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The map label for this gene is ilvB [H]

Identifier: 157368979

GI number: 157368979

Start: 815331

End: 817025

Strand: Reverse

Name: ilvB [H]

Synonym: Spro_0734

Alternate gene names: 157368979

Gene position: 817025-815331 (Counterclockwise)

Preceding gene: 157368980

Following gene: 157368978

Centisome position: 14.99

GC content: 61.0

Gene sequence:

>1695_bases
ATGGCAATTTCGGGCACGCCACAGCAAGGCAACAGCTTCACCGGCGCACAGTTGATCGTCCACCTGTTGGAACGTCAGGG
CATCACCACCGTTGCCGGTATTCCCGGCGGCGCTGCACTGCCGCTGTATGACGCACTGAGCCAAAGCACCCGCATTCATC
ACGTACTGGCCCGCCACGAACAGGGCGCGGGCTTTATGGCACAGGGCATGGCACGCGCCAATGGCAAGGCGGCGGTGTGC
ATCGCCTCCAGCGGGCCGGGGGCCACCAACCTGGTCACCGCTATCGCCGACGCCAAGCTCGATTCCATCCCGCTGGTGTG
TATCACCGGCCAGGTGCCGTCTTCGATGATCGGTACCGACGCCTTCCAGGAAGTGGATACCTACGGCATCTCGATTCCTA
TCACCAAGCACAACCATTTGGTTCGCGATATCCGTGAACTGCCGCAGGTGATTTGCGACGCGTTCCGTATTGCCGAATCC
GGCCGCCCTGGCCCGGTGTGGATCGACGTGCCGAAAGACGTGCAAACCGCGGTGATCACTCTGGATGAACTGCCGCCGAT
AGCTATTCCCGACGCGGCACCGGCCTTTGAAGCCTCGGCGGTAGCGCAGGCCGCCGCGATGATCAATCAGGCCAAACGCC
CGGTATTGTATCTGGGTGGCGGCATCATCTGCGCCAATGCTCACCAACAGGCGATTGAGCTGGCGGAACGCGCCAACCTG
CCGACCACCATGACGCTGATGGCGTTGGGTGCCATGCCGGTGCAGCACCCGCTGTCGCTGGGTATGCTGGGGATGCATGC
CGCACGCAGCACCAACTTTATTCTGCAAGAGGCCGATCTGCTGATCGTACTGGGTGCCCGCTTTGACGATCGGGCGATTG
GCAAAACCGAACAGTTCTGCCCGAACGCCGCCATCATTCACGTGGATATCGACCGTGCCGAACTGGGCAAGGTGAAGCAG
GCCAACATTGCCATTCACGCCGACGTTGGCCAGGTATTGCAACAGCTGTTACCACAGATTGAGGCACAGCCGCGCAGCGA
ATGGCTGGACACCGTCAACGGCCTGAAGCGCGAGTTTCCGTTCAGCATGCCGAACGCCGAAGACCCTCTGAGCCACTATG
GGCTGGTGCTGGCTGCTGCGCGCTGCGTGGATGAAAGCGCCATTATCACCACCGACGTCGGCCAGCATCAGATGTGGGTG
GCGCAGGCCTACCCGCTGAGCCGTCCGCGCCAGTGGCTGACCTCCGGCGGATTGGGCACCATGGGCTTCGGCCTGCCTGC
AGCGATAGGTGCAGCCCTGGCCGAACCGCAGCGCAAGGTGCTGTGCTTCTCCGGTGACGGCAGCCTGATGATGAACATTC
AGGAAATGGCCACCGCCGTCGAGCATGATCTGGACGTGAAAATTATCCTGATGAACAACCAGGCGTTGGGGCTGGTGCAT
CAGCAGCAAACGCTGTTCTACCAGCAGCGCATCTTTGCCGCCGCCTATCCCAAGCGTACCGACTTTATCAAGATTGCCGC
CGGTTTCGGGCTGGACACCTGTGATTTGAACGCCGCAGAAGATCCGCAAGCCGCGCTGGCCGAGGCGATCCAACGGCCTG
GCCCTTGTTTGATCCACGCGCTGATTGATATCAATGAAAAAGTGTTCCCGATGGTGCCGCCGGGTGCCGCCAACATCGAC
ATGATTGGAGAATAA

Upstream 100 bases:

>100_bases
ACGCATCGATTCCCAAACCCCGCCGGCGCAAACCGGGCGGGGTTTTTAGTTTCAGTCCCCTGCCCAGAAAAGCGAACCGG
CCTTGAGAAAGGATAGGAAC

Downstream 100 bases:

>100_bases
GCCATGTCAGAACAATTGAATAATCCACAGGTGATCCTGGAACTGACGGTACGTAACCATCCCGGCGTGATGTCGCACGT
GTGCGGCCTGTTTGCCCGCC

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MAISGTPQQGNSFTGAQLIVHLLERQGITTVAGIPGGAALPLYDALSQSTRIHHVLARHEQGAGFMAQGMARANGKAAVC
IASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNHLVRDIRELPQVICDAFRIAES
GRPGPVWIDVPKDVQTAVITLDELPPIAIPDAAPAFEASAVAQAAAMINQAKRPVLYLGGGIICANAHQQAIELAERANL
PTTMTLMALGAMPVQHPLSLGMLGMHAARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAAIIHVDIDRAELGKVKQ
ANIAIHADVGQVLQQLLPQIEAQPRSEWLDTVNGLKREFPFSMPNAEDPLSHYGLVLAAARCVDESAIITTDVGQHQMWV
AQAYPLSRPRQWLTSGGLGTMGFGLPAAIGAALAEPQRKVLCFSGDGSLMMNIQEMATAVEHDLDVKIILMNNQALGLVH
QQQTLFYQQRIFAAAYPKRTDFIKIAAGFGLDTCDLNAAEDPQAALAEAIQRPGPCLIHALIDINEKVFPMVPPGAANID
MIGE

Sequences:

>Translated_564_residues
MAISGTPQQGNSFTGAQLIVHLLERQGITTVAGIPGGAALPLYDALSQSTRIHHVLARHEQGAGFMAQGMARANGKAAVC
IASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNHLVRDIRELPQVICDAFRIAES
GRPGPVWIDVPKDVQTAVITLDELPPIAIPDAAPAFEASAVAQAAAMINQAKRPVLYLGGGIICANAHQQAIELAERANL
PTTMTLMALGAMPVQHPLSLGMLGMHAARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAAIIHVDIDRAELGKVKQ
ANIAIHADVGQVLQQLLPQIEAQPRSEWLDTVNGLKREFPFSMPNAEDPLSHYGLVLAAARCVDESAIITTDVGQHQMWV
AQAYPLSRPRQWLTSGGLGTMGFGLPAAIGAALAEPQRKVLCFSGDGSLMMNIQEMATAVEHDLDVKIILMNNQALGLVH
QQQTLFYQQRIFAAAYPKRTDFIKIAAGFGLDTCDLNAAEDPQAALAEAIQRPGPCLIHALIDINEKVFPMVPPGAANID
MIGE
>Mature_563_residues
AISGTPQQGNSFTGAQLIVHLLERQGITTVAGIPGGAALPLYDALSQSTRIHHVLARHEQGAGFMAQGMARANGKAAVCI
ASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNHLVRDIRELPQVICDAFRIAESG
RPGPVWIDVPKDVQTAVITLDELPPIAIPDAAPAFEASAVAQAAAMINQAKRPVLYLGGGIICANAHQQAIELAERANLP
TTMTLMALGAMPVQHPLSLGMLGMHAARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAAIIHVDIDRAELGKVKQA
NIAIHADVGQVLQQLLPQIEAQPRSEWLDTVNGLKREFPFSMPNAEDPLSHYGLVLAAARCVDESAIITTDVGQHQMWVA
QAYPLSRPRQWLTSGGLGTMGFGLPAAIGAALAEPQRKVLCFSGDGSLMMNIQEMATAVEHDLDVKIILMNNQALGLVHQ
QQTLFYQQRIFAAAYPKRTDFIKIAAGFGLDTCDLNAAEDPQAALAEAIQRPGPCLIHALIDINEKVFPMVPPGAANIDM
IGE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=493, Percent_Identity=27.9918864097363, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI21361361, Length=554, Percent_Identity=25.812274368231, Blast_Score=143, Evalue=5e-34,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=75.531914893617, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=39.9644760213144, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=528, Percent_Identity=32.7651515151515, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=27.5547445255474, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=25.6781193490054, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=568, Percent_Identity=25.3521126760563, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=25.3097345132743, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=25.3097345132743, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=42.4398625429553, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=543, Percent_Identity=25.9668508287293, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321524, Length=490, Percent_Identity=22.2448979591837, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=23.265306122449, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=29.8136645962733, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60051; Mature: 59920

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISGTPQQGNSFTGAQLIVHLLERQGITTVAGIPGGAALPLYDALSQSTRIHHVLARHE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
QGAGFMAQGMARANGKAAVCIASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTD
CCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCH
AFQEVDTYGISIPITKHNHLVRDIRELPQVICDAFRIAESGRPGPVWIDVPKDVQTAVIT
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEE
LDELPPIAIPDAAPAFEASAVAQAAAMINQAKRPVLYLGGGIICANAHQQAIELAERANL
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHCCC
PTTMTLMALGAMPVQHPLSLGMLGMHAARSTNFILQEADLLIVLGARFDDRAIGKTEQFC
CHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCHHHHC
PNAAIIHVDIDRAELGKVKQANIAIHADVGQVLQQLLPQIEAQPRSEWLDTVNGLKREFP
CCCEEEEEECCHHHHCCEEECCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
FSMPNAEDPLSHYGLVLAAARCVDESAIITTDVGQHQMWVAQAYPLSRPRQWLTSGGLGT
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHCCCCCC
MGFGLPAAIGAALAEPQRKVLCFSGDGSLMMNIQEMATAVEHDLDVKIILMNNQALGLVH
CCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEECCCEEEEEH
QQQTLFYQQRIFAAAYPKRTDFIKIAAGFGLDTCDLNAAEDPQAALAEAIQRPGPCLIHA
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
LIDINEKVFPMVPPGAANIDMIGE
EECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AISGTPQQGNSFTGAQLIVHLLERQGITTVAGIPGGAALPLYDALSQSTRIHHVLARHE
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
QGAGFMAQGMARANGKAAVCIASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTD
CCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCH
AFQEVDTYGISIPITKHNHLVRDIRELPQVICDAFRIAESGRPGPVWIDVPKDVQTAVIT
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEE
LDELPPIAIPDAAPAFEASAVAQAAAMINQAKRPVLYLGGGIICANAHQQAIELAERANL
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHCCC
PTTMTLMALGAMPVQHPLSLGMLGMHAARSTNFILQEADLLIVLGARFDDRAIGKTEQFC
CHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCHHHHC
PNAAIIHVDIDRAELGKVKQANIAIHADVGQVLQQLLPQIEAQPRSEWLDTVNGLKREFP
CCCEEEEEECCHHHHCCEEECCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
FSMPNAEDPLSHYGLVLAAARCVDESAIITTDVGQHQMWVAQAYPLSRPRQWLTSGGLGT
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHCCCCCC
MGFGLPAAIGAALAEPQRKVLCFSGDGSLMMNIQEMATAVEHDLDVKIILMNNQALGLVH
CCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEECCCEEEEEH
QQQTLFYQQRIFAAAYPKRTDFIKIAAGFGLDTCDLNAAEDPQAALAEAIQRPGPCLIHA
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
LIDINEKVFPMVPPGAANIDMIGE
EECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]