Click here to switch to the map view.

The map label for this gene is est [H]

Identifier: 157368631

GI number: 157368631

Start: 431324

End: 432442

Strand: Direct

Name: est [H]

Synonym: Spro_0384

Alternate gene names: 157368631

Gene position: 431324-432442 (Clockwise)

Preceding gene: 157368630

Following gene: 157368633

Centisome position: 7.92

GC content: 57.55

Gene sequence:

>1119_bases
ATGAAATACCTGTCTGTAAGGACGCCCCTGCTGCGGGCTGCTGCTGTGTCCTGTCTGTTTTTGATGCCGCTGTCGGGTCA
GGCCGCAGAGGACAATGCTGCCTGGCTGAAGCTGGAAGCGGAGCAGATCCAGAAAAATTCCATACCCGGACCCCGCCATG
TCCCGGCAAAAATTCTGCCGGCTCCGGCCGGCATCAGTCCGGAGCTGAAAAAAGATATCGAAGCACCCTATAATTCCCCG
CGGTGGTATGGCGATCATGCCAGCACCGTGGCGGAATGGCAGTCAATGATCGACCGTTCCACAGCGGCCACGCTGAAGGT
CGTTCCGGTGTTACGCAAGGCGCTGGACGTGTCAGTCAGGGAAGAAAGTATGGGGGGGGTGCCGGTATACGAAATCAGGC
CTGCCGGTTACCGGGATAACGGAAAGGTGGTACTGTATATCCACGGCGGCGGATATGTTTATAACCCGGGGATAACAGGA
ACACCTGAAGCCATGCTGATGGCCGCCTTTACCGGTTACCGGGTCATTTCTGTAGACTATCGCATGCCGCCGTCACACCC
CTATCCGGCCGCACTGGATGATGTTTTCAGCGTTTACAAAGCGCTTATCAGCCAGCAGGATCCCGGCTCCGTAGCCGTTT
TCGGCACCTCGGCAGGAGGCGGCCTTGCCATGTCCCTGCTTCTCCGGGCCAGAAACGAAAAAGTGGCGCTGCCGGCAGCG
GCCGGACTGGGCACTCCCTGGATGGATCTGACGCCGGGCGGCGGAGGTGACACCATGAACACGCTGGAGTGGGTGGATAA
CACGCTTATCAGCGGGCGGGGTTACATTGCCCGATCTGCCCTGCTGTATGCAGACGGACATTCCCTGAAGGATCCGTATA
TTTCTCCTCTGTTTGGAGACTTTACCGGCCTTCCGCCGGTCATGATTATCAGCGGAACCCGCGATCTTTTCCTGAGCCAG
GGCGTTCTTACCCAGCGTAAGCTGCGTGAAGCCGGTAATGTCGCCAGCCTGCAACTGTGGGACGGCATGTCGCATGCGCA
GTACAATAACTACCAGGCGCCTGAATCACGACAGGTTTTCCAGGAAGTCGGGAAGTTTTTTACCGAATACTTACGCTGA

Upstream 100 bases:

>100_bases
CCCATGGAGTATTAAACAATTTGTTGTACTTTGTCATATGACAATATATCTTCTGCCTTAAACTTAACATTCATGTAACT
TTTTATCAGGGAAATTAACA

Downstream 100 bases:

>100_bases
CTGATAAAGGGAGCAACTGTGCTCCCTTACTTCTCGCTTAATGAATGCCATGTATTTTCTGTGAATGCTACCGCCAGCGA
AAGCGATATCACAATCAGGC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKYLSVRTPLLRAAAVSCLFLMPLSGQAAEDNAAWLKLEAEQIQKNSIPGPRHVPAKILPAPAGISPELKKDIEAPYNSP
RWYGDHASTVAEWQSMIDRSTAATLKVVPVLRKALDVSVREESMGGVPVYEIRPAGYRDNGKVVLYIHGGGYVYNPGITG
TPEAMLMAAFTGYRVISVDYRMPPSHPYPAALDDVFSVYKALISQQDPGSVAVFGTSAGGGLAMSLLLRARNEKVALPAA
AGLGTPWMDLTPGGGGDTMNTLEWVDNTLISGRGYIARSALLYADGHSLKDPYISPLFGDFTGLPPVMIISGTRDLFLSQ
GVLTQRKLREAGNVASLQLWDGMSHAQYNNYQAPESRQVFQEVGKFFTEYLR

Sequences:

>Translated_372_residues
MKYLSVRTPLLRAAAVSCLFLMPLSGQAAEDNAAWLKLEAEQIQKNSIPGPRHVPAKILPAPAGISPELKKDIEAPYNSP
RWYGDHASTVAEWQSMIDRSTAATLKVVPVLRKALDVSVREESMGGVPVYEIRPAGYRDNGKVVLYIHGGGYVYNPGITG
TPEAMLMAAFTGYRVISVDYRMPPSHPYPAALDDVFSVYKALISQQDPGSVAVFGTSAGGGLAMSLLLRARNEKVALPAA
AGLGTPWMDLTPGGGGDTMNTLEWVDNTLISGRGYIARSALLYADGHSLKDPYISPLFGDFTGLPPVMIISGTRDLFLSQ
GVLTQRKLREAGNVASLQLWDGMSHAQYNNYQAPESRQVFQEVGKFFTEYLR
>Mature_372_residues
MKYLSVRTPLLRAAAVSCLFLMPLSGQAAEDNAAWLKLEAEQIQKNSIPGPRHVPAKILPAPAGISPELKKDIEAPYNSP
RWYGDHASTVAEWQSMIDRSTAATLKVVPVLRKALDVSVREESMGGVPVYEIRPAGYRDNGKVVLYIHGGGYVYNPGITG
TPEAMLMAAFTGYRVISVDYRMPPSHPYPAALDDVFSVYKALISQQDPGSVAVFGTSAGGGLAMSLLLRARNEKVALPAA
AGLGTPWMDLTPGGGGDTMNTLEWVDNTLISGRGYIARSALLYADGHSLKDPYISPLFGDFTGLPPVMIISGTRDLFLSQ
GVLTQRKLREAGNVASLQLWDGMSHAQYNNYQAPESRQVFQEVGKFFTEYLR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 40367; Mature: 40367

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLSVRTPLLRAAAVSCLFLMPLSGQAAEDNAAWLKLEAEQIQKNSIPGPRHVPAKILP
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEC
APAGISPELKKDIEAPYNSPRWYGDHASTVAEWQSMIDRSTAATLKVVPVLRKALDVSVR
CCCCCCHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EESMGGVPVYEIRPAGYRDNGKVVLYIHGGGYVYNPGITGTPEAMLMAAFTGYRVISVDY
HHCCCCCCEEEECCCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCEEEEEEE
RMPPSHPYPAALDDVFSVYKALISQQDPGSVAVFGTSAGGGLAMSLLLRARNEKVALPAA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEECEEC
AGLGTPWMDLTPGGGGDTMNTLEWVDNTLISGRGYIARSALLYADGHSLKDPYISPLFGD
CCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCCCHHHC
FTGLPPVMIISGTRDLFLSQGVLTQRKLREAGNVASLQLWDGMSHAQYNNYQAPESRQVF
CCCCCCEEEEECCHHHHHHCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHH
QEVGKFFTEYLR
HHHHHHHHHHHC
>Mature Secondary Structure
MKYLSVRTPLLRAAAVSCLFLMPLSGQAAEDNAAWLKLEAEQIQKNSIPGPRHVPAKILP
CCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEC
APAGISPELKKDIEAPYNSPRWYGDHASTVAEWQSMIDRSTAATLKVVPVLRKALDVSVR
CCCCCCHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EESMGGVPVYEIRPAGYRDNGKVVLYIHGGGYVYNPGITGTPEAMLMAAFTGYRVISVDY
HHCCCCCCEEEECCCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHCCCEEEEEEE
RMPPSHPYPAALDDVFSVYKALISQQDPGSVAVFGTSAGGGLAMSLLLRARNEKVALPAA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEECEEC
AGLGTPWMDLTPGGGGDTMNTLEWVDNTLISGRGYIARSALLYADGHSLKDPYISPLFGD
CCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCCCHHHC
FTGLPPVMIISGTRDLFLSQGVLTQRKLREAGNVASLQLWDGMSHAQYNNYQAPESRQVF
CCCCCCEEEEECCHHHHHHCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHH
QEVGKFFTEYLR
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]