG
CCGCAGTCGCCGTATGCGCTGGTGATCCTTACCAATGCACTGATGGCGGTGCCCTACGCGCTGAAAGTGCTGGAAAACCC
GATGCGCGACCTGGCCGAACGCTACAACCCACTGTGCCTGTCGCTGGATATCCACGGCTGGCAGCGACTGCGGCTTATCG
AACTGAAAGCATTGCGCCGCCCACTGGCCCAGGCGCTGGCCTTCGCCTGCGTACTGTCGATTGGCGATTTCGGCGTCGTC
GCCCTGTTCGGCAACGAGCACTTCCGCACTCTGCCTTACTACCTGTATCAACAGATCGGTGCCTATCGCAGCAATGATGG
CGCGGTCACCGCCCTGCTGTTGCTGCTGTTATGCTTCCTGCTGTTTACCCTGATTGAACGCTTACCGGGCCGCCATGATA
GAGCTTGA

Upstream 100 bases:

>100_bases
CCCAGCTGCCGGCCGGGTTTGAGCAAATGACCGTACCGCAGACCGCGTTGCAATACAGCGCTGAAGAAGTGGCCAAACAA
CGCGGCAACTGGATCCGGGC

Downstream 100 bases:

>100_bases
AAAACTGACCTACCTGTATGAACACCTGCCGATGCGCTTTGACCTGCGCATTGCGCCTGGCGAACGTGTTGCGGTACTTG
GCCCCAGCGGCGCGGGCAAA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MANRRQPLIPRWLWPGLLASGLILTIAVLAMGSLWRHAPESDWQGLWQDSYLWHVVRFTFWQALLSALISVLPAILLARA
LFRRRFPGRQLLLRLCAMTLVLPVLVAVFGLLSVYGRQGWLAAICGWLGINYSFSPYGLQGILLAHMFFNLPLATRLLLQ
ALENIPVEQRQLAAQLGMSGRQQFRFVEWPALRRQILPSGALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
SRAALLALIQLACCLGLVVLSQRLSQALPVGNTHAHIWRNPEDSLWRRVSDFLLIAAALLLLLPPLLAVIADGANQSLVS
VLQQPVLWQALFTSLRIALGAGVLCVLLTMMLLWSSRELKLQQRLRWGQRLELSGMVILAMPGIVLATGFFLLLSDTVGL
PQSPYALVILTNALMAVPYALKVLENPMRDLAERYNPLCLSLDIHGWQRLRLIELKALRRPLAQALAFACVLSIGDFGVV
ALFGNEHFRTLPYYLYQQIGAYRSNDGAVTALLLLLLCFLLFTLIERLPGRHDRA

Sequences:

>Translated_535_residues
MANRRQPLIPRWLWPGLLASGLILTIAVLAMGSLWRHAPESDWQGLWQDSYLWHVVRFTFWQALLSALISVLPAILLARA
LFRRRFPGRQLLLRLCAMTLVLPVLVAVFGLLSVYGRQGWLAAICGWLGINYSFSPYGLQGILLAHMFFNLPLATRLLLQ
ALENIPVEQRQLAAQLGMSGRQQFRFVEWPALRRQILPSGALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
SRAALLALIQLACCLGLVVLSQRLSQALPVGNTHAHIWRNPEDSLWRRVSDFLLIAAALLLLLPPLLAVIADGANQSLVS
VLQQPVLWQALFTSLRIALGAGVLCVLLTMMLLWSSRELKLQQRLRWGQRLELSGMVILAMPGIVLATGFFLLLSDTVGL
PQSPYALVILTNALMAVPYALKVLENPMRDLAERYNPLCLSLDIHGWQRLRLIELKALRRPLAQALAFACVLSIGDFGVV
ALFGNEHFRTLPYYLYQQIGAYRSNDGAVTALLLLLLCFLLFTLIERLPGRHDRA
>Mature_534_residues
ANRRQPLIPRWLWPGLLASGLILTIAVLAMGSLWRHAPESDWQGLWQDSYLWHVVRFTFWQALLSALISVLPAILLARAL
FRRRFPGRQLLLRLCAMTLVLPVLVAVFGLLSVYGRQGWLAAICGWLGINYSFSPYGLQGILLAHMFFNLPLATRLLLQA
LENIPVEQRQLAAQLGMSGRQQFRFVEWPALRRQILPSGALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDLS
RAALLALIQLACCLGLVVLSQRLSQALPVGNTHAHIWRNPEDSLWRRVSDFLLIAAALLLLLPPLLAVIADGANQSLVSV
LQQPVLWQALFTSLRIALGAGVLCVLLTMMLLWSSRELKLQQRLRWGQRLELSGMVILAMPGIVLATGFFLLLSDTVGLP
QSPYALVILTNALMAVPYALKVLENPMRDLAERYNPLCLSLDIHGWQRLRLIELKALRRPLAQALAFACVLSIGDFGVVA
LFGNEHFRTLPYYLYQQIGAYRSNDGAVTALLLLLLCFLLFTLIERLPGRHDRA

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=535, Percent_Identity=74.5794392523364, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI87082099, Length=233, Percent_Identity=27.4678111587983, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59698; Mature: 59567

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRRQPLIPRWLWPGLLASGLILTIAVLAMGSLWRHAPESDWQGLWQDSYLWHVVRFTF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHH
WQALLSALISVLPAILLARALFRRRFPGRQLLLRLCAMTLVLPVLVAVFGLLSVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LAAICGWLGINYSFSPYGLQGILLAHMFFNLPLATRLLLQALENIPVEQRQLAAQLGMSG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
RQQFRFVEWPALRRQILPSGALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
HHHCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCH
SRAALLALIQLACCLGLVVLSQRLSQALPVGNTHAHIWRNPEDSLWRRVSDFLLIAAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
LLLPPLLAVIADGANQSLVSVLQQPVLWQALFTSLRIALGAGVLCVLLTMMLLWSSRELK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQQRLRWGQRLELSGMVILAMPGIVLATGFFLLLSDTVGLPQSPYALVILTNALMAVPYA
HHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LKVLENPMRDLAERYNPLCLSLDIHGWQRLRLIELKALRRPLAQALAFACVLSIGDFGVV
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALFGNEHFRTLPYYLYQQIGAYRSNDGAVTALLLLLLCFLLFTLIERLPGRHDRA
EEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ANRRQPLIPRWLWPGLLASGLILTIAVLAMGSLWRHAPESDWQGLWQDSYLWHVVRFTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHH
WQALLSALISVLPAILLARALFRRRFPGRQLLLRLCAMTLVLPVLVAVFGLLSVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LAAICGWLGINYSFSPYGLQGILLAHMFFNLPLATRLLLQALENIPVEQRQLAAQLGMSG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
RQQFRFVEWPALRRQILPSGALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
HHHCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCH
SRAALLALIQLACCLGLVVLSQRLSQALPVGNTHAHIWRNPEDSLWRRVSDFLLIAAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
LLLPPLLAVIADGANQSLVSVLQQPVLWQALFTSLRIALGAGVLCVLLTMMLLWSSRELK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQQRLRWGQRLELSGMVILAMPGIVLATGFFLLLSDTVGLPQSPYALVILTNALMAVPYA
HHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LKVLENPMRDLAERYNPLCLSLDIHGWQRLRLIELKALRRPLAQALAFACVLSIGDFGVV
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALFGNEHFRTLPYYLYQQIGAYRSNDGAVTALLLLLLCFLLFTLIERLPGRHDRA
EEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]